Analytical Methods, Год журнала: 2024, Номер 16(6), С. 798 - 816
Опубликована: Янв. 1, 2024
The main types of CRISPR/Cas systems based on optical biosensors.
Язык: Английский
Journal of Hazardous Materials, Год журнала: 2023, Номер 452, С. 131195 - 131195
Опубликована: Март 11, 2023
Язык: Английский
Процитировано
62
Talanta, Год журнала: 2024, Номер 275, С. 126080 - 126080
Опубликована: Апрель 10, 2024
Язык: Английский
Процитировано
30
Trends in Food Science & Technology, Год журнала: 2024, Номер 154, С. 104771 - 104771
Опубликована: Ноя. 2, 2024
Язык: Английский
Процитировано
19
Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(37), С. 13577 - 13594
Опубликована: Сен. 1, 2023
Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.
Язык: Английский
Процитировано
34
TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 167, С. 117269 - 117269
Опубликована: Сен. 7, 2023
Язык: Английский
Процитировано
31
Critical Reviews in Food Science and Nutrition, Год журнала: 2023, Номер 64(32), С. 12061 - 12082
Опубликована: Сен. 10, 2023
Food safety problems have become one of the most important public health issues worldwide. Therefore, development rapid, effective and robust detection is great importance. Amongst a range methods, nucleic acid isothermal amplification (NAIA) plays role in food detection. However, widespread application remains limited due to few shortcomings. CRISPR/Cas system has emerged as powerful tool detection, which could be readily integrated with NAIA improve sensitivity, specificity, adaptability versatility dependability. currently there was lack comprehensive summary regarding integration field In this review, recent advances based on CRISPR/Cas-integrated were comprehensively reviewed. To begin with, summarized. Then, types working principles introduced. The applications for mainly introduced objectively discussed. Lastly, current challenges future opportunities proposed. summary, technology expected an approach leading safer more reliable industry.
Язык: Английский
Процитировано
24
Small, Год журнала: 2024, Номер 20(29)
Опубликована: Фев. 28, 2024
Rare earth-doped upconversion nanoparticles (UCNPs) have achieved a wide range of applications in the sensing field due to their unique anti-Stokes luminescence property, minimized background interference, excellent biocompatibility, and stable physicochemical properties. However, UCNPs-based platforms still face several challenges, including inherent limitations from UCNPs such as low quantum yields narrow absorption cross-sections, well constraints related energy transfer efficiencies systems. Therefore, construction high-performance is an important cornerstone for conducting relevant research. This work begins by providing brief overview mechanism UCNPs. Subsequently, it offers comprehensive summary sensors' types, design principles, optimized strategies platforms. More cost-effective promising point-of-care testing implemented based on systems are also summarized. Finally, this addresses future challenges prospects
Язык: Английский
Процитировано
17
ACS Sensors, Год журнала: 2024, Номер 9(7), С. 3511 - 3519
Опубликована: Апрель 23, 2024
Excavating nucleic acid quantitative capabilities by combining clustered regularly interspaced short palindromic repeats (CRISPR) and isothermal amplification in one pot is of common interest. However, the mutual interference between CRISPR cleavage primary obstacle to detection. Though several works have demonstrated enhanced detection sensitivity reducing inhibition on pot, few paid attention process even dynamic reaction processes two. Herein, we find that DNA quantification can be realized regulating either recombinase polymerase (RPA) efficiency or CRISPR/Cas12a cleaving (namely, tuning balance) pot. The sensitive utilizing dual PAM-free crRNAs for recognition. varied RPA primer concentration with stabilized systems significantly affects performances. Alternatively, also achieved stabilizing while concentration. capability proved detecting targets from Lactobacillus acetotolerans SARS-CoV-2. performance toward real samples comparable real-time PCR L. spiked fermented food SARS-CoV-2 clinical samples. We expect presented method will a powerful tool quantifying other targets.
Язык: Английский
Процитировано
15
Clinica Chimica Acta, Год журнала: 2025, Номер 569, С. 120179 - 120179
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
2
ACS Sensors, Год журнала: 2023, Номер 8(11), С. 3988 - 4007
Опубликована: Окт. 23, 2023
Point-of-care (POC) detection is getting more and attention in many fields due to its accuracy on-site test property. The CRISPR/Cas12a system endowed with excellent sensitivity, target identification specificity, signal amplification ability biosensing because of unique trans-cleavage ability. As a result, lot research has been made develop CRISPR/Cas12a-based biosensors. In this review, we focused on readout strategies summarized recent sensitivity-improving fluorescence, colorimetric, electrochemical signaling. Then introduced novel portability-improving based lateral flow assays (LFAs), microfluidic chips, simplified instruments, one-pot design. the end, also provide our outlook for future development
Язык: Английский
Процитировано
22