Biosynthesis of Silver and Gold Nanoparticles and Their Efficacy Towards Antibacterial, Antibiofilm, Cytotoxicity, and Antioxidant Activities

Applied Biochemistry and Biotechnology, Год журнала: 2022, Номер 195(2), С. 1158 - 1183

Опубликована: Ноя. 7, 2022

The World Health Organization (WHO) reports that the emergence of multidrug-resistant and slow advent novel more potent antitumor antimicrobial chemotherapeutics continue to be highest concern for human health. Additionally, stability, low solubility, negative effects existing drugs make them ineffective. Studies into alternative tactics tackle such tenacious diseases was sparked by anticancer antibacterial. Silver (Ag) gold (Au) nanoparticles (NPs) were created from Trichoderma saturnisporum, much productive fungal strain. Functional extracellular enzymes proteins carried out activities synthesis capping generated nano-metals. Characterization done on obtained Ag-NPs Au-NPs through UV-vis, FTIR, XRD, TEM, SEM. versus methicillin-sensitive Staphylococcus aureus (MSSA) methicillin-resistant (MRSA), Pseudomonas aeruginosa, Klebsiella pneumoniae, antibacterial assessed. In particular, effective against pathogenic bacteria than Au-NPs. Furthermore, antibiofilm study shown had activity Ag-NPs. Interestingly, applying DPPH procedure, these noble metallic NPs antioxidant activity, in which IC50 73.5 μg/mL 190.0 μg/mL, respectively. According cytotoxicity evaluation results, alteration cells as loss their typical shape, partial or complete monolayer, granulation, shrinking, cell rounding with normal Vero 693.68 661.24 Au-NPs, While cancer (Mcf7) 370.56 394.79 produced via green have potential employed medical industry beneficial nanocompounds.

Язык: Английский

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Green synthesis of CuO nanoparticles for biological applications

Inorganic Chemistry Communications, Год журнала: 2023, Номер 155, С. 111088 - 111088

Опубликована: Июль 19, 2023

Язык: Английский

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Green synthesis of metal nanoparticles and their environmental applications

Current Opinion in Environmental Science & Health, Год журнала: 2022, Номер 29, С. 100390 - 100390

Опубликована: Авг. 28, 2022

Язык: Английский

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Nano-bioremediation: an eco-friendly and effective step towards petroleum hydrocarbon removal from environment

Environmental Research, Год журнала: 2023, Номер 231, С. 116224 - 116224

Опубликована: Май 23, 2023

Язык: Английский

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Insights into the bacterial synthesis of metal nanoparticles

Frontiers in Nanotechnology, Год журнала: 2023, Номер 5

Опубликована: Авг. 10, 2023

Metal nanoparticles have attracted considerable attention due to their astounding potential for a wide range of commercial applications. From targeted drug delivery and antimicrobial agents electronics, metal seem immeasurable prospects in all areas science. However, modern industrial production frequently involves complex procedures, large amounts energy, utilizes strong chemical solvents, or produces hazardous waste. Biological synthesis has been proposed as an alternative simpler, inexpensive, more eco-friendly nanoparticle production. Microorganisms possess multiple mechanisms transport, regulate bind ions that may result the biosynthesis nanoparticles. They can synthesize even bimetallic nanoparticles, which are difficult produce with normal physical processes. A better understanding bacteria-metal interactions might thus pave way array This review will summarize current methods synthesis, focus on microbial (bio) We describe general ion interactions, including cellular uptake subsequent reduction into Protocols metal-based relevant elements different bacterial strains compiled challenges industry discussed.

Язык: Английский

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Inhibitory Effects of Biosynthesized Copper Nanoparticles on Biofilm Formation of Proteus mirabilis

Iraqi Journal of Science, Год журнала: 2024, Номер unknown, С. 65 - 78

Опубликована: Янв. 30, 2024

This study aimed to explore whether green synthesized copper nanoparticles (CuNPs) can function as an anti-biofilm agent produced by P. mirabilis. The were from cells free extract of Characterization biosynthesized was carried out determine the chemical and physical properties product using atomic-force microscopy (AFM), transmission electron (TEM), field emission scanning (FESEM), X-ray diffraction (XRD) UV-visible spectroscopy. hexagonal structure confirmed XRD, size range marked 13-19nm TEM. FESEM used confirm surface morphology. AFM analysis reveal roughness distribution nanoparticles. UV–visible spectra recorded a maximum peak at 380 nm. Copper showed remarkable biofilm inhibitory effects on wild type strains multidrug resistant Proteus After incubation for 24 48 hours 37oC with 128 μg/ml sub minimum concentration (MIC) CuO nanoparticles, strong producer weak production. Down regulation changes in LuxS expression real time PCR technology detected after treatment these strains.

Язык: Английский

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Synthesis and Organization of Gold-Peptide Nanoparticles for Catalytic Activities

ACS Omega, Год журнала: 2022, Номер 7(2), С. 2082 - 2090

Опубликована: Янв. 6, 2022

A significant development in the synthesis strategies of metal-peptide composites and their applications biomedical bio-catalysis has been reported. However, random aggregation gold nanoparticles provides opportunity to find alternative fabrication gold-peptide composite nanomaterials. In this study, we used a facile strategy synthesize via green simple approach where they show self-alignment on assembled nanofibers ultrashort oligopeptides as material. photochemical reduction method is used, which does not require any external chemical reagents for ions, resultantly makes size ca. 5 nm under mild UV light exposure. The specific arrangement peptide may indicate electrostatic interactions two components with amino group building block. Furthermore, nanoparticle ability catalyst degradation environmental pollutant p-nitrophenol p-aminophenol, reaction rate constant catalysis calculated 0.057 min–1 at 50-fold dilute sample 2 mg/mL 0.72 mM concentration composites. This colloidal would help researchers fabricate metalized bioorganic various applications.

Язык: Английский

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Phytochemical Analysis, α-Glucosidase and Amylase Inhibitory, and Molecular Docking Studies on Persicaria hydropiper L. Leaves Essential Oils

Evidence-based Complementary and Alternative Medicine, Год журнала: 2022, Номер 2022, С. 1 - 11

Опубликована: Янв. 19, 2022

Medicinal plants and essentials oils are well known for diverse biological activities including antidiabetic potential. This study was designed to isolate essential from the leaves of Persicaria hydropiper L. (P. hydropiper), perform its phytochemical analysis, explore in vitro effects.P. (Ph.Los) were extracted using a hydrodistillation apparatus subjected analysis gas chromatography mass spectrometry (GC-MS) technique. Ph.Lo tested against two vital enzymes α-glucosidase α-amylase which important targets type-2 diabetes. The identified compounds silico approaches their binding affinities enzyme MOE-Dock software.GC-MS revealed presence 141 among dihydro-alpha-ionone, cis-geranylacetone, α-bulnesene, nerolidol, β-caryophyllene epoxide, decahydronaphthalene most abundant compounds. exhibited considerable inhibitory potential with 70% inhibition at 1000 μg mL-1 highest concentration. activity positive control acarbose 77.30 ± 0.61% same IC50 170 18 µg correspondingly. Furthermore, dose-dependent inhibitions observed an 890 mL-1. top-ranked docking conformation epoxide score -8.3182 α-glucosidase, it has established seven hydrogen bonds one H-pi interaction active site residues (Phe 177, Glu 276, Arg 312, Asp 349, Gln 350, 408, 439). Majority fit pocket Tyr 62, 197, 233, 300, His 305, Ala 307 α-amylase. β-Caryophyllene found be inhibitor -8.3050 formed five 300 making polar interactions ligand.The current that is rich bioactive metabolites might contribute Inhibition these key target reducing postprandial hyperglycemia. However, further detailed vivo studies required therapeutic activities.

Язык: Английский

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Neuroprotective potentials of Lead phytochemicals against Alzheimer's disease with focus on oxidative stress-mediated signaling pathways: Pharmacokinetic challenges, target specificity, clinical trials and future perspectives

Phytomedicine, Год журнала: 2023, Номер 124, С. 155272 - 155272

Опубликована: Дек. 12, 2023

Язык: Английский

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Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

PLoS ONE, Год журнала: 2023, Номер 18(4), С. e0280553 - e0280553

Опубликована: Апрель 4, 2023

Green synthesis of nanoparticles is becoming a method choice for biological research due to its environmentally benign outcomes, stability and ease synthesis. In this study, silver (AgNPs) were synthesized using stem (S-AgNPs), root (R-AgNPs) mixture (RS-AgNPs) Delphinium uncinatum. The characterized by standardized techniques evaluated their antioxidant, enzyme inhibition, cytotoxic antimicrobial potentials. AgNPs exhibited efficient antioxidant activities considerable inhibition potential against alpha amylase, acetylcholinesterase (AChE) butyrylcholinesterase (BChE) enzymes. S-AgNPs showed strong cytotoxicity human hepato-cellular carcinoma cells (HepG2) high inhibitory effect (IC50 values 27.5μg/ml AChE 22.60 μg/ml BChE) compared R-AgNPs RS-AgNPs. RS-AgNPs significant Klebsiella pneumoniae Aspergillus flavus higher biocompatibility (<2% hemolysis) in red blood hemolytic assays. present study that biologically the extract various parts D. uncinatum have

Язык: Английский

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