ACS Nano,
Год журнала:
2024,
Номер
18(49), С. 33505 - 33519
Опубликована: Дек. 2, 2024
The
development
of
novel
detection
technology
for
meat
species
authenticity
is
imperative.
Here,
we
developed
a
machine
learning-supported,
dual-channel
biosensor-in-microdroplet
platform
named
CC-drop
(CRISPR/Cas12a
digital
single-molecule
microdroplet
biosensor).
This
strategy
allowed
us
to
quickly
identify
and
analyze
animal-derived
components
in
foods.
biosensor
was
enabled
by
CRISPR/Cas12a-based
fluorescence
lighting-up
detection,
the
nucleic
acid
signals
can
be
recognized
Cas12a–crRNA
binary
complex
trigger
trans-cleavage
any
by-stander
reporter
single-stranded
(ss)
DNA,
which
converted
amplified
fluorescent
readouts.
ultralocalized
reactor
constructed
reducing
reaction
volume
from
up
picoliter
accommodate
aforementioned
further
enhance
sensitivity
even
render
an
amplification-free
detection.
Moreover,
established
smartphone
App
coupled
with
random
forest
learning
model
based
on
parameters
such
as
area,
intensity,
counting
number
ensure
accuracy
image
recording
processing.
sample-to-result
time
within
80
min.
Importantly,
proposed
able
accurately
detect
ND1
(pork-specific)
IL-2
(duck-specific)
genes
deep
processed
meat-derived
foods
that
usually
had
truncated
results
were
more
robust
practical
than
conventional
real-time
polymerase
chain
after
side-by-side
comparison.
All
all,
expected
used
rapid
food
other
detections
future.
Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(37), С. 13577 - 13594
Опубликована: Сен. 1, 2023
Non-nucleic
acid
targets
have
posed
a
serious
challenge
to
food
safety.
The
detection
of
non-nucleic
can
enable
us
monitor
contamination
in
timely
manner.
In
recent
years,
the
CRISPR/Cas
system
has
been
extensively
explored
biosensing.
However,
there
is
lack
summary
CRISPR/Cas-powered
tailored
involved
This
review
comprehensively
summarizes
advances
on
construction
and
promising
applications
field
safety
related
targets.
current
challenges
futuristic
perspectives
are
also
proposed
accordingly.
rapidly
evolving
provided
powerful
propellant
for
target
via
integration
with
aptamer
and/or
DNAzyme.
Compared
traditional
analytical
methods,
conceptually
novel,
essentially
eliminates
dependence
large
instruments,
demonstrates
capability
rapid,
accurate,
sensitive,
on-site
testing.
Sustainability,
Год журнала:
2023,
Номер
15(10), С. 7855 - 7855
Опубликована: Май 11, 2023
Supply
chain
management
can
significantly
benefit
from
contemporary
technologies.
Among
these
technologies,
blockchain
is
considered
suitable
for
anti-counterfeiting
and
traceability
applications
due
to
its
openness,
decentralization,
anonymity,
other
characteristics.
This
article
introduces
different
types
of
blockchains
standard
algorithms
used
in
technology
discusses
their
advantages
disadvantages.
To
improve
the
work
efficiency
systems
supply
chains
reduce
energy
consumption,
this
paper
proposes
a
model
based
on
practical
Byzantine
fault
tolerance
(PBFT)
algorithm
alliance
chains.
uses
credit
evaluation
system
select
primary
node
integrates
weightage
contributors
(WtC)
consensus
mechanism.
decline
success
rate
while
increasing
number
malicious
transaction
nodes,
thereby
reducing
computing
cost.
Additionally,
throughput
algorithmic
increases
rapidly,
reaching
approximately
680
transactions
per
second
(TPS)
about
120
min
after
nodes
are
eliminated.
The
rapidly
as
blacklist
mechanism
reduces
which
improves
system’s
tolerance.
validate
effectiveness
proposed
model,
case
study
was
conducted
using
data
real-life
food
company.
analysis
results
show
that
period
stable
operation
WtCPBFT
overall
communication
cost
reduced,
stability
were
improved,
fault-tolerant
performance
improved.
In
conclusion,
presents
novel
utilizes
PBFT
WtC
security
indicate
effectively
costs,
stability,
enhance
system.
Quality Assurance and Safety of Crops & Foods,
Год журнала:
2023,
Номер
15(3)
Опубликована: Авг. 21, 2023
Urinary
tract
infection
(UTI)
is
responsible
for
a
significant
portion
of
the
overall
expenses
health
care
as
well
mortality
rates
globally.
We
searched
through
available
research
to
evaluate
whether
micronutrient
supplements
affect
frequency
and
intensity
UTI
in
children
Papers
that
investigated
effects
vitamins
D
C,
zinc,
multiple
supplementations
(MMS)
on
incidence
severity
pediatric
patients
were
pooled
together.
A
random
model
was
used
conduct
meta-analysis
data.
Vitamin
supplementation
reduced
risk
shortened
duration
symptoms
children.
If
clinical
diagnosis
or
laboratory
investigation
confirm
UTI,
relative
probability
with
vitamin
remote
from
null
than
using
self-report.
C
administrated,
reduced,
period
manifestations
cut
half.
It
not
possible
determine
impact
zinc
supplements.
According
findings
current
review,
taking
such
D,
could
assist
preventing
lessen
its
outcome
patients.
More
required
establish
effect
individual
combination
micronutrients
treatment
outcomes
UTI.
