Shedding light on 3D printing: Printing photo-crosslinkable constructs for tissue engineering

Biomaterials, Год журнала: 2022, Номер 286, С. 121566 - 121566

Опубликована: Май 10, 2022

Язык: Английский

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Tumor microenvironment: recent advances in understanding and its role in modulating cancer therapies

Medical Oncology, Год журнала: 2025, Номер 42(4)

Опубликована: Март 18, 2025

Язык: Английский

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Dysbiosis and extraintestinal cancers

Journal of Experimental & Clinical Cancer Research, Год журнала: 2025, Номер 44(1)

Опубликована: Фев. 7, 2025

Abstract The gut microbiota plays a crucial role in safeguarding host health and driving the progression of intestinal diseases. Despite recent advances remarkable correlation between dysbiosis extraintestinal cancers, underlying mechanisms are yet to be fully elucidated. Pathogenic microbiota, along with their metabolites, can undermine integrity barrier through inflammatory or metabolic pathways, leading increased permeability translocation pathogens. dissemination pathogens circulation may contribute establishment an immune-suppressive environment that promotes carcinogenesis organs either directly indirectly. oncogenic cascade always engages disruption hormonal regulation responses, induction genomic instability mutations, dysregulation adult stem cell proliferation. This review aims comprehensively summarize existing evidence points potential malignant transformation such as liver, breast, lung, pancreas. Additionally, we delve into limitations inherent current methodologies, particularly challenges associated differentiating low loads gut-derived microbiome within tumors from sample contamination symbiotic microorganisms. Although still controversial, understanding contribution translocated metabolites pathological continuum chronic inflammation could offer novel foundation for development targeted therapeutics.

Язык: Английский

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Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models

ACS Applied Materials & Interfaces, Год журнала: 2022, Номер 14(26), С. 29467 - 29482

Опубликована: Июнь 23, 2022

The tumor extracellular matrix (ECM) plays a vital role in progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of ECM. We developed method for decellularizing delipidating porcine tissue (TDM) compatible with hydrogel formation. addition gelatin methacrylamide alginate allows this TDM bioprinted by itself good printability, shape fidelity, cytocompatibility. Furthermore, bioink has been tuned more closely incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate both bioinks forming cell clusters spheroids. Col1 improves printability as well increases BCC proliferation reduces doxorubicin sensitivity due downregulation HSP90. also allow precise three-dimensional printing scaffolds containing BCCs stromal used fabricate artificial tumors. Taken together, we proven these novel are candidates biofabricating models.

Язык: Английский

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Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Advanced Functional Materials, Год журнала: 2022, Номер 32(29)

Опубликована: Май 22, 2022

Abstract The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate human organoids vitro generation maturation. Considering the importance of supporting matrix, herein is showcased most recent advances bioengineering decellularized tissue hydrogels for generating assembloids. Key design blueprints, characterization methodologies, processing toolboxes are comprehensively discussed light current advances. Such enabling approaches provide grounds engineering next‐generation tissue‐specific with close‐to‐native biomolecular signatures user‐tailored biophysical properties may potentiate physiomimetic potential. In forward looking perspective, combination increasingly complex multicellular assemblies bottom‐up cell technologies unravel unprecedented tissue‐like physiological responses further advance exploitation assembloids as disease surrogates or patient‐tailored living therapeutics.

Язык: Английский

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Organoids and organs-on-chips: insights into predicting the efficacy of systemic treatment in colorectal cancer

Cell Death Discovery, Год журнала: 2023, Номер 9(1)

Опубликована: Фев. 22, 2023

Abstract Cancer heterogeneity has posed a great challenge to traditional cancer treatment, with the reappearance of inter and intra patients being especially critical. Based on this, personalized therapy emerged as significant research focus in recent even future years. Cancer-related therapeutic models are developing, including cell lines, patient-derived xenografts, organoids, etc. Organoids three-dimensional vitro past dozen years able reproduce cellular molecular composition original tumor. These advantages demonstrate potential for organoids develop anticancer therapies, preclinical drug screening prediction patient treatment response. The impact microenvironment cannot be underestimated, remodeling also allows interact other technologies, among which organs-on-chips is representative one. This review highlights use complementary reference tools treating colorectal from perspective clinical efficacy predictability. We discuss limitations both techniques how they complement each well.

Язык: Английский

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Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(26)

Опубликована: Июль 9, 2023

Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes surrounding cells to recapitulate key hallmarks. Collagen is a major extracellular matrix protein solid tumors, low viscosity collagen solution has made bioprinted cancer challenging. This work produces embedded, breast organoid using low-concentration I based bioinks. The biocompatible physically crosslinked silk fibroin hydrogel used generate the bath embedded printing. composition bioink optimized with thermoresponsive hyaluronic acid-based polymer both noninvasive epithelial invasive cells, as well cancer-associated fibroblasts. Mouse organoids are mimic in vivo morphology. A vascularized model created similar strategy, significantly enhanced vasculature formation under hypoxia. study shows great potential utilizing collagen-based advancing understanding cell biology facilitating drug discovery research.

Язык: Английский

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Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting

Gels, Год журнала: 2023, Номер 9(3), С. 195 - 195

Опубликована: Март 3, 2023

As an emerging 3D printing technology, bioprinting has shown great potential in tissue engineering and regenerative medicine. Decellularized extracellular matrices (dECM) have recently made significant research strides been used to create unique tissue-specific bioink that can mimic biomimetic microenvironments. Combining dECMs with may provide a new strategy prepare hydrogels for bioinks hold the construct analogs vitro, similar native tissues. Currently, dECM proven be one of fastest growing bioactive materials plays essential role cell-based bioprinting. This review introduces methods preparing identifying characteristic requirements use The most recent advances dECM-derived are then thoroughly reviewed by examining their application different tissues, such as bone, cartilage, muscle, heart, nervous system, other Finally, generated from is discussed.

Язык: Английский

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Additive manufacturing of promising heterostructure for biomedical applications

International Journal of Extreme Manufacturing, Год журнала: 2023, Номер 5(3), С. 032012 - 032012

Опубликована: Июнь 15, 2023

Abstract As a new generation of materials/structures, heterostructure is characterized by heterogeneous zones with dramatically different mechanical, physical or chemical properties. This endows unique interfaces, robust architectures, and synergistic effects, making it promising option as advanced biomaterials for the highly variable anatomy complex functionalities individual patients. However, main challenges developing lie in control crystal/phase evolution distribution/fraction components structures. In recent years, additive manufacturing techniques have attracted increasing attention due to flexibility tailored structures synthetic multimaterials. review focuses on biomedical applications. The structural features functional mechanisms are summarized. typical material systems heterostructure, mainly including metals, polymers, ceramics, their composites, presented. And resulting effects multiple properties also systematically discussed terms biocompatible, biodegradable, antibacterial, biosensitive magnetostrictive Next, this work outlines research progress employed from aspects advantages, processes, properties, highlights prospective utilization fields, particular bioscaffolds, vasculatures, biosensors biodetections. Finally, future directions breakthroughs prospected focus more applications infection prevention drug delivery.

Язык: Английский

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Nerve‐on‐a‐Chip Derived Biomimicking Microfibers for Peripheral Nerve Regeneration

Advanced Science, Год журнала: 2023, Номер 10(20)

Опубликована: Апрель 29, 2023

Fibrous scaffolds have shown their advantages in tissue engineering, such as peripheral nerve regeneration, while most of the existing fiber-shaped are with simple structures, and vitro performance for regeneration lacks systematic analysis. Here, novel nerve-on-a-chip derived biomimicking microfibers presented. The controllable core-shell structures functionalities generated through capillary microfluidic devices. By integrating these into a multitrack-architectured chip, coculturing them cells well gradient bioactive elements, capabilities systematically assessing performances fiber formation hollow at level is constructed. Based on rat sciatic injury model, rapid promotion ability demonstrated optimized function recovery vivo, which implies credibility evaluation regeneration. Thus, it convinced that organ-on-a-chip will undoubtedly open up new chapter evaluating biological vivo engineering.

Язык: Английский

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