Advanced Functional Materials,
Год журнала:
2024,
Номер
34(19)
Опубликована: Март 4, 2024
Abstract
Biological
activities
take
place
in
3D
environments,
where
cells
interact
various
directions
a
defined,
often
microstructured,
space.
A
sub‐millimeter‐sized
stretching
device
is
developed
to
mechanically
stimulate
structurally
restricted,
soft
multicellular
microenvironment
investigate
the
effect
of
defined
cyclic
mechanical
forces
on
system.
It
consists
multi‐material
microstructure
made
Polydimethylsiloxane
(PDMS)
and
gelatine‐based
hydrogel,
which
printed
using
2‐photon
polymerization
(2PP)
method.
The
structures
are
first
characterized
microscopically
study
different
printing
parameters.
Using
2PP,
organotypic
cell
cultures
then
directly
into
hydrogel
create
true
culture
systems.
These
systems
stimulated
with
cantilever
by
indenting
at
positions.
change
morphology
actin
orientation
when
exposed
stretch,
even
within
short
timescales
30
min.
As
proof
concept,
Medaka
retinal
organoid
encapsulated
same
structure
demonstrate
that
preformed
organoids
can
be
this
results
highlight
capability
2PP
for
manufacturing
multifunctional
devices
control
micrometer
resolution
thus
mimic
stresses
as
they
occur
vivo.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Июль 1, 2024
The
applications
of
hydrogels
have
expanded
significantly
due
to
their
versatile,
highly
tunable
properties
and
breakthroughs
in
biomaterial
technologies.
In
this
review,
we
cover
the
major
achievements
potential
therapeutic
applications,
focusing
primarily
on
two
areas:
emerging
cell-based
therapies
promising
non-cell
modalities.
Within
context
cell
therapy,
discuss
capacity
overcome
existing
translational
challenges
faced
by
mainstream
therapy
paradigms,
provide
a
detailed
discussion
advantages
principal
design
considerations
for
boosting
efficacy
as
well
list
specific
examples
different
disease
scenarios.
We
then
explore
drug
delivery,
physical
intervention
therapies,
other
areas
(e.g.,
bioadhesives,
artificial
tissues,
biosensors),
emphasizing
utility
beyond
mere
delivery
vehicles.
Additionally,
complement
our
latest
progress
clinical
application
outline
future
research
directions,
particularly
terms
integration
with
advanced
biomanufacturing
This
review
aims
present
comprehensive
view
critical
insights
into
selection
both
tailored
meet
requirements
diverse
diseases
situations.
Macromolecules,
Год журнала:
2023,
Номер
56(21), С. 8377 - 8392
Опубликована: Окт. 18, 2023
In
the
past
decade,
stimuli-responsive
hydrogels
are
increasingly
studied
as
biomaterials
for
tissue
engineering
and
regenerative
medicine
purposes.
Smart
can
not
only
replicate
physicochemical
properties
of
extracellular
matrix
but
also
mimic
dynamic
processes
that
crucial
regulation
cell
behavior.
Dynamic
changes
be
influenced
by
hydrogel
itself
(isotropic
vs
anisotropic)
or
guided
applying
localized
triggers.
The
resulting
swelling–shrinking,
shape-morphing,
well
patterns
have
been
shown
to
influence
function
in
a
spatiotemporally
controlled
manner.
Furthermore,
use
bioinks
4D
bioprinting
is
very
promising
they
allow
biofabrication
complex
microstructures.
This
perspective
discusses
recent
cutting-edge
advances
current
challenges
field
smart
engineering.
Additionally,
emerging
trends
potential
future
directions
addressed.
Extracellular
biophysical
properties
have
particular
implications
for
a
wide
spectrum
of
cellular
behaviors
and
functions,
including
growth,
motility,
differentiation,
apoptosis,
gene
expression,
cell–matrix
cell–cell
adhesion,
signal
transduction
mechanotransduction.
Cells
not
only
react
to
unambiguously
mechanical
cues
from
the
extracellular
matrix
(ECM),
but
can
occasionally
manipulate
features
in
parallel
with
biological
characteristics,
thus
interfering
downstream
matrix-based
both
physiological
pathological
processes.
Bidirectional
interactions
between
cells
(bio)materials
vitro
alter
cell
phenotype
mechanotransduction,
as
well
ECM
structure,
intentionally
or
unintentionally.
Interactions
mechanics
vivo
are
importance
variety
diseases,
primarily
cancer.
Stiffness
values
normal
cancerous
tissue
range
500
Pa
(soft)
48
kPa
(stiff),
respectively.
Even
shear
flow
increase
0.1–1
dyn/cm2
(normal
tissue)
1–10
(cancerous
tissue).
There
currently
many
new
areas
activity
tumor
research
on
various
length
scales,
which
highlighted
this
review.
Moreover,
complexity
cancer
is
reduced
common
different
tumors
characteristics
identify
main
pathways
interaction.
This
all
contributes
standardization
mechanotransduction
models
approaches,
which,
ultimately,
increases
understanding
complex
Finally,
effects
mechanics–biology
pairing
key
insights
clinical
practice
treatment
and,
consequently,
translation.
Polymers,
Год журнала:
2023,
Номер
15(19), С. 3940 - 3940
Опубликована: Сен. 29, 2023
Vat
photopolymerization
(VP),
including
stereolithography
(SLA),
digital
light
processing
(DLP),
and
volumetric
printing,
employs
UV
or
visible
to
solidify
cell-laden
photoactive
bioresin
contained
within
a
vat
in
point-by-point,
layer-by-layer,
manner.
VP-based
bioprinting
has
garnered
substantial
attention
both
academia
industry
due
its
unprecedented
control
over
printing
resolution
accuracy,
as
well
rapid
speed.
It
holds
tremendous
potential
for
the
fabrication
of
tissue-
organ-like
structures
field
regenerative
medicine.
This
review
summarizes
recent
progress
VP
fields
tissue
engineering
First,
it
introduces
mechanism
photopolymerization,
followed
by
an
explanation
technique
commonly
used
biomaterials.
Furthermore,
application
was
discussed.
Finally,
challenges
facing
are
discussed,
future
trends
projected.
Nano Letters,
Год журнала:
2024,
Номер
24(21), С. 6376 - 6385
Опубликована: Май 14, 2024
The
fibrous
extracellular
matrix
(ECM)
is
vital
for
tissue
regeneration
and
impacts
implanted
device
treatments.
Previous
research
on
biomaterials
shows
varying
cellular
reactions
to
surface
orientation,
often
due
unclear
interactions
between
topography
substrate
elasticity.
Our
study
addresses
this
gap
by
achieving
the
rapid
creation
of
hydrogels
with
diverse
topographies
moduli
through
a
printing
strategy.
Cells
exhibit
heightened
traction
force
nanopatterned
soft
hydrogels,
particularly
randomly
distributed
patterns
compared
regular
hydrogels.
Meanwhile,
stiff
featuring
an
aligned
topography,
optimal
mechanosensing
observed
random
topography.
Mechanistic
investigations
highlight
that
force-sensing
adhesion
are
influenced
interplay
pattern
deformability
focal
subsequently
mediating
stem
cell
differentiation.
findings
importance
combining
modulus
guide
behavior
in
designing
advanced
engineering
biomaterials.
