Epigenetic restoration of differentiation competency via reversal of epiblast regionalisation DOI Creative Commons
Magdalena Sutcliffe, Steven W. Wingett, Christopher J. Morris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Abstract Although the epiblast in embryo has capacity to generate all tissues of body, its vitro counterparts often exhibit differentiation biases, posing significant challenges for both basic research and translational applications involving pluripotent stem cells (PSCs). The origins these biases remain incompletely understood. In this study, we identify PSC as arising from fluctuations repressive activating histone posttranslational modifications, leading acquisition a caudal epiblast-like phenotype. We present novel approach overcome bias using chemical chromatin restoration (CHR) treatment. This method restores transcriptional programs, accessibility, modification profiles, potential, effectively recapitulating competent anterior state. Furthermore, propose that high bivalency state is defining feature human epiblast. suggest marks drive regionalization, ultimately shaping cellular responses cues.

Language: Английский

Extra-embryonic mesoderm during development and in in vitro models DOI
Eliana Nehme, Amitesh Panda, Isabelle Migeotte

et al.

Development, Journal Year: 2025, Volume and Issue: 152(5)

Published: March 1, 2025

ABSTRACT Extra-embryonic tissues provide protection and nutrition in vertebrates, as well a connection to the maternal mammals. The extra-embryonic mesoderm is an essential understudied germ layer present amniotes. It involved hematopoiesis, formation of structures such amnion, umbilical cord placenta. origin specification are not entirely conserved across species, molecular mechanisms governing its function fully understood. This Review begins with overview embryonic vertebrates from vivo studies. We then compare vitro models that generate mesoderm-like cells. Finally, we discuss how insights studying both embryos systems can aid designing even more advanced stem cell-based embryo models.

Language: Английский

Citations

0
Stem cell-based models of early human development DOI
Lizhong Liu, Jun Wu

Development, Journal Year: 2025, Volume and Issue: 152(8)

Published: April 15, 2025

ABSTRACT Stem cell-based embryo models (SCBEMs) are structures generated from three-dimensional (3D) culture of pluripotent stem cells and their derivatives, utilizing mechanical and/or chemical cues to facilitate lineage differentiation, self-organization morphogenesis. These partially mimic early embryos, which would otherwise be difficult access. SCBEMs have been established in mice, livestock, nonhuman primates humans. Here, we focus on recently developed human models, with an emphasis the peri-implantation stage aspects development these recapitulate.

Language: Английский

Citations

0
Signaling pathway regulators in preimplantation embryos DOI
Najmeh Karami,

Adeleh Taei,

Poopak Eftekhari-Yazdi

et al.

Journal of Molecular Histology, Journal Year: 2024, Volume and Issue: 56(1)

Published: Dec. 27, 2024

Language: Английский

Citations

1
Building a molecular reference map of the human embryo DOI
Rina C. Sakata, Marta N. Shahbazi

Nature Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Language: Английский

Citations

0
Epigenetic restoration of differentiation competency via reversal of epiblast regionalisation DOI Creative Commons
Magdalena Sutcliffe, Steven W. Wingett, Christopher J. Morris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Abstract Although the epiblast in embryo has capacity to generate all tissues of body, its vitro counterparts often exhibit differentiation biases, posing significant challenges for both basic research and translational applications involving pluripotent stem cells (PSCs). The origins these biases remain incompletely understood. In this study, we identify PSC as arising from fluctuations repressive activating histone posttranslational modifications, leading acquisition a caudal epiblast-like phenotype. We present novel approach overcome bias using chemical chromatin restoration (CHR) treatment. This method restores transcriptional programs, accessibility, modification profiles, potential, effectively recapitulating competent anterior state. Furthermore, propose that high bivalency state is defining feature human epiblast. suggest marks drive regionalization, ultimately shaping cellular responses cues.

Language: Английский

Citations

0