Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 19, 2024
Abstract
Background
Multiple
epigenetic
regulatory
mechanisms
exert
critical
roles
in
tumour
development,
understanding
the
interactions
and
impact
of
diverse
modifications
on
gene
expression
cancer
is
crucial
for
development
precision
medicine.
The
main
objective
this
study
was
to
elucidate
intricate
crosstalk
between
N6-methyladenosine
(m6A)
modification
histone
context
non-small
cell
lung
(NSCLC).
Results
Bioinformatics
analyses
experiments
confirmed
that
m6A
methyltransferase
METTL14
significantly
downregulated
NSCLC
tissues,
with
lower
levels
correlating
poorer
overall
survival.
Functional
demonstrated
overexpression
inhibited
proliferation
migration
cells
both
vivo
vitro,
colorimetric
quantification
assay
also
showed
knockdown
notably
reduced
global
cells.
We
using
MeRIP-qPCR
dual-luciferase
reporter
assays
long
noncoding
RNA
LINC02747
targeted
regulated
by
via
modification,
inhibiting
observed
hinder
malignant
progression
modulating
PI3K/Akt
signaling
pathway.
Knockdown
decreased
upregulated
its
expression.
Further
studies
revealed
promoted
methylation
accelerated
decay
mRNA
increased
recognition
"GAACU"
binding
site
YTHDC2.
Additionally,
demethylase
lysine-specific
5B
(KDM5B)
mediated
demethylation
H3
lysine
4
tri-methylation
(H3K4me3)
promoter
region
repressed
transcription,
KDM5B
suppressing
METTL14.
Conclusions
In
summary,
at
transcriptional
level
a
H3K4me3-dependent
manner,
while
modulated
modification.
Our
results
demonstrate
series
regulate
phenotype
cells,
revealing
complex
regulation
involved
occurrence
cancer.
Antioxidants,
Год журнала:
2021,
Номер
11(1), С. 20 - 20
Опубликована: Дек. 22, 2021
The
prominent
cultivation
of
lemongrass
(Cymbopogon
spp.)
relies
on
the
pharmacological
incentives
its
essential
oil.
Lemongrass
oil
(LEO)
carries
a
significant
amount
numerous
bioactive
compounds,
such
as
citral
(mixture
geranial
and
neral),
isoneral,
isogeranial,
geraniol,
geranyl
acetate,
citronellal,
citronellol,
germacrene-D,
elemol,
in
addition
to
other
compounds.
These
components
confer
various
actions
LEO,
including
antifungal,
antibacterial,
antiviral,
anticancer,
antioxidant
properties.
LEO
attributes
are
commercially
exploited
pharmaceutical,
cosmetics,
food
preservations
industries.
Furthermore,
application
treatment
cancer
opens
new
vista
field
therapeutics.
Although
different
have
shown
promising
anticancer
activities
vitro,
their
effects
not
yet
been
assessed
human
system.
Hence,
further
studies
mechanisms
conferred
by
required.
present
review
intends
provide
timely
discussion
relevance
combating
sustaining
healthcare,
well
industry
applications.
Cancers,
Год журнала:
2025,
Номер
17(1), С. 154 - 154
Опубликована: Янв. 6, 2025
Inflammation
plays
a
crucial
role
in
wound
healing
and
the
host
immune
response
following
pathogenic
invasion.
However,
unresolved
chronic
inflammation
can
result
tissue
fibrosis
genetic
alterations
that
contribute
to
pathogenesis
of
human
diseases
such
as
cancer.
Recent
scientific
advancements
exploring
underlying
mechanisms
malignant
cellular
transformations
cancer
progression
have
exposed
significant
disparities
between
pediatric
adult-onset
cancers.
For
instance,
cancers
tend
lower
mutational
burdens
arise
actively
developing
tissues,
where
cell-cycle
dysregulation
leads
gene,
chromosomal,
fusion
gene
development
not
seen
counterparts.
As
such,
findings
adult
cannot
be
directly
applied
cancers,
unique
mutations
inherent
etiologies
remain
poorly
understood.
Here,
we
review
processes
chromosomal
instability,
tumor
microenvironment,
tumorigenesis
transformation
explore
current
therapeutic
interventions
maintain
and/or
restore
inflammatory
homeostasis.
Abstract
Lung
cancer
(LC)
is
a
heterogeneous
disease
consisting
mainly
of
two
subtypes,
non-small
cell
lung
(NSCLC)
and
small
(SCLC),
remains
the
leading
cause
death
worldwide.
Despite
recent
advances
in
therapies,
overall
5-year
survival
rate
LC
less
than
20%.
The
efficacy
current
therapeutic
approaches
compromised
by
inherent
or
acquired
drug-resistance
severe
off-target
effects.
Therefore,
identification
development
innovative
effective
are
critically
desired
for
LC.
RNA-mediated
gene
inhibition
technologies
was
turning
point
field
RNA
biology.
critical
regulatory
role
different
RNAs
multiple
pathways
makes
them
rich
source
targets
tools
developing
anticancer
therapies.
antisense
sequences,
short
interfering
(siRNAs),
microRNAs
(miRNAs
miRs),
anti-miRs,
mRNA-based
platforms
holds
great
promise
preclinical
early
clinical
evaluation
against
In
last
decade,
RNA-based
therapies
have
substantially
expanded
tested
trials
malignancies,
including
This
article
describes
understanding
various
aspects
therapeutics,
modern
platforms,
modifications,
combinations
with
chemo-/immunotherapies
that
translational
potential
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(6), С. 3098 - 3098
Опубликована: Март 7, 2024
Epitranscriptomic
mechanisms,
which
constitute
an
important
layer
in
post-transcriptional
gene
regulation,
are
involved
numerous
cellular
processes
under
health
and
disease
such
as
stem
cell
development
or
cancer.
Among
various
RNA
methylation
is
considered
to
have
vital
roles
eukaryotes
primarily
due
its
dynamic
reversible
nature.
There
methylations
that
include,
but
not
limited
to,
2'-O-dimethyladenosine
(m6Am),
N7-methylguanosine
(m7G),
N6-methyladenosine
(m6A)
N1-methyladenosine
(m1A).
These
biochemical
modifications
modulate
the
fate
of
by
affecting
translation,
target
site
determination,
processing,
polyadenylation,
splicing,
structure,
editing
stability.
Thus,
it
highly
quantitatively
measure
changes
marks
gain
insight
into
disease.
Although
there
complicating
challenges
identifying
certain
genome
wide,
methods
been
developed
recently
facilitate
quantitative
measurement
methylated
RNAs.
To
this
end,
detection
for
can
be
classified
five
categories
antibody-based,
digestion-based,
ligation-based,
hybridization-based
direct
RNA-based
methods.
In
review,
we
aimed
summarize
our
current
understanding
methylation,
highlighting
their
advantages
disadvantages,
along
with
field.
Cancer Cell International,
Год журнала:
2023,
Номер
23(1)
Опубликована: Окт. 16, 2023
Abstract
Epigenetic
modifications
have
long
been
recognized
as
an
essential
level
in
transcriptional
regulation
linking
behavior
and
environmental
conditions
or
stimuli
with
biological
processes
disease
development.
Among
them,
methylation
is
the
most
abundant
of
these
reversible
epigenetic
marks,
predominantly
occurring
on
DNA,
RNA,
histones.
Methylation
modification
intimately
involved
regulating
gene
transcription
cell
differentiation,
while
aberrant
status
has
linked
cancer
development
several
malignancies.
Early
detection
precise
restoration
dysregulated
form
basis
for
epigenetics-based
therapeutic
strategies.
In
this
review,
we
summarize
current
basic
understanding
mechanisms
responsible
cover
cutting-edge
research
techniques
detecting
across
genome
transcriptome.
We
then
explore
recent
advances
clinical
diagnostic
applications
markers
various
cancers
address
state
future
prospects
therapies
different
diseases,
especially
comparing
pharmacological
methylase/demethylase
inhibitors
CRISPRoff/on
editing
systems.
This
review
thus
provides
a
resource
emerging
role
cancer,
use
methylation-based
biomarkers
detection,
novel
methylation-targeted
drugs.
The
prominent
cultivation
of
lemongrass
relies
on
the
pharmacological
incentives
its
essential
oil.
oil
(LEO)
has
a
significant
amount
citral
(mixture
geranial
and
neral),
isoneral,
isogeranial,
geraniol,
geranyl
acetate,
citronellal,
citronellol,
germacrene-D,
elemol
in
addition
to
numerous
other
bioactive
compounds.
These
components
confer
various
medicinal
activities
LEO
including
antifungal,
antibacterial,
antiviral,
anticancer,
antioxidant
properties.
attributes
are
commercially
exploited
pharmaceutical,
cosmetics,
food
preservations
industries.
Furthermore,
employment
treatment
cancer
opens
new
vista
field
therapeutics.
Although
different
have
shown
promising
anticancer
vitro,
these
effects
not
been
assessed
yet
humans.
Further
studies
mechanisms
exerted
by
required.
present
review
intends
provide
timely
discussion
relevance
extracts
health
treatment,
industry
applications.
Frontiers in Immunology,
Год журнала:
2022,
Номер
13
Опубликована: Май 3, 2022
The
epigenetic
modification
of
tumorigenesis
and
progression
in
neoplasm
has
been
demonstrated
recent
studies.
Nevertheless,
the
underlying
association
N7-methylguanosine
(m7G)
regulation
with
molecular
heterogeneity
tumor
microenvironment
(TME)
clear
cell
renal
carcinoma
(ccRCC)
remains
unknown.
We
explored
expression
profiles
genetic
variation
features
m7G
regulators
identified
their
correlations
patient
outcomes
pan-cancer.
Three
distinct
patterns,
including
MGCS1,
MGCS2,
MGCS3,
were
further
determined
systematically
characterized
via
multi-omics
data
ccRCC.
Compared
other
two
subtypes,
patients
MGCS3
exhibited
a
lower
clinical
stage/grade
better
prognosis.
MGCS1
showed
lowest
enrichment
metabolic
activities.
MGCS2
was
by
suppression
immunity.
then
established
validated
scoring
tool
named
m7Sig,
which
could
predict
prognosis
ccRCC
patients.
This
study
revealed
that
played
vital
role
formation
Evaluating
landscape
helps
us
to
raise
awareness
strengthen
understanding
ccRCC's
characterization
and,
furthermore,
guide
future
decision
making.
Cells,
Год журнала:
2021,
Номер
10(1), С. 128 - 128
Опубликована: Янв. 11, 2021
Mono(ADP-ribose)
transferases
and
mono(ADP-ribosyl)ating
sirtuins
use
NAD+
to
perform
the
mono(ADP-ribosyl)ation,
a
simple
form
of
post-translational
modification
proteins
and,
in
some
cases,
nucleic
acids.
The
availability
is
limiting
step
an
essential
requisite
for
consuming
enzymes.
synthesis
degradation
NAD+,
as
well
transport
its
key
intermediates
among
cell
compartments,
play
vital
role
maintenance
optimal
levels,
which
are
regulation
NAD+-utilizing
In
this
review,
we
provide
overview
current
knowledge
metabolism,
highlighting
functional
liaison
with
enzymes,
such
well-known
ARTD10
(also
named
PARP10),
SIRT6,
SIRT7.
To
aim,
discuss
link
these
enzymes
metabolism
chronic
diseases,
cancer,
degenerative
disorders
aging.