Extra-embryonic mesoderm during development and in in vitro models
Development,
Год журнала:
2025,
Номер
152(5)
Опубликована: Март 1, 2025
ABSTRACT
Extra-embryonic
tissues
provide
protection
and
nutrition
in
vertebrates,
as
well
a
connection
to
the
maternal
mammals.
The
extra-embryonic
mesoderm
is
an
essential
understudied
germ
layer
present
amniotes.
It
involved
hematopoiesis,
formation
of
structures
such
amnion,
umbilical
cord
placenta.
origin
specification
are
not
entirely
conserved
across
species,
molecular
mechanisms
governing
its
function
fully
understood.
This
Review
begins
with
overview
embryonic
vertebrates
from
vivo
studies.
We
then
compare
vitro
models
that
generate
mesoderm-like
cells.
Finally,
we
discuss
how
insights
studying
both
embryos
systems
can
aid
designing
even
more
advanced
stem
cell-based
embryo
models.
Язык: Английский
Stem cell-based models of early human development
Development,
Год журнала:
2025,
Номер
152(8)
Опубликована: Апрель 15, 2025
ABSTRACT
Stem
cell-based
embryo
models
(SCBEMs)
are
structures
generated
from
three-dimensional
(3D)
culture
of
pluripotent
stem
cells
and
their
derivatives,
utilizing
mechanical
and/or
chemical
cues
to
facilitate
lineage
differentiation,
self-organization
morphogenesis.
These
partially
mimic
early
embryos,
which
would
otherwise
be
difficult
access.
SCBEMs
have
been
established
in
mice,
livestock,
nonhuman
primates
humans.
Here,
we
focus
on
recently
developed
human
models,
with
an
emphasis
the
peri-implantation
stage
aspects
development
these
recapitulate.
Язык: Английский
Signaling pathway regulators in preimplantation embryos
Journal of Molecular Histology,
Год журнала:
2024,
Номер
56(1)
Опубликована: Дек. 27, 2024
Язык: Английский
Building a molecular reference map of the human embryo
Nature Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 14, 2024
Язык: Английский
Epigenetic restoration of differentiation competency via reversal of epiblast regionalisation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 28, 2024
Abstract
Although
the
epiblast
in
embryo
has
capacity
to
generate
all
tissues
of
body,
its
vitro
counterparts
often
exhibit
differentiation
biases,
posing
significant
challenges
for
both
basic
research
and
translational
applications
involving
pluripotent
stem
cells
(PSCs).
The
origins
these
biases
remain
incompletely
understood.
In
this
study,
we
identify
PSC
as
arising
from
fluctuations
repressive
activating
histone
posttranslational
modifications,
leading
acquisition
a
caudal
epiblast-like
phenotype.
We
present
novel
approach
overcome
bias
using
chemical
chromatin
restoration
(CHR)
treatment.
This
method
restores
transcriptional
programs,
accessibility,
modification
profiles,
potential,
effectively
recapitulating
competent
anterior
state.
Furthermore,
propose
that
high
bivalency
state
is
defining
feature
human
epiblast.
suggest
marks
drive
regionalization,
ultimately
shaping
cellular
responses
cues.
Язык: Английский