Variant-specific SARS-CoV-2 shedding rates in wastewater

The Science of The Total Environment, Год журнала: 2022, Номер 857, С. 159165 - 159165

Опубликована: Окт. 3, 2022

Язык: Английский

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Use of wastewater surveillance for early detection of Alpha and Epsilon SARS-CoV-2 variants of concern and estimation of overall COVID-19 infection burden

The Science of The Total Environment, Год журнала: 2022, Номер 835, С. 155410 - 155410

Опубликована: Апрель 22, 2022

A decline in diagnostic testing for SARS-CoV-2 is expected to delay the tracking of COVID-19 variants concern and interest United States. We hypothesize that wastewater surveillance programs provide an effective alternative detecting emerging assessing incidence, particularly when clinical limited. Here, we analyzed RNA from eight locations across Southern Nevada between March 2020 April 2021. Trends concentrations (ranging 4.3 log

Язык: Английский

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Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach

PLoS ONE, Год журнала: 2022, Номер 17(6), С. e0270168 - e0270168

Опубликована: Июнь 17, 2022

Clinical testing of children in schools is challenging, with economic implications limiting its frequent use as a monitoring tool the risks assumed by and staff during COVID-19 pandemic. Here, wastewater-based epidemiology approach has been used to monitor 16 (10 primary, 5 secondary 1 post-16 further education) England. A total 296 samples over 9 weeks have analysed for N1 E genes using qPCR methods. Of returned, 47.3% were positive one or both detection frequency line respective local community. WBE offers low cost, non-invasive supplementing clinical can provide longitudinal insights that are impractical traditional testing.

Язык: Английский

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Citywide wastewater SARS-CoV-2 levels strongly correlated with multiple disease surveillance indicators and outcomes over three COVID-19 waves

The Science of The Total Environment, Год журнала: 2022, Номер 855, С. 158967 - 158967

Опубликована: Сен. 24, 2022

Язык: Английский

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Identification and genome sequencing of an influenza H3N2 variant in wastewater from elementary schools during a surge of influenza A cases in Las Vegas, Nevada

The Science of The Total Environment, Год журнала: 2023, Номер 872, С. 162058 - 162058

Опубликована: Фев. 8, 2023

Real-time surveillance of infectious diseases at schools or in communities is often hampered by delays reporting due to resource limitations and infrastructure issues. By incorporating quantitative PCR genome sequencing, wastewater has been an effective complement public health the community building-scale for pathogens such as poliovirus, SARS-CoV-2, even monkeypox virus. In this study, we asked whether programs elementary could be leveraged detect RNA from influenza viruses shed wastewater. We monitored A B viral six January May 2022. Quantitative led identification three schools, which coincided with lifting COVID-19 restrictions a surge infections Las Vegas, Nevada, USA. performed sequencing RNA, leading 2021–2022 vaccine-resistant (H3N2) 3C.2a1b.2a.2 subclade. next tested samples treatment plant that serviced but were unable presence A/B RNA. Together, our results demonstrate utility near-source detection local transmission potential investigated further paired school-level incidence data.

Язык: Английский

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COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities

iScience, Год журнала: 2023, Номер 26(7), С. 107019 - 107019

Опубликована: Июнь 9, 2023

Язык: Английский

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Degradation rates influence the ability of composite samples to represent 24-hourly means of SARS-CoV-2 and other microbiological target measures in wastewater

The Science of The Total Environment, Год журнала: 2023, Номер 867, С. 161423 - 161423

Опубликована: Янв. 6, 2023

Язык: Английский

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Structured Ethical Review for Wastewater-Based Testing in Support of Public Health

Environmental Science & Technology, Год журнала: 2023, Номер 57(35), С. 12969 - 12980

Опубликована: Авг. 23, 2023

Wastewater-based testing (WBT) for SARS-CoV-2 has rapidly expanded over the past three years due to its ability provide a comprehensive measurement of disease prevalence independent clinical testing. The development and simultaneous application WBT measured biomarkers research activities pursuit public health goals, both areas with well-established ethical frameworks. Currently, practitioners do not employ standardized review process, introducing potential adverse outcomes professionals community members. To address this deficiency, an interdisciplinary workshop developed framework structured WBT. employed consensus approach create as set 11 questions derived from primarily guidance. This study retrospectively applied these monitoring programs covering emergent phase pandemic (3/2020-2/2022 (

Язык: Английский

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Simultaneous detection and quantification of multiple pathogen targets in wastewater

PLOS Water, Год журнала: 2024, Номер 3(2), С. e0000224 - e0000224

Опубликована: Фев. 1, 2024

Wastewater-based epidemiology has emerged as a critical tool for public health surveillance, building on decades of environmental surveillance work pathogens such poliovirus. Work to date been limited monitoring single pathogen or small numbers in targeted studies; however, few studies consider simultaneous quantitative analysis wide variety pathogens, which could greatly increase the utility wastewater surveillance. We developed novel multi-pathogen approach (35 targets including bacteria, viruses, protozoa, and helminths) using TaqMan Array Cards (TAC) applied method concentrated samples collected at four treatment plants Atlanta, GA from February October 2020. From sewersheds serving approximately 2 million people, we detected range many expected find (e.g., enterotoxigenic E . coli Giardia 97% 29 stable concentrations) well unexpected Strongyloides stercoralis (a human threadworm rarely observed USA). Other notable detections included SARS-CoV-2, but also several that are not commonly like Acanthamoeba spp., Balantidium , Entamoeba histolytica astrovirus, norovirus, sapovirus. Our data suggest broad expanding scope enteric wastewaters, with potential application settings where quantification fecal waste streams can inform selection control measures limit infections.

Язык: Английский

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Wastewater surveillance for viral pathogens: A tool for public health

Heliyon, Год журнала: 2024, Номер 10(13), С. e33873 - e33873

Опубликована: Июнь 29, 2024

A focus on water quality has intensified globally, considering its critical role in sustaining life and ecosystems. Wastewater, reflecting societal development, profoundly impacts public health. Wastewater-based epidemiology (WBE) emerged as a surveillance tool for detecting outbreaks early, monitoring infectious disease trends, providing real-time insights, particularly vulnerable communities. WBE aids tracking pathogens, including viruses, sewage, offering comprehensive understanding of community health lifestyle habits. With the rise global COVID-19 cases, gained prominence, aiding SARS-CoV-2 levels worldwide. Despite advancements treatment, poorly treated wastewater discharge remains threat, amplifying spread water-, sanitation-, hygiene (WaSH)-related diseases. WBE, serving complementary surveillance, is pivotal community-level viral infections. However, there untapped potential to expand surveillance. This review emphasizes importance link between health, highlighting need further integration into management.

Язык: Английский

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