Analytical Chemistry, Год журнала: 2024, Номер 96(21), С. 8665 - 8673
Опубликована: Май 9, 2024
Prostate-specific antigen (PSA) is a key marker for prostate cancer diagnosis. The low sensitivity of traditional lateral flow immunoassay (LFIA) methods makes them unsuitable point-of-care testing. Herein, we designed nanozyme by in situ growth Prussian blue (PB) within the pores dendritic mesoporous silica (DMSN). PB was forcibly dispersed into DMSN, leading to an increase exposed active sites. Consequently, atom utilization enhanced, resulting superior peroxidase (POD)-like activity compared that cubic PB. Antibody-modified DMSN@PB nanozymes serve as immunological probes enzymatic-enhanced colorimetric and photothermal dual-signal LFIA PSA detection. After systematic optimization, based on successfully achieves 4-fold amplification signal 7 min through catalytic oxidation chromogenic substrate POD-like activity. Moreover, exhibits excellent conversion ability under 808 nm laser irradiation. Accordingly, signals are introduced improve anti-interference LFIA, exhibiting wide linear range (1–40 ng mL–1) detection limit (0.202 mL–1), which satisfies early level cancer. This research provides more accurate reliable visualization analysis methodology diagnosis
Язык: Английский
Процитировано
14
Microchemical Journal, Год журнала: 2024, Номер 201, С. 110487 - 110487
Опубликована: Апрель 4, 2024
Язык: Английский
Процитировано
10
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 176, С. 117742 - 117742
Опубликована: Май 2, 2024
Язык: Английский
Процитировано
10
Biosensors, Год журнала: 2023, Номер 13(4), С. 469 - 469
Опубликована: Апрель 11, 2023
Transistor-based biochemical sensors feature easy integration with electronic circuits and non-invasive real-time detection. They have been widely used in intelligent wearable devices, skins, biological analyses shown broad application prospects medical Field-effect transistor (FET) high sensitivity, reasonable specificity, rapid response, portability provide unique signal amplification during Organic field-effect (OFET) are lightweight, flexible, foldable, biocompatible devices. electrochemical (OECT) convert signals body fluids into electrical for artificial intelligence analysis. In addition to markers fluids, electrophysiology indicators such as electrocardiogram (ECG) temperature can also cause changes the current or voltage of transistor-based sensors. When modified sensitive substances, detect specific analytes, improve broaden detection range, reduce limit (LoD). this review, we introduce three kinds sensors: FET, OFET, OECT. We discuss fabrication processes sources, drains, gates. Furthermore, demonstrated sensor types fluid biomarkers, signals, development trends. exhibit excellent potential multi-mode analysis good candidates next generation point-of-care testing (iPOCT).
Язык: Английский
Процитировано
22
Analytical Chemistry, Год журнала: 2024, Номер 96(24), С. 9909 - 9916
Опубликована: Июнь 3, 2024
The development of the Point-of-Care Testing (POCT) platform that combines convenience and cost-effectiveness is crucial for enabling visual detection disease biomarkers. In this work, a POCT sensitive in situ prostate specific antigen (PSA) with dual-signal output was constructed by functionalizing Eppendorf (EP) tube. This achieved through modification aptamer hairpin probes (AHPs) on lid EP tube assembly nanoenzyme hydrogel film its inner wall. target could trigger release Ag+ AHP subsequently activate Ag+-dependent DNAzyme (Ag-DNAzyme). would initiate cleavage DNA-Au/Pt NP network, leading to Au/Pt NPs. released NPs exhibit both peroxidase (POD)-like catalase (CAT)-like activity produce colorimetric response induce liquid flow under pressure. Therefore, can be measured visually quantitatively analysis measurement total dissolved solids (TDS) using pressure-triggered device integrated into platform. designed distinguished simplicity, specificity, cost-effectiveness, remarkable sensitivity. It allows PSA within concentration ranges 0.5–100 ng/L (colorimetric) 3–100 (TDS reading), boasting limits as low 0.15 0.57 reading). strategy target-triggered significantly enhances sensitivity provides guiding approach biomarker detection.
Язык: Английский
Процитировано
9
Surfaces and Interfaces, Год журнала: 2025, Номер unknown, С. 106047 - 106047
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
1
Biosensors, Год журнала: 2023, Номер 13(11), С. 987 - 987
Опубликована: Ноя. 17, 2023
Alzheimer’s disease (AD) is closely related to neurodegeneration, leading dementia and cognitive impairment, especially in people aged > 65 years old. The detection of biomarkers plays a pivotal role the diagnosis treatment AD, particularly at onset stage. Field-effect transistor (FET)-based sensors are emerging devices that have drawn considerable attention due their crucial ability recognize various ultra-low concentrations. Thus, FET broadly manipulated for AD biomarker detection. In this review, an overview typical features operational mechanisms described detail. addition, summary applicability biosensors research field outlined discussed. Furthermore, trends future prospects diagnostic applications also
Язык: Английский
Процитировано
14
Advanced Functional Materials, Год журнала: 2024, Номер unknown
Опубликована: Сен. 6, 2024
Abstract 2D metal/semiconducting heterostructures have attracted extensive attention for potential applications in future electronic and optoelectronic devices. However, the simple fast preparation of patterned with controllable channel lengths still faces challenges. Here, a reliable laser patterning method preparing lateral/vertical 1T/2H VS 2 /MoS is reported. Specifically, site‐selective etching can be realized through combination radiation acid solution etching. Further, pre‐patterned nanoplates edge dangling bonds offer effective nucleation points lateral epitaxial growth MoS , thus generating ‐MoS heterostructures. The processing further used to create vertical van der Waals (vdWHs), which only selectively etch upper layer while maintaining intrinsic structure bottom . obtained vdWHs show similar length ≈420 nm, vdW contact transistor fabricated, delivering an On‐state current 4.01 µA/µm, carrier mobility 3.56 cm s −1 V This approach also general VSe /WSe
Язык: Английский
Процитировано
6
Advanced Electronic Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 5, 2024
Abstract Biosensors based on field effect transistor (FET), also known as Bio‐FETs, offer numerous advantages, including high sensitivity, rapid response speed, low operating voltage, compact size, reliability, and easily integrative. These features endow Bio‐FETs with tremendous performance in analysis for samples ultra‐low concentration at large‐scale, broadening their multiple potential applications. In recent years, the research has attracted more attention. A lot of effort is put into challenge to improve which can be influenced by various factors. This review focuses current approaches from perspectives, overcoming influence Debye shielding effects, promoting binding efficiency analytes FETs surface, improving structures device. Correspondingly, novel strategies increase length, facilitate frequency electric modulation, enhance specific interaction efficiency, enlarge surface area (SBET) are summarized. Additionally, there a lack reliable standard method characterize sensitivity currently. Regarding this issue, most commonly used definitions representation parameters evaluated. Overall, outlines future directions insights enhancing Bio‐FETs.
Язык: Английский
Процитировано
6
RSC Advances, Год журнала: 2025, Номер 15(10), С. 8019 - 8052
Опубликована: Янв. 1, 2025
Cancer, an insidious affliction, continues to exact a heavy toll on humanity, necessitating early detection and nuanced comprehension of its intricacies for effective treatment.
Язык: Английский
Процитировано
0