Viruses, Год журнала: 2024, Номер 16(11), С. 1657 - 1657
Опубликована: Окт. 23, 2024
This study reports the use of Nanotrap
Язык: Английский
The Science of The Total Environment, Год журнала: 2024, Номер 945, С. 173862 - 173862
Опубликована: Июнь 12, 2024
Wastewater surveillance (WWS) has received significant attention as a rapid, sensitive, and cost-effective tool for monitoring various pathogens in community. WWS is employed to assess the spatial temporal trends of diseases identify their early appearances reappearances, well detect novel mutated variants. However, shedding rates vary significantly depending on factors such disease severity, physiology affected individuals, characteristics pathogen. Furthermore, may exhibit differential fate decay kinetics sewerage system. Variable affect detection wastewater. This influence interpretation results conclusions studies. When selecting pathogen WWS, it essential consider it's specific characteristics. If data are not readily available, fate, decay, should be assessed before conducting surveillance. Alternatively, these can compared those similar which available.
Язык: Английский
Процитировано
19
Current Opinion in Environmental Science & Health, Год журнала: 2023, Номер 33, С. 100458 - 100458
Опубликована: Март 7, 2023
Язык: Английский
Процитировано
30
The Science of The Total Environment, Год журнала: 2024, Номер 926, С. 171401 - 171401
Опубликована: Март 11, 2024
Wastewater comprises multiple pathogens and offers a potential for wastewater-based surveillance (WBS) to track the prevalence of communicable diseases. The Finnish WastPan project aimed establish pandemic preparedness (viruses, bacteria, parasites, fungi), including antimicrobial resistance (AMR). This article outlines WastPan's experiences in this project, criteria target selection, sampling locations, frequency, analysis methods results communication. Target selection relied on epidemiological microbiological evidence practical feasibility. Within framework, wastewater samples were collected between 2021 2023 from 10 treatment plants (WWTPs) covering 40 % Finland's population. WWTP was validated reported cases Extended Spectrum Beta-lactamase-producing bacterial (Escherichia coli Klebsiella pneumoniae) National Infectious Disease Register. workflow included 24-h composite influent samples, with one fraction culture-based (bacteria fungi) rest sample reserved molecular antibiotic genes, parasites). reproducibility monitoring assessed SARS-CoV-2 through inter-laboratory comparisons using N2 N1 assays. Identical protocols applied same-day yielding similar positivity trends two laboratories, but assay achieved significantly higher detection rate (Laboratory 1: 91.5 %; Laboratory 2: 87.4 %) than (76.6 monitored only 2 (McNemar, p < 0.001 Lab 1, = 0.006 2). result indicates that primers assays may impact sensitivity WBS. Overall, current study recommends frequencies population coverage should be based pathogen-specific characteristics. For example, are stable over time need less frequent annual sampling, while those occurring across regions require reduced coverage. Here, successfully piloted WBS pathogens, highlighting significance one-litre community assessing health. infrastructure established COVID-19 is valuable various pathogens. Prioritizing targets optimizes resource utilization, legislative support determination sustained funding advisable future.
Язык: Английский
Процитировано
13
Water Research, Год журнала: 2022, Номер 230, С. 119560 - 119560
Опубликована: Дек. 31, 2022
Язык: Английский
Процитировано
33
Viruses, Год журнала: 2024, Номер 16(3), С. 460 - 460
Опубликована: Март 17, 2024
Tomato Brown Rugose Fruit Virus (ToBRFV) is a plant pathogen that infects important Solanaceae crop species and can dramatically reduce tomato yields. The ToBRFV has rapidly spread around the globe due to its ability escape detection by antiviral host genes which confer resistance other tobamoviruses in plants. development of robust reproducible methods for detecting viruses environment aids tracking reduction transmission. We detected municipal wastewater influent (WWI) samples, likely presence human waste, demonstrating widespread distribution WWI throughout Ontario, Canada. To aid global surveillance efforts, we developed tiled amplicon approach sequence track evolution genomes WWI. Our assay recovers 95.7% 6393 bp RefSeq genome, omitting terminal 5′ 3′ ends. demonstrate our sequencing robust, sensitive, highly specific method recovering genomes. was using existing ARTIC Network resources, including primer design, library prep, read analysis. Additionally, adapted lineage abundance estimation tool, Alcov, estimate clades samples.
Язык: Английский
Процитировано
9
FEMS Microbes, Год журнала: 2024, Номер 5
Опубликована: Янв. 1, 2024
Abstract Wastewater-based epidemiology is now widely used in many countries for the routine monitoring of SARS-CoV-2 and other viruses at a community level. However, efficient sample processing technologies are still under investigation. In this study, we compared performance novel Nanotrap® Microbiome Particles (NMP) concentration method to commonly polyethylene glycol (PEG) precipitation concentrating from wastewater their subsequent quantification sequencing. For this, first spiked with SARS-CoV-2, influenza measles norovirus found that NMP recovered 0.4%–21% them depending on virus type, providing consistent reproducible results. Using PEG methods, monitored A B viruses, RSV, enteroviruses GI GII crAssphage using quantitative PCR (qPCR)-based methods next-generation Good viral recoveries were observed highly abundant both methods; however, was more successful recovery low-abundance present wastewater. Furthermore, samples processed successfully sequenced than those method. Virus enhanced by high volumes when applied. Overall, our results suggest rapid easy targets wastewater, whereas may be suited analysis next generation
Язык: Английский
Процитировано
7
The Science of The Total Environment, Год журнала: 2023, Номер 896, С. 165007 - 165007
Опубликована: Июнь 21, 2023
The effective detection of viruses in aircraft wastewater is crucial to establish surveillance programs for monitoring virus spread via passengers. This study aimed compare the performance two concentration workflows, adsorption-extraction (AE) and Nanotrap® Microbiome A Particles (NMAP), detecting prevalence concentrations 15 endogenous including ssDNA, dsDNA, ssRNA 24 lavatory samples. tested included indicator viruses, four enteric nine respiratory viruses. results showed that cross-assembly phage (crAssphage), human polyomavirus (HPyV), rhinovirus (RhV A), B B) were detected all samples using both workflows. However, enterovirus (EV), norovirus GII (HNoV GII), adenovirus (HAdV), bocavirus (BoV), parechovirus (PeV), epstein-barr (EBV). Influenza (IAV), syncytial (RsV infrequently by hepatitis (HAV), influenza (IBV), A) not any NMAP workflow had greater rates RNA (EV, PeV, RsV than AE workflow, while DNA (HAdV, BoV, EBV) workflow. each was also analyzed, crAssphage highest mean (6.76 log10 GC/12.5 mL) followed HPyV (5.46 mL ranged from 2.48 3.63 mL. Using 5.18 4.20 mL, 2.55 3.74 Significantly higher (p < 0.05) observed when employing comparison Conversely, yielded significantly RhV A, compared findings this can aid selection an appropriate studies contribute development efficient methods.
Язык: Английский
Процитировано
15
The Science of The Total Environment, Год журнала: 2023, Номер 876, С. 162572 - 162572
Опубликована: Март 3, 2023
Язык: Английский
Процитировано
11
The Science of The Total Environment, Год журнала: 2023, Номер 912, С. 168782 - 168782
Опубликована: Ноя. 23, 2023
Язык: Английский
Процитировано
11
Journal of Virological Methods, Год журнала: 2023, Номер 317, С. 114732 - 114732
Опубликована: Апрель 18, 2023
The ongoing COVID-19 pandemic has emphasized the significance of wastewater surveillance in monitoring and tracking spread infectious diseases, including SARS-CoV-2. approach detects genetic fragments from viruses wastewater, which could provide an early warning outbreaks communities. In this study, we determined concentrations four types endogenous viruses, non-enveloped DNA (crAssphage human adenovirus 40/41), RNA (enterovirus), enveloped (SARS-CoV-2) samples using adsorption-extraction (AE) method with electronegative HA membranes different pore sizes (0.22, 0.45, 0.80 µm). Our findings showed that membrane a size µm performed comparably to 0.45 for virus detection/quantitation (repeated measurement one-way ANOVA; p > 0.05). We also recovery efficiencies indigenous crAssphage pepper mild mottle virus, ranging 50% 94% 20% 62%, respectively. results suggest use larger may be beneficial processing sample volumes, particularly environmental waters containing low viruses. This study offers valuable insights into application AE is essential diseases
Язык: Английский
Процитировано
10