The development of a fluorescence/colorimetric biosensor based on the cleavage activity of CRISPR-Cas12a for the detection of non-nucleic acid targets

Journal of Hazardous Materials, Год журнала: 2023, Номер 449, С. 131044 - 131044

Опубликована: Фев. 20, 2023

Язык: Английский

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A label-free electrochemical sensor for the detection of two kinds of targets based on CRISPR/Cas12a system

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 406, С. 135406 - 135406

Опубликована: Янв. 28, 2024

Язык: Английский

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Reduced graphene oxide-based silver selenide nanocomposite synthesized through hydrothermal method for oxygen evolution reaction

Journal of Alloys and Compounds, Год журнала: 2024, Номер 993, С. 174626 - 174626

Опубликована: Апрель 24, 2024

Язык: Английский

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Multiplexed detection of foodborne pathogens using one-pot CRISPR/Cas12a combined with recombinase aided amplification on a finger-actuated microfluidic biosensor

Biosensors and Bioelectronics, Год журнала: 2022, Номер 220, С. 114885 - 114885

Опубликована: Ноя. 5, 2022

Язык: Английский

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Recent advances and applications of Bi2S3-based composites in photoelectrochemical sensors and biosensors

TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 158, С. 116876 - 116876

Опубликована: Дек. 6, 2022

Язык: Английский

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Integrated solar-powered MEMS-based photoelectrochemical immunoassay for point-of-care testing of cTnI protein

Biosensors and Bioelectronics, Год журнала: 2022, Номер 223, С. 115028 - 115028

Опубликована: Дек. 22, 2022

Язык: Английский

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Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21

Analytical Chemistry, Год журнала: 2023, Номер unknown

Опубликована: Янв. 5, 2023

Sensitive and specific assay of microRNAs (miRNAs) is beneficial to early disease screening. Herein, we for the first time proposed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a-mediated photoelectrochemical biosensors direct miRNA-21. In this study, compared with traditional nucleic acid-based signal amplification strategies, CRISPR/Cas13a system can greatly improve specificity sensitivity target determination due its accurate recognition high-efficient trans-cleavage capability without complex acid sequence design. Moreover, CRISPR/Cas12a-based biosensing platform, developed CRISPR/Cas13a-mediated biosensor directly detect RNA targets transduction from DNA, thereby avoiding leakage distortion. Generally, reveals excellent analysis a wider linear range 1 fM 5 nM lower detection limit fM. Additionally, it also shows satisfactory stability in human serum samples cell lysates, manifesting that has great application prospects areas diagnosis biomedical research.

Язык: Английский

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Synergistic Incorporation of Two ssDNA Activators Enhances the Trans-Cleavage of CRISPR/Cas12a

Analytical Chemistry, Год журнала: 2023, Номер 95(23), С. 8879 - 8888

Опубликована: Май 30, 2023

CRISPR/Cas12a has been believed to be powerful in molecular detection and diagnostics due its amplified trans-cleavage feature. However, the activating specificity multiple activation mechanisms of Cas12a system are yet elucidated fully. Herein, a "synergistic activator effect" is discovered, which supports an mechanism that synergistic incorporation two short ssDNA activators can promote CRISPR/Cas12a, while either them too work independently. As proof-of-concept example, activator-triggered successfully harnessed AND logic operation discrimination single-nucleotide variants, requiring no signal conversion elements or other enzymes. Moreover, achieved for variants by pre-introducing synthetic mismatch between crRNA "helper" activator. The finding not only provides deeper insight into but also may facilitate expanded application power exploration undiscovered properties CRISPR/Cas systems.

Язык: Английский

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Bio-inspired polynorepinephrine based nanocoatings for reduced graphene oxide/gold nanoparticles composite for high-performance biosensing of Mycobacterium tuberculosis

Environmental Research, Год журнала: 2023, Номер 227, С. 115684 - 115684

Опубликована: Март 13, 2023

Язык: Английский

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A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

Biosensors and Bioelectronics, Год журнала: 2023, Номер 225, С. 115102 - 115102

Опубликована: Янв. 25, 2023

Язык: Английский

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