Bioactive Materials,
Год журнала:
2024,
Номер
35, С. 382 - 400
Опубликована: Фев. 14, 2024
Three-dimensional
(3D)
bioprinting,
an
effective
technique
for
building
cell-laden
structures
providing
native
extracellular
matrix
environments,
presents
challenges,
including
inadequate
cellular
interactions.
To
address
these
issues,
cell
spheroids
offer
a
promising
solution
improving
their
biological
functions.
Particularly,
minispheroids
with
50–100
μm
diameters
exhibit
enhanced
maturation.
We
propose
one-step
minispheroid-forming
bioprinting
process
incorporating
electrical
stimulation
(E-MS-printing).
By
stimulating
the
cells,
controlled
were
generated
by
manipulating
bioink
viscosity
and
intensity.
validate
its
feasibility,
E-MS-printing
was
applied
to
fabricate
engineered
liver
model
designed
mimic
hepatic
lobule
unit.
employed
print
hepatocyte
region,
followed
central
vein
using
core-shell
nozzle.
The
resulting
constructs
displayed
liver-mimetic
containing
minispheroids,
which
facilitated
improved
maturation,
functional
attributes,
vessel
formation.
Our
results
demonstrate
new
potential
3D
that
can
replicate
tissues.
Journal of Experimental & Clinical Cancer Research,
Год журнала:
2025,
Номер
44(1)
Опубликована: Фев. 7, 2025
Abstract
The
gut
microbiota
plays
a
crucial
role
in
safeguarding
host
health
and
driving
the
progression
of
intestinal
diseases.
Despite
recent
advances
remarkable
correlation
between
dysbiosis
extraintestinal
cancers,
underlying
mechanisms
are
yet
to
be
fully
elucidated.
Pathogenic
microbiota,
along
with
their
metabolites,
can
undermine
integrity
barrier
through
inflammatory
or
metabolic
pathways,
leading
increased
permeability
translocation
pathogens.
dissemination
pathogens
circulation
may
contribute
establishment
an
immune-suppressive
environment
that
promotes
carcinogenesis
organs
either
directly
indirectly.
oncogenic
cascade
always
engages
disruption
hormonal
regulation
responses,
induction
genomic
instability
mutations,
dysregulation
adult
stem
cell
proliferation.
This
review
aims
comprehensively
summarize
existing
evidence
points
potential
malignant
transformation
such
as
liver,
breast,
lung,
pancreas.
Additionally,
we
delve
into
limitations
inherent
current
methodologies,
particularly
challenges
associated
differentiating
low
loads
gut-derived
microbiome
within
tumors
from
sample
contamination
symbiotic
microorganisms.
Although
still
controversial,
understanding
contribution
translocated
metabolites
pathological
continuum
chronic
inflammation
could
offer
novel
foundation
for
development
targeted
therapeutics.
ACS Applied Materials & Interfaces,
Год журнала:
2022,
Номер
14(26), С. 29467 - 29482
Опубликована: Июнь 23, 2022
The
tumor
extracellular
matrix
(ECM)
plays
a
vital
role
in
progression
and
drug
resistance.
Previous
studies
have
shown
that
breast
tissue-derived
matrices
could
be
an
important
biomaterial
to
recreate
the
complexity
of
ECM.
We
developed
method
for
decellularizing
delipidating
porcine
tissue
(TDM)
compatible
with
hydrogel
formation.
addition
gelatin
methacrylamide
alginate
allows
this
TDM
bioprinted
by
itself
good
printability,
shape
fidelity,
cytocompatibility.
Furthermore,
bioink
has
been
tuned
more
closely
incorporating
collagen
type
I
(Col1).
Breast
cancer
cells
(BCCs)
proliferate
both
bioinks
forming
cell
clusters
spheroids.
Col1
improves
printability
as
well
increases
BCC
proliferation
reduces
doxorubicin
sensitivity
due
downregulation
HSP90.
also
allow
precise
three-dimensional
printing
scaffolds
containing
BCCs
stromal
used
fabricate
artificial
tumors.
Taken
together,
we
proven
these
novel
are
candidates
biofabricating
models.
Advanced Functional Materials,
Год журнала:
2022,
Номер
32(29)
Опубликована: Май 22, 2022
Abstract
The
extracellular
matrix
plays
a
critical
role
in
bioinstructing
cellular
self‐assembly
and
spatial
(re)configuration
processes
that
culminate
human
organoids
vitro
generation
maturation.
Considering
the
importance
of
supporting
matrix,
herein
is
showcased
most
recent
advances
bioengineering
decellularized
tissue
hydrogels
for
generating
assembloids.
Key
design
blueprints,
characterization
methodologies,
processing
toolboxes
are
comprehensively
discussed
light
current
advances.
Such
enabling
approaches
provide
grounds
engineering
next‐generation
tissue‐specific
with
close‐to‐native
biomolecular
signatures
user‐tailored
biophysical
properties
may
potentiate
physiomimetic
potential.
In
forward
looking
perspective,
combination
increasingly
complex
multicellular
assemblies
bottom‐up
cell
technologies
unravel
unprecedented
tissue‐like
physiological
responses
further
advance
exploitation
assembloids
as
disease
surrogates
or
patient‐tailored
living
therapeutics.
Cell Death Discovery,
Год журнала:
2023,
Номер
9(1)
Опубликована: Фев. 22, 2023
Abstract
Cancer
heterogeneity
has
posed
a
great
challenge
to
traditional
cancer
treatment,
with
the
reappearance
of
inter
and
intra
patients
being
especially
critical.
Based
on
this,
personalized
therapy
emerged
as
significant
research
focus
in
recent
even
future
years.
Cancer-related
therapeutic
models
are
developing,
including
cell
lines,
patient-derived
xenografts,
organoids,
etc.
Organoids
three-dimensional
vitro
past
dozen
years
able
reproduce
cellular
molecular
composition
original
tumor.
These
advantages
demonstrate
potential
for
organoids
develop
anticancer
therapies,
preclinical
drug
screening
prediction
patient
treatment
response.
The
impact
microenvironment
cannot
be
underestimated,
remodeling
also
allows
interact
other
technologies,
among
which
organs-on-chips
is
representative
one.
This
review
highlights
use
complementary
reference
tools
treating
colorectal
from
perspective
clinical
efficacy
predictability.
We
discuss
limitations
both
techniques
how
they
complement
each
well.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
12(26)
Опубликована: Июль 9, 2023
Bioinks
for
3D
bioprinting
of
tumor
models
should
not
only
meet
printability
requirements
but
also
accurately
maintain
and
support
phenotypes
surrounding
cells
to
recapitulate
key
hallmarks.
Collagen
is
a
major
extracellular
matrix
protein
solid
tumors,
low
viscosity
collagen
solution
has
made
bioprinted
cancer
challenging.
This
work
produces
embedded,
breast
organoid
using
low-concentration
I
based
bioinks.
The
biocompatible
physically
crosslinked
silk
fibroin
hydrogel
used
generate
the
bath
embedded
printing.
composition
bioink
optimized
with
thermoresponsive
hyaluronic
acid-based
polymer
both
noninvasive
epithelial
invasive
cells,
as
well
cancer-associated
fibroblasts.
Mouse
organoids
are
mimic
in
vivo
morphology.
A
vascularized
model
created
similar
strategy,
significantly
enhanced
vasculature
formation
under
hypoxia.
study
shows
great
potential
utilizing
collagen-based
advancing
understanding
cell
biology
facilitating
drug
discovery
research.
As
an
emerging
3D
printing
technology,
bioprinting
has
shown
great
potential
in
tissue
engineering
and
regenerative
medicine.
Decellularized
extracellular
matrices
(dECM)
have
recently
made
significant
research
strides
been
used
to
create
unique
tissue-specific
bioink
that
can
mimic
biomimetic
microenvironments.
Combining
dECMs
with
may
provide
a
new
strategy
prepare
hydrogels
for
bioinks
hold
the
construct
analogs
vitro,
similar
native
tissues.
Currently,
dECM
proven
be
one
of
fastest
growing
bioactive
materials
plays
essential
role
cell-based
bioprinting.
This
review
introduces
methods
preparing
identifying
characteristic
requirements
use
The
most
recent
advances
dECM-derived
are
then
thoroughly
reviewed
by
examining
their
application
different
tissues,
such
as
bone,
cartilage,
muscle,
heart,
nervous
system,
other
Finally,
generated
from
is
discussed.
International Journal of Extreme Manufacturing,
Год журнала:
2023,
Номер
5(3), С. 032012 - 032012
Опубликована: Июнь 15, 2023
Abstract
As
a
new
generation
of
materials/structures,
heterostructure
is
characterized
by
heterogeneous
zones
with
dramatically
different
mechanical,
physical
or
chemical
properties.
This
endows
unique
interfaces,
robust
architectures,
and
synergistic
effects,
making
it
promising
option
as
advanced
biomaterials
for
the
highly
variable
anatomy
complex
functionalities
individual
patients.
However,
main
challenges
developing
lie
in
control
crystal/phase
evolution
distribution/fraction
components
structures.
In
recent
years,
additive
manufacturing
techniques
have
attracted
increasing
attention
due
to
flexibility
tailored
structures
synthetic
multimaterials.
review
focuses
on
biomedical
applications.
The
structural
features
functional
mechanisms
are
summarized.
typical
material
systems
heterostructure,
mainly
including
metals,
polymers,
ceramics,
their
composites,
presented.
And
resulting
effects
multiple
properties
also
systematically
discussed
terms
biocompatible,
biodegradable,
antibacterial,
biosensitive
magnetostrictive
Next,
this
work
outlines
research
progress
employed
from
aspects
advantages,
processes,
properties,
highlights
prospective
utilization
fields,
particular
bioscaffolds,
vasculatures,
biosensors
biodetections.
Finally,
future
directions
breakthroughs
prospected
focus
more
applications
infection
prevention
drug
delivery.
Fibrous
scaffolds
have
shown
their
advantages
in
tissue
engineering,
such
as
peripheral
nerve
regeneration,
while
most
of
the
existing
fiber-shaped
are
with
simple
structures,
and
vitro
performance
for
regeneration
lacks
systematic
analysis.
Here,
novel
nerve-on-a-chip
derived
biomimicking
microfibers
presented.
The
controllable
core-shell
structures
functionalities
generated
through
capillary
microfluidic
devices.
By
integrating
these
into
a
multitrack-architectured
chip,
coculturing
them
cells
well
gradient
bioactive
elements,
capabilities
systematically
assessing
performances
fiber
formation
hollow
at
level
is
constructed.
Based
on
rat
sciatic
injury
model,
rapid
promotion
ability
demonstrated
optimized
function
recovery
vivo,
which
implies
credibility
evaluation
regeneration.
Thus,
it
convinced
that
organ-on-a-chip
will
undoubtedly
open
up
new
chapter
evaluating
biological
vivo
engineering.