Analytical Chemistry,
Год журнала:
2023,
Номер
95(41), С. 15153 - 15161
Опубликована: Окт. 6, 2023
The
metabolic
cross-talk
between
tumor
and
immune
cells
plays
key
roles
in
cell
function
checkpoint
blockade
therapy.
However,
the
characterization
of
immunometabolism
its
spatiotemporal
alterations
during
response
a
complex
microenvironment
is
challenging.
Here,
3D
tumor-immune
coculture
spheroid
model
was
developed
to
mimic
interactions,
combined
with
mass
spectrometry
imaging-based
spatially
resolved
metabolomics
visualize
immunometabolic
response.
inhibition
T
simulated
by
coculturing
breast
spheroids
Jurkat
cells,
reactivation
can
be
monitored
through
diminishing
cancer
PD-L1
expressions
berberine.
This
system
enables
simultaneously
screening
imaging
discriminatory
metabolites
that
are
altered
cell-mediated
antitumor
characterizing
distributions
berberine
spheroids.
We
discovered
transport
catabolism
glutamine
were
significantly
reprogrammed
at
both
metabolite
enzyme
levels,
corresponding
indispensable
energy
metabolism
building
new
biomass.
combination
visually
reveals
interactions
possibly
helps
decipher
role
immunotherapy.
Frontiers in Nutrition,
Год журнала:
2023,
Номер
10
Опубликована: Фев. 16, 2023
Metabolic
reprogramming
is
one
of
fourteen
hallmarks
tumor
cells,
among
which
aerobic
glycolysis,
often
known
as
the
“Warburg
effect,”
essential
to
fast
proliferation
and
aggressive
metastasis
cells.
Lactate,
on
other
hand,
a
ubiquitous
molecule
in
microenvironment
(TME),
generated
primarily
by
cells
undergoing
glycolysis.
To
prevent
intracellular
acidification,
malignant
remove
lactate
along
with
H
+
,
yet
acidification
TME
inevitable.
Not
only
does
highly
concentrated
within
serve
substrate
supply
energy
but
it
also
works
signal
activate
multiple
pathways
that
enhance
invasion,
intratumoral
angiogenesis,
well
immune
escape.
In
this
review,
we
aim
discuss
latest
findings
metabolism
particularly
capacity
extracellular
influence
microenvironment.
addition,
examine
current
treatment
techniques
employing
existing
medications
target
interfere
generation
transport
cancer
therapy.
New
research
shows
targeting
metabolism,
lactate-regulated
action
are
viable
therapy
strategies.
Abstract
Breast
cancer,
the
most
frequent
female
malignancy,
is
often
curable
when
detected
at
an
early
stage.
The
treatment
of
metastatic
breast
cancer
more
challenging
and
may
be
unresponsive
to
conventional
therapy.
Immunotherapy
crucial
for
treating
but
its
resistance
a
major
limitation.
tumor
microenvironment
(TME)
vital
in
modulating
immunotherapy
response.
Various
microenvironmental
components,
such
as
cancer-associated
fibroblasts
(CAFs),
tumor-associated
macrophages
(TAMs),
myeloid-derived
suppressor
cells
(MDSCs),
are
involved
TME
modulation
cause
resistance.
This
review
highlights
role
stromal
microenvironment,
including
involvement
CAF-TAM
interaction,
alteration
metabolism
leading
failure,
other
latest
strategies,
high
throughput
genomic
screening,
single-cell
spatial
omics
techniques
identifying
immune
genes
regulating
emphasizes
therapeutic
approach
overcome
through
CAF
reprogramming,
TAM
polarization,
metabolism,
alterations.
Abstract
RNA
methylation,
a
prevalent
post-transcriptional
modification,
has
garnered
considerable
attention
in
research
circles.
It
exerts
regulatory
control
over
diverse
biological
functions
by
modulating
splicing,
translation,
transport,
and
stability.
Notably,
studies
have
illuminated
the
substantial
impact
of
methylation
on
tumor
immunity.
The
primary
types
encompass
N6-methyladenosine
(m6A),
5-methylcytosine
(m5C),
N1-methyladenosine
(m1A),
N7-methylguanosine
(m7G),
3-methylcytidine
(m3C).
Compelling
evidence
underscores
involvement
regulating
microenvironment
(TME).
By
affecting
translation
stability
through
"writers",
"erasers"
"readers",
influence
dysregulation
immune
cells
factors.
Consequently,
plays
pivotal
role
immunity
mediating
various
behaviors,
encompassing
proliferation,
invasion,
metastasis,
etc.
In
this
review,
we
discussed
mechanisms
several
methylations,
providing
comprehensive
overview
their
roles
underlying
within
among
immunocytes.
exploring
how
these
modifications
mediate
evasion,
also
examine
potential
applications
immunotherapy.
This
review
aims
to
provide
novel
insights
strategies
for
identifying
targets
advancing
cancer
immunotherapy
efficacy.
Signal Transduction and Targeted Therapy,
Год журнала:
2025,
Номер
10(1)
Опубликована: Фев. 20, 2025
The
progression
of
malignant
tumors
leads
to
the
development
secondary
in
various
organs,
including
bones,
brain,
liver,
and
lungs.
This
metastatic
process
severely
impacts
prognosis
patients,
significantly
affecting
their
quality
life
survival
rates.
Research
efforts
have
consistently
focused
on
intricate
mechanisms
underlying
this
corresponding
clinical
management
strategies.
Consequently,
a
comprehensive
understanding
biological
foundations
tumor
metastasis,
identification
pivotal
signaling
pathways,
systematic
evaluation
existing
emerging
therapeutic
strategies
are
paramount
enhancing
overall
diagnostic
treatment
capabilities
for
tumors.
However,
current
research
is
primarily
metastasis
within
specific
cancer
types,
leaving
significant
gaps
our
complex
cascade,
organ-specific
tropism
mechanisms,
targeted
treatments.
In
study,
we
examine
sequential
processes
elucidate
driving
organ-tropic
systematically
analyze
tumors,
those
tailored
organ
involvement.
Subsequently,
synthesize
most
recent
advances
technologies
challenges
opportunities
encountered
pertaining
bone
metastasis.
Our
objective
offer
insights
that
can
inform
future
practice
crucial
field.
Nature Metabolism,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 5, 2025
Preclinical
studies
have
shown
that
asparagine
deprivation
enhances
T
cell
antitumour
responses.
Here
we
apply
compassionate
use
of
L-asparaginase,
usually
employed
to
treat
blood
malignancies,
on
patients
with
recurrent
metastatic
nasopharyngeal
carcinoma.
The
L-asparaginase
notably
immune-checkpoint
blockade
therapy
in
by
strengthening
CD8+T
fitness.
Our
study
shows
this
combination
is
a
promising
avenue
for
clinical
application
and
provides
further
mechanistic
insight
into
how
restriction
rewires
metabolism.
Journal of Hematology & Oncology,
Год журнала:
2022,
Номер
15(1)
Опубликована: Июль 6, 2022
Abstract
The
tumor
microenvironment
(TME),
which
is
regulated
by
intrinsic
oncogenic
mechanisms
and
epigenetic
modifications,
has
become
a
research
hotspot
in
recent
years.
Characteristic
features
of
TME
include
hypoxia,
metabolic
dysregulation,
immunosuppression.
One
the
most
common
RNA
N6-methyladenosine
(m
6
A)
methylation,
widely
involved
regulation
physiological
pathological
processes,
including
development.
Compelling
evidence
indicates
that
m
A
methylation
regulates
transcription
protein
expression
through
shearing,
export,
translation,
processing,
thereby
participating
dynamic
evolution
TME.
Specifically,
methylation-mediated
adaptation
to
phenotypic
shift
immune
cells
synergistically
promote
formation
an
immunosuppressive
supports
proliferation
metastasis.
In
this
review,
we
have
focused
on
involvement
tumor-adaptive
described
detailed
linking
change
cell
biological
functions.
view
collective
data,
advocate
treating
as
complete
ecosystem
components
crosstalk
with
each
other
achieve
adaptive
changes.
Finally,
describe
potential
utility
methylation-targeted
therapies
immunotherapy
clinical
applications
challenges
faced,
aim
advancing
research.
The Innovation,
Год журнала:
2023,
Номер
4(2), С. 100391 - 100391
Опубликована: Фев. 9, 2023
Echinacea
purpurea
modulates
tumor
progression,
but
the
underlying
mechanism
is
poorly
defined.
We
isolated
and
purified
a
novel
homogeneous
polysaccharide
from
E.
(EPPA),
which
was
shown
to
be
an
arabinogalactan
with
mean
molecular
mass
(Mr)
of
3.8
×
104
Da
α-
(1
→
5)
-L-Arabinan
as
backbone
α-L-Araf-(1→,
→6)-β-D-Galp-(1→,
→4)-α-D-GalpA-(1→
side
chains.
Interestingly,
oral
administration
EPPA
suppresses
progression
in
vivo
shapes
immune
cell
profile
(e.g.,
facilitating
M1
macrophages)
microenvironment
by
single-cell
RNA
sequencing
(scRNA-seq)
analysis.
More
importantly,
activates
inflammasome
through
phagocytosis-dependent
rewires
transcriptomic
metabolic
profile,
thereby
potentiating
macrophage
polarization.
Collectively,
we
propose
that
supplementation
could
function
adjuvant
therapeutic
strategy
for
suppression.
