Heliyon,
Год журнала:
2024,
Номер
10(11), С. e31788 - e31788
Опубликована: Май 22, 2024
The
fascial
space
of
the
oral
and
maxillofacial
region
contains
loose
connective
tissues,
which
possess
weak
anti-infection
ability
are
often
prone
to
infection,
leading
acute
suppurative
inflammation
sepsis
through
blood.
Although
antibiotic
use
can
reduce
probability
bacterial
infections,
owing
emergence
antibiotic-resistant
bacteria,
search
for
new
antimicrobial
drugs
is
imminent.
Herein,
we
report
a
metal-organic
framework
(MOF)
antibacterial
material
designed
synthesized
with
gallium
(Ga)
as
central
atom,
possesses
significant
antibacterial,
anti-inflammatory,
antioxidant
effects.
Our
data
suggested
that
GA-based
MOFs
(Ga-MOFs;
1
μg/mL)
could
sufficiently
kill
ACS Omega,
Год журнала:
2023,
Номер
8(24), С. 21391 - 21409
Опубликована: Июнь 7, 2023
Biofilm-associated
infections
have
emerged
as
a
significant
public
health
challenge
due
to
their
persistent
nature
and
increased
resistance
conventional
treatment
methods.
The
indiscriminate
usage
of
antibiotics
has
made
us
susceptible
range
multidrug-resistant
pathogens.
These
pathogens
show
reduced
susceptibility
intracellular
survival.
However,
current
methods
for
treating
biofilms,
such
smart
materials
targeted
drug
delivery
systems,
not
been
found
effective
in
preventing
biofilm
formation.
To
address
this
challenge,
nanotechnology
provided
innovative
solutions
formation
by
clinically
relevant
Recent
advances
nanotechnological
strategies,
including
metallic
nanoparticles,
functionalized
dendrimers,
polymeric
cyclodextrin-based
delivery,
solid
lipid
polymer
conjugates,
liposomes,
may
provide
valuable
technological
against
infectious
diseases.
Therefore,
it
is
imperative
conduct
comprehensive
review
summarize
the
recent
advancements
limitations
advanced
nanotechnologies.
present
Review
encompasses
summary
agents,
mechanisms
that
lead
formation,
impact
on
human
health.
In
nutshell,
offers
survey
managing
infections.
A
detailed
presentation
how
these
strategies
improve
control
prevent
key
objective
mechanisms,
applications,
prospects
nanotechnologies
better
understanding
Antimicrobial Resistance and Infection Control,
Год журнала:
2023,
Номер
12(1)
Опубликована: Сен. 7, 2023
Biofilms
are
ubiquitous
in
healthcare
settings.
By
nature,
biofilms
less
susceptible
to
antimicrobials
and
associated
with
healthcare-associated
infections
(HAI).
Resistance
of
biofilm
is
multifactorial
the
presence
a
matrix
composed
extracellular
polymeric
substances
eDNA,
being
major
contributing
factor.
The
usual
multispecies
composition
environmental
can
also
impact
on
antimicrobial
efficacy.
In
settings,
two
main
types
present:
hydrated
biofilms,
for
example,
drains
parts
some
medical
devices
equipment,
dry
(DSB)
surfaces
possibly
devices.
act
as
reservoir
pathogens
including
multi-drug
resistant
organisms
their
elimination
requires
different
approaches.
control
(drain)
should
be
informed
by
reduction
or
microbial
bioburden
together
measuring
regrowth
time.
DSB
measured
combination
decrease
bacterial
transfer
post-intervention.
Failure
increases
risk
HAI,
but
not
solely
responsible
disinfection
failure
shortcoming.
limited
number
standardised
efficacy
tests
hindrance
end
users
manufacturers,
whilst
Europe
there
no
approved
standard
protocols.
Education
stakeholders
about
ad
hoc
tests,
often
academic
thus
paramount,
achieve
better
Abstract
Biofilm‐associated
infections
are
one
of
the
most
challenging
healthcare
threats
for
humans,
accounting
80%
bacterial
infections,
leading
to
persistent
and
chronic
infections.
The
conventional
antibiotics
still
face
their
dilemma
poor
therapeutic
effects
due
high
tolerance
resistance
led
by
biofilm
barriers.
Nanotechnology‐based
antimicrobials,
nanoparticles
(NPs),
paid
attention
extensively
considered
as
promising
alternative.
This
review
focuses
on
whole
journey
NPs
against
biofilm‐associated
clarify
it
clearly,
is
divided
into
four
processes
in
sequence
1)
Targeting
biofilms,
2)
Penetrating
barrier,
3)
Attaching
cells,
4)
Translocating
through
cell
envelope.
Through
outlining
compositions
properties
biofilms
bacteria
recent
advances
present
strategies
each
process
comprehensively
discussed
combat
well
combined
these
with
drug
resistance,
aiming
guide
rational
design
facilitate
wide
application
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Март 9, 2024
The
continuing
emergence
of
new
strains
antibiotic-resistant
bacteria
has
renewed
interest
in
phage
therapy;
however,
there
been
limited
progress
applying
therapy
to
multi-drug
resistant
Mycobacterium
tuberculosis
(Mtb)
infections.
In
this
study,
we
show
that
bacteriophage
D29
and
DS6A
can
efficiently
lyse
Mtb
H37Rv
7H10
agar
plates.
However,
only
kills
liquid
culture
Mtb-infected
human
primary
macrophages.
We
further
subsequent
experiments
that,
after
the
humanized
mice
were
infected
with
aerosolized
H37Rv,
then
treated
intravenously,
showed
increased
body
weight
improved
pulmonary
function
relative
control
mice.
Furthermore,
reduces
load
mouse
organs
greater
efficacy
spleen.
These
results
demonstrate
feasibility
developing
as
an
effective
therapeutic
against
infection.
Annals of Clinical Microbiology and Antimicrobials,
Год журнала:
2024,
Номер
23(1)
Опубликована: Фев. 24, 2024
Abstract
Background
Uropathogenic
Escherichia
coli
(UPEC)
is
the
main
etiological
agent
behind
community-acquired
and
hospital-acquired
urinary
tract
infections
(UTIs),
which
are
among
most
prevalent
human
infections.
The
management
of
UPEC
becoming
increasingly
difficult
owing
to
multi-drug
resistance,
biofilm
formation,
possession
an
extensive
virulence
arsenal.
This
study
aims
characterize
isolates
in
Tanta,
Egypt,
with
regard
their
antimicrobial
phylogenetic
profile,
virulence,
as
well
potential
associations
these
factors.
Methods
One
hundred
were
obtained
from
UTI
patients
Egypt.
Antimicrobial
susceptibility
was
assessed
using
Kirby-Bauer
method.
Extended-spectrum
β-lactamases
(ESBLs)
production
screened
double
disk
synergy
test
confirmed
PCR.
Biofilm
formation
evaluated
microtiter-plate
assay
microscopy-based
techniques.
groups
determined.
hemolytic
activity,
motility,
siderophore
production,
serum
resistance
also
evaluated.
clonal
relatedness
ERIC-PCR.
Results
Isolates
displayed
elevated
cephalosporins
(90–43%),
sulfamethoxazole-trimethoprim
(63%),
ciprofloxacin
(53%).
Ninety
percent
multidrug-resistant
(MDR)/
extensively
drug-resistant
(XDR)
67%
produced
ESBLs.
Notably,
there
inverse
correlation
between
31%,
29%,
32%,
8%
strong,
moderate,
weak,
non-biofilm
producers,
respectively.
Beta-hemolysis,
detected
64%,
84%,
65%,
11%
isolates,
Siderophore
correlated
multiple
antibiotics,
while
hemolysis
more
susceptible
associated
stronger
biofilms.
Phylogroups
B2
D
predominated,
lower
biofilms
group
B2.
ERIC-PCR
revealed
considerable
diversity
isolates.
Conclusion
research
highlights
dissemination
evident
suggests
a
cost
on
bacterial
cells;
that
may
rely
enhance
survival.
emphasizes
importance
considering
ability
during
treatment
avoid
therapeutic
failure
and/or
infection
recurrence.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(45)
Опубликована: Июнь 28, 2024
Abstract
Sonodynamic
therapy
(SDT)
is
promising
for
combating
deep‐seated
infectious
diseases
by
generating
substantial
reactive
oxygen
species
(ROS)
through
the
profound
tissue
penetration
capabilities
of
ultrasound.
However,
compact
protective
structures
bacterial
biofilms
present
a
formidable
challenge,
impeding
ROS
efficacy.
Given
that
have
limited
diffusion
range
and
current
sonosensitizers
struggle
to
infiltrate
biofilms,
complete
eradication
pathogenic
bacteria
often
remains
unachieved.
In
this
study,
mesoporous
titanium
dioxide
(TiO
2
)
nanoparticles
are
engineered
asymmetrically
coated
with
thin
layer
Ag
loaded
L‐arginine
(LA)
construct
ultrasound‐propelled
nanomotors.
These
Ag‐TiO
‐LA
Janus
demonstrate
robust
self‐propulsion
upon
ultrasonic
activation,
allowing
deeper
into
biofilm
matrices
enhancing
localized
disruption
improved
SDT
outcomes.
Additionally,
incorporation
not
only
broadens
TiO
’s
absorption
spectrum
but
also
confers
photothermal
NIR
laser
excitation
at
808
nm.
The
nanomotor
amalgamates
sonodynamic
potential
Ag's
properties,
forging
versatile
antimicrobial
agent
capable
efficient
synergistic
antibacterial
effect
when
subjected
dual
ultrasound
stimuli.
This
innovative,
singularly‐structured
nanoparticle
stands
out
as
an
effective
combatant
against
accelerates
healing
process
infected
wounds,
showcasing
multifaceted
clinical
applications.
British Journal of Biomedical Science,
Год журнала:
2024,
Номер
81
Опубликована: Авг. 7, 2024
The
global
issue
of
antimicrobial
resistance
poses
significant
challenges
to
public
health.
World
Health
Organization
(WHO)
has
highlighted
it
as
a
major
health
threat,
causing
an
estimated
700,000
deaths
worldwide.
Understanding
the
multifaceted
nature
antibiotic
is
crucial
for
developing
effective
strategies.
Several
physiological
and
biochemical
mechanisms
are
involved
in
development
resistance.
Bacterial
cells
may
escape
bactericidal
actions
drugs
by
entering
physiologically
dormant
state
known
bacterial
persistence.
Recent
findings
this
field
suggest
that
persistence
can
be
one
main
sources
chronic
infections.
tolerance
developed
persister
could
tolerate
high
levels
antibiotics
give
rise
offspring.
These
offspring
attributed
mechanisms,
especially
This
review
attempts
shed
light
on
persister-induced
current
therapeutic
npj Biofilms and Microbiomes,
Год журнала:
2022,
Номер
8(1)
Опубликована: Окт. 18, 2022
Abstract
Experimental
evolution
experiments
in
which
bacterial
populations
are
repeatedly
exposed
to
an
antimicrobial
treatment,
and
examination
of
the
genotype
phenotype
resulting
evolved
bacteria,
can
help
shed
light
on
mechanisms
behind
reduced
susceptibility.
In
this
review
we
present
overview
why
it
is
important
include
biofilms
experimental
evolution,
approaches
available
study
what
has
taught
us
about
tolerance
resistance
biofilms.
Finally,
emerging
consensus
view
biofilm
susceptibility
supported
by
data
obtained
during
studies.
