A step forwarded on the in vitro and in vivo validation of rifabutin-loaded liposomes for the management of highly virulent MRSA infections DOI Creative Commons
Mariana Coelho, Jacinta O. Pinho, Sandra N. Pinto

и другие.

Journal of Controlled Release, Год журнала: 2025, Номер 380, С. 348 - 361

Опубликована: Фев. 8, 2025

Staphylococcus aureus (S. aureus) infections, especially methicillin resistant (MRSA), constitute an alarming public health issue due to its association with high mortality, morbidity, and hospitalization costs. The increasing antibiotic resistance biofilm-associated infections of MRSA prompted the discovery novel more effective therapeutic strategies. Our team has been working on alternative therapies against S. infections. For this, we have repurposing existing antibacterial drug, rifabutin (RFB), through a nanotechnological platform, liposomes, aiming promote preferential targeting infected sites maximizing potential effect. RFB formulations commercial strain (MRSA ATCC®-33592), either in planktonic or biofilm forms, was assessed. displayed higher effects towards than vancomycin (VCM), gold standard treatment MBIC50 values 103 > 800 μg/ml, respectively. Moreover, antimicrobial effect RFB-loaded liposomes demonstrated be lipid composition-dependent based MIC50 values, which also confirmed by confocal laser scanning microscopy. These studies supported that for positively charged electrostatic interaction at surface occurs without internalization. On other hand, neutral charge internalization within observed. this liposomal formulation stable human plasma, as 83 % still associated 24 h after incubation 37 °C. proof concept assessed systemic murine models infection. Therapeutic survival rates were evaluated animals induced treated free forms compared negative positive controls. lower infection model, 100 achieved all groups under study. However, model only group incorporated attained. In terms bacterial burden, exhibited levels when VCM, even using dose: 20 vs 40 mg/kg body weight, Overall, constitutes strategy being potentiated nanoplatform.

Язык: Английский

Antimicrobial nanoparticles: current landscape and future challenges DOI Creative Commons
Suresh Kumar Mondal, Sourav Chakraborty, Sounik Manna

и другие.

RSC Pharmaceutics, Год журнала: 2024, Номер 1(3), С. 388 - 402

Опубликована: Янв. 1, 2024

Antimicrobial resistance poses a serious threat to global health, necessitating the exploration of innovative solutions.

Язык: Английский

Процитировано

53
Anti-Biofouling Efficacy of Chitosan Nanocomposites in Shrimp Culture Ponds: A Green Nanotechnology Solution DOI

S. Karthick Raja Namasivayam,

R.S. Arvind Bharani,

K. Samrat

и другие.

Journal of environmental chemical engineering, Год журнала: 2025, Номер unknown, С. 115577 - 115577

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2
Liposomal drug delivery strategies to eradicate bacterial biofilms: Challenges, recent advances, and future perspectives DOI Creative Commons
Vijay Kumar Panthi, Kathryn E. Fairfull‐Smith, Nazrul Islam

и другие.

International Journal of Pharmaceutics, Год журнала: 2024, Номер 655, С. 124046 - 124046

Опубликована: Март 29, 2024

Typical antibiotic treatments are often ineffectual against biofilm-related infections since bacteria residing within biofilms have developed various mechanisms to resist antibiotics. To overcome these limitations, antimicrobial-loaded liposomal nanoparticles a promising anti-biofilm strategy as they demonstrated improved delivery and eradication of in biofilms. Antibiotic-loaded revealed remarkably higher antibacterial activities than free drugs experimental settings. Moreover, can be used efficaciously for the combinational antibiotics other antimicrobial compounds/peptide which facilitate, instance, significant breakdown biofilm matrix, increased bacterial elimination from depletion metabolic activity pathogens. Drug-loaded liposomes mitigated recurrent considered tool address challenges associated resistance. Furthermore, it has been that surface charge polyethylene glycol modification notable impact on their activity. Future investigations should tackle persistent hurdles with development safe effective clinical application investigate novel treatments, including CRISPR-Cas gene editing, natural compounds, phages, nano-mediated approaches. Herein, we emphasize significance inhibition biofilms, challenges, recent advances, future perspectives.

Язык: Английский

Процитировано

15
Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance DOI Creative Commons
Ahmad Almatroudi

Frontiers in Bioscience-Landmark, Год журнала: 2024, Номер 29(4), С. 133 - 133

Опубликована: Март 29, 2024

Biofilms, which consist of microorganisms enclosed in an extracellular polymeric material (EPS), hold immense importance the fields environmental research, industry, and medicine. They play a significant role ecosystem dynamics stability, but they also pose issues such as biofouling, corrosion, pollution. Biofilms medical environments are linked to persistent infections elevated healthcare expenses. The EPS matrix plays crucial maintaining structural integrity antibiotic resistance these structures. research primarily investigates facilitating horizontal gene transfer among biofilm communities, with particular emphasis on its impact this process. process is recognized pivotal mechanism emergence resistance, underscoring function biofilms. analysis highlights financial constraints caused by biofilms several industries. Biofilm-associated sector result escalated treatment expenses extended hospitalization periods. In industrial context, have increasing maintenance product contamination, emphasizing need for efficient management solutions. This review presents most recent progress utilization sophisticated imaging tools molecular methodologies. addition conventional techniques, explores tools, DNA RNA sequencing, conjunction proteomics. These approaches essential assessing genetic metabolic mechanisms that regulate development resistance. underscores significance employing interdisciplinary methodology study By incorporating range approaches, analysis, comprehensive understanding may be achieved. approach opens up possibilities developing novel solutions address negative impacts health, environment.

Язык: Английский

Процитировано

14
Bioinspired green synthesis of ZnO nanoparticles by marine-derived Streptomyces plicatus and its multifaceted biomedicinal properties DOI

Jeesha Soni,

Revathi Duraisamy,

Dhanraj Ganapathy

и другие.

Microbial Pathogenesis, Год журнала: 2024, Номер 193, С. 106758 - 106758

Опубликована: Июнь 19, 2024

Язык: Английский

Процитировано

14
Extracellular synthesis of silver nanoparticle using yeast extracts: antibacterial and seed priming applications DOI Open Access
Dae Young Kim, Min Kim, Jung‐Suk Sung

и другие.

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Янв. 19, 2024

Язык: Английский

Процитировано

13
Biofilm Inhibition on Medical Devices and Implants Using Carbon Dots: An Updated Review DOI
Eepsita Priyadarshini, Rohit Kumar,

Kalpana Balakrishnan

и другие.

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(5), С. 2604 - 2619

Опубликована: Апрель 16, 2024

Biofilms are an intricate community of microbes that colonize solid surfaces, communicating via a quorum-sensing mechanism. These microbial aggregates secrete exopolysaccharides facilitating adhesion and conferring resistance to drugs antimicrobial agents. The escalating global concern over biofilm-related infections on medical devices underscores the severe threat human health. Carbon dots (CDs) have emerged as promising substrate combat disrupt biofilm matrices. Their numerous advantages such facile surface functionalization specific properties, position them innovative anti-biofilm Due their minuscule size, CDs can penetrate cells, inhibiting growth cytoplasmic leakage, reactive oxygen species (ROS) generation, genetic material fragmentation. Research has demonstrated efficacy in biofilms formed by key pathogenic bacteria

Язык: Английский

Процитировано

8
Biogenic Zinc Oxide Nanoparticles: An Insight into the Advancements in Antimicrobial Resistance DOI Creative Commons
Avinash Sharma,

K. Akash,

Swati Kumari

и другие.

ECS Journal of Solid State Science and Technology, Год журнала: 2024, Номер 13(4), С. 047002 - 047002

Опубликована: Апрель 1, 2024

Multidrug resistance (MDR) is a significant global challenge requiring strategic solutions to address bacterial infections. Recent advancements in nanotechnology, particularly the synthesis of zinc oxide nanoparticles (ZnO NPs) using natural agents as stabilizers and reducing agents, have shown promising results combating MDR. These possess strong antimicrobial properties against different strains Gram-positive Gram-negative, making them suitable for various industries, including food, pharmaceuticals, coatings, medical devices. ZnO-NPs work by generating reactive oxygen species, releasing ions (Zn2+), disrupting cell membrane, interfering with metabolic processes genetic material, inducing oxidative stress apoptosis. However, more research needed refine techniques, control size morphology, increase antibacterial efficacy. To fully understand their potential, interactions proteins, DNA, walls must also be examined. Investigating synergistic potential biogenic ZnO NPs conventional treatments could enhance therapeutic effectiveness while minimizing risk emergence. Here we provide insight into bio extracts applications well factors affecting process characterization techniques NPs. studies on activity pathogens mechanisms action are discussed. Furthermore, highlighted.

Язык: Английский

Процитировано

7
Combating bacterial biofilms and related drug resistance: Role of phyto-derived adjuvant and nanomaterials DOI

Neelam Amit Kungwani,

Jibanjyoti Panda,

Awdhesh Kumar Mishra

и другие.

Microbial Pathogenesis, Год журнала: 2024, Номер 195, С. 106874 - 106874

Опубликована: Авг. 23, 2024

Язык: Английский

Процитировано

7
Aptamer-based therapy for fighting biofilm-associated infections DOI Creative Commons
Ana Margarida Sousa, Débora Ferreira, L. R. Rodrigues

и другие.

Journal of Controlled Release, Год журнала: 2024, Номер 367, С. 522 - 539

Опубликована: Фев. 3, 2024

Biofilms are key players in the pathogenesis of most chronic infections associated with host tissue or fluids and indwelling medical devices. These hard to be treated due increased biofilms tolerance towards antibiotics comparison planktonic (or free living) cells. Despite advanced understanding their formation physiology, continue a challenge there is no standardized therapeutic approach clinical practice eradicate them. Aptamers offer distinctive properties, including excellent affinity, selectivity, stability, making them valuable tools for purposes. This review explores flexibility designability aptamers as antibiofilm drugs but, importantly, targeting diverse drug delivery systems. It highlights specific examples application species according different modes action inhibition motility adhesion, blocking quorum sensing molecules, dispersal biofilm-cells state. Moreover, it discusses limitations challenges that impaired an success use on biofilm management, well opportunities related modifications can significantly expand applicability field.

Язык: Английский

Процитировано

6