ACS Applied Nano Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 30, 2024
The
development
of
sensitive
and
accurate
glucose
biosensors
is
significant
in
diabetic
patient
monitoring.
Here,
we
synthesize
carbon-decorated
Fe3O4
nanotubes
(Fe3O4@C
NTs)
using
a
rational
designed
template
method.
Through
catalytic
oxidation
typical
colorimetric
reaction,
the
prepared
Fe3O4@C
NTs
showed
significantly
better
peroxidase-like
oxidase-like
performances
than
other
similar
Fe3O4-based
nanomaterials.
Moreover,
optimal
pH,
temperature,
concentration
for
were
4.0,
60
°C,
40
μg/mL,
respectively.
We
additionally
investigated
steady-state
kinetics
NTs,
their
catalyzed
reactions
found
to
follow
Michaelis–Menten
model.
Based
on
activity
they
employed
label-free,
rapid,
selective,
highly
detection
H2O2
glucose,
demonstrating
potential
application
determination.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
The
misfolding
of
amyloid
β
(Aβ)
peptides
into
an
aggregation
state
is
a
central
hallmark
the
onset
Alzheimer's
disease
(AD).
However,
conventional
methods
are
mainly
focused
on
detecting
specific
Aβ
peptide,
which
makes
it
difficult
to
recognize
multiple
analytes
with
different
topological
features
and
unfolded
states
at
same
time.
Here,
we
propose
simple
universal
sensing
strategy
construct
fluorescence
sensor
array
by
using
single-nanozyme
probe
combined
three
fluorescent
substrates
as
recognition
units
protein
structural
changes
identify
between
assemblies.
In
this
system,
fingerprint-like
patterns
produced
from
nonspecific
interactions
proteins
units.
As
result,
can
accurately
13
kinds
their
mixtures
ratios.
Moreover,
discriminate
against
diverse
conformational
forms.
Most
importantly,
successfully
distinguishes
species,
even
in
artificial
cerebrospinal
fluid
samples
human
serum
samples.
This
work
provides
attractive
reliable
for
predicting
pathologically
relevant
clinical
diagnosis
AD.
RSC Advances,
Год журнала:
2023,
Номер
13(51), С. 36280 - 36292
Опубликована: Янв. 1, 2023
The
level
of
free
bilirubin
is
a
considerable
index
for
the
characterization
jaundice-related
diseases.
Herein,
biosensor
was
fabricated
via
immobilization
oxidase
(BOx)
on
graphene
oxide
(GO)
and
polyaniline
(PANI)
that
were
electrochemically
co-precipitated
indium
tin
(ITO)
conductive
glass.
structural
enzyme
electrode
characterized
by
FTIR,
XRD,
Raman
spectroscopy,
while
spectral
thermal
properties
investigated
UV-vis
thermogravimetric
analysis
(TGA).
Owing
to
activity
BOx/GO@PANI/ITO
biosensor,
it
could
detect
with
good
selectivity
sensitivity
in
low
response
time.
electrochemical
studied
using
impedance
spectroscopy
(EIS)
cyclic
voltammetry
(CV).
At
polarization
potential
0.2
V
vs.
Ag/AgCl,
sensor
illustrated
only
2
s
at
30
°C
pH
7.5.
LOD
LOQ
calculated
found
be
0.15
nM
2.8
nM,
respectively.
signal
showed
linear
concentration
range
0.01-250
μM.
5
°C,
demonstrated
half-time
120
days,
through
which
utilized
100
times
this
temperature
conditions.
By
common
colorimetric
method,
data
levels
serum
determination
coefficient
(R2)
0.97.
Current Research in Biotechnology,
Год журнала:
2024,
Номер
7, С. 100205 - 100205
Опубликована: Янв. 1, 2024
Nanozymes
are
a
class
of
nanoparticles
that
can
mimic
enzyme
activity
and
be
used
for
various
applications
in
modern
clinical
therapy.
It
has
recently
been
observed
nanozymes
have
multi-enzyme
mimicking
activities,
highly
stable,
versatile
easily
modified.
Moreover,
they
high
catalytic
efficiency,
recovery
rates,
improved
substrate
specificity
suitable
mass
production.
The
mechanisms
mainly
include
catalase,
peroxidase,
oxidase,
hydrolase
superoxide
dismutase-like
which
enable
to
as
potential
therapeutics
against
plethora
infectious
lifestyle
disorders.
cancer,
inflammatory
diseases,
neurodegenerative
neurological
disorders,
bacterial,
fungal
viral
infections,
wounds
diseases
associated
with
Reactive
Oxygen
Species.
purpose
writing
this
review
is
provide
comprehensive
compilation
novel
research
work
taken
place
the
last
few
years
regarding
use
We
compiled
kinds
elaborated
on
their
anti-tumorigenic,
antioxidant,
anti-inflammatory,
antibacterial,
antifungal,
antiviral,
neuroprotective
roles.
Their
modes
action
enzymatic
targets
also
discussed.
types
synthesis
summarized,
along
interactions
nanozymes.
Furthermore,
strategies
enhance
compatibility
between
analyzed.
Major
focus
laid
therapeutic
challenges
future
perspectives
using
therapy
debated
later
sections.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(6), С. 6849 - 6858
Опубликована: Янв. 31, 2024
Rationally
designing
highly
catalytic
and
stable
nanozymes
for
metabolite
monitoring
is
of
great
importance
because
their
huge
potential
in
early
disease
diagnosis.
Herein,
a
novel
nanozyme
based
on
hierarchically
structured
CuS/ZnS
with
efficient
peroxidase
(POD)-mimic
capability
was
developed
synthesized
multiple
determination
recognition
via
the
plasmon-stimulated
biosensor
array
strategy.
The
designed
can
simultaneously
harvest
plasmon
triggered
hot
electron–hole
pairs
generate
photothermal
properties,
leading
to
sharply
boosted
POD-mimic
under
808
nm
laser
irradiation.
Interestingly,
interaction
diversity
POD-like
nanomaterials,
unique
inhibitory
effect
metabolites
activity
could
be
signal
response
as
differentiation.
Thus,
utilizing
TMB
typical
chromogenic
substrate
addition
H2O2,
colorimetric
produce
diverse
fingerprints
three
vital
metabolisms
(cysteine
(Cys),
ascorbic
acid
(AA),
glutathione
(GSH)),
which
precisely
identified
by
principal
component
analysis
(PCA).
Notably,
distinct
fingerprint
single
different
levels
mixtures
also
achieved
detection
limit
1
μM.
Most
importantly,
cell
lysis
effectively
discriminated
assay,
implying
its
clinical
