Insects,
Год журнала:
2022,
Номер
13(12), С. 1091 - 1091
Опубликована: Ноя. 27, 2022
Mitochondria
are
the
leading
organelle
for
energy
metabolism.
The
toxic
effects
of
environmental
toxicants
on
mitochondrial
morphology,
metabolism,
and
their
determination
cell
fate
have
already
been
broadly
studied.
However,
minimal
research
exists
such
as
pesticides
metabolism
at
in
vitro
subcellular
level,
particularly
from
an
omics
perspectives
(e.g.,
metabolomics).
Here,
German
cockroach
(Blattella
germanica)
was
fed
diets
with
(0.01
0.001
mg/mL)
without
abamectin,
highly
purified
fat
body
mitochondria
were
isolated.
Swelling
measurement
confirmed
abnormal
swelling
caused
by
abamectin
stress.
activity
two
key
metabolism-related
enzymes,
namely
succinic
dehydrogenase
isocitrate
dehydrogenase,
significantly
affected.
metabolomic
responses
isolated
to
analyzed
via
untargeted
liquid
chromatography/mass
spectrometry
metabolomics
technology.
Fifty-two
differential
metabolites
(DMs)
identified
between
mg/mL
abamectin-fed
control
groups.
Many
these
DMs
enriched
pathways
involved
ATP
production
consumption
oxidative
phosphorylation,
TCA
cycle,
pentose
phosphate
pathway).
Nineteen
typically
related
This
study
is
valuable
further
understanding
toxicology
under
toxicants,
its
level.
Arabian Journal of Chemistry,
Год журнала:
2022,
Номер
15(10), С. 104138 - 104138
Опубликована: Июль 19, 2022
Allium
macrostemon
Bge.
(AMB)
and
chinense
G.
Don
(ACGD),
both
named
Allii
Macrostemonis
Bulbus
or
Xiebai
in
Chinese,
belong
to
the
same
genus.
They
are
used
as
edible
vegetables
medicinal
herbs
for
treatment
of
atherosclerosis.
So
far,
comparative
analysis
spatial
metabolomes
platelet
aggregation
function
steroidal
saponins
has
rarely
been
performed.
In
this
study,
high
mass
resolution
matrix-assisted
laser
desorption/ionization
time-of-flight
imaging
spectrometry
(MALDI-TOF
MSI)
were
performed
on
fresh
bulbs
these
two
distribution
saponins,
ultra-high-performance
liquid
chromatography
tandem
triple
quadrupole
(UHPLC/TQD-MS)
was
quantify
their
content
levels.
Platelet
inhibitory
activity
induced
by
arachidonic
acid,
adenosine
diphosphate
collagen
fully
investigated.
A
total
53
differential
variables
with
compounds
identified
tentatively
characterized
between
AMB
ACGD
samples
UHPLC/TQD-MS.
Furthermore,
almost
distributed
tunic
outer
scale
regions
AMB,
while
they
rich
whole
leaf
scale,
developing
flower
bud
bulbs.
Quantitative
results
demonstrated
several
unique
components
(macrostemonoside
I,
timosaponin
B-II,
macrostemonoside
F,
etc.)
(chinenoside
chinenoside
II,
IV,
etc.).
Moreover,
spirostanol
exhibited
stronger
than
furostanol
structure
relationship
further
summarized.
conclusion,
high-mass
MALDI
full-scan
MSI
provides
abundant
information
ACGD.
And
we
finished
discussion
characteristics.
These
findings
would
benefit
understanding
safety
effectiveness
use.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(20), С. 7863 - 7871
Опубликована: Май 9, 2023
Understanding
the
metabolic
disorders
induced
by
nano-
and
microplastics
in
aquatic
organisms
at
molecular
level
could
help
us
understand
potential
toxicity
of
more
thoroughly
provide
a
fundamental
scientific
basis
for
regulating
usage
management
plastic
products.
In
this
research,
effect
polypropylene
nanoplastics
(PP-NPs)
(PP-MPs)
on
metabolites
tilapia
liver
was
comprehensively
investigated
internal
extractive
electrospray
ionization
mass
spectrometry
(iEESI-MS).
A
partial
least-squares
discriminant
analysis
(PLS-DA)
one-component
variance
(ANOVA)
were
used
selecting
46
differential
metabolites,
including
phospholipids,
amino
acids,
peptides,
carbohydrates,
alkaloids,
purines,
pyrimidines,
nucleosides.
Pathway
enrichment
showed
significant
effects
glycerophospholipid
metabolism,
arginine
proline
aminoacyl-tRNA
biosynthesis
after
exposed
to
PP-N/MPs.
Dysregulation
these
is
mainly
reflected
possible
induction
hepatitis,
oxidative
stress,
other
symptoms.
The
application
iEESI-MS
technology
without
sample
pretreatment
study
under
interference
provides
promising
analytical
method
environmental
toxicology
research.
