Methylobacterium symbioticum Applied as a Foliar Inoculant Was Little Effective in Enhancing Nitrogen Fixation and Lettuce Dry Matter Yield

Sustainability, Год журнала: 2024, Номер 16(11), С. 4512 - 4512

Опубликована: Май 26, 2024

Nitrogen (N) is a limiting ecological factor for plant growth in most agroecosystems. Biological N fixation, especially from nodulated legumes, has been promoted recent decades as an alternative or complement to industrially synthesized fertilizers. The possibility of utilizing N-fixing organisms the phyllosphere that demonstrate effectiveness across wide range crops particularly exciting. In this study, we examined capacity and impact on lettuce inoculant recently introduced market, which contains microorganism Methylobacterium symbioticum recommended various cultivated species. A pot experiment was conducted using factorial design, included (No Yes) four rates (0 (N0), 25 (N25), 50 (N50), 100 (N100) kg ha−1 N), with replicates, over growing cycles. had significant effect dry matter yield (DMY) only during second mean values cycles ranged 9.9 13.7 g pot−1 12.6 kg−1 pots received did not receive inoculant, respectively. On other hand, plants exhibited robust response applied soil, showing increases both DMY tissue concentration all Mean treatments N0 N100 5.6 8.9 12.5 16.1 pot−1, tissues varied inversely DMY, indicating concentration/dilution effect. difference between treated untreated plants, used estimate fixed N, very low each soils’ rates, assuming average −1.5, −0.9, 2.4, 6.3 N0, N25, N50, N100, This study emphasized amount supplied by its limited DMY. Generally, biological systems microorganisms, achieving high fixation requires level specificity host plant, condition seems have met lettuce. Considering importance subject, imperative further studies be determine more precisely under what conditions proves valuable input farmers effective method reducing mineral fertilization.

Язык: Английский

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Analysis of diversity and function of epiphytic bacterial communities associated with macrophytes using a metagenomic approach

Microbial Ecology, Год журнала: 2024, Номер 87(1)

Опубликована: Янв. 29, 2024

Abstract Epiphytic bacteria constitute a vital component of aquatic ecosystems, pivotal in regulating elemental cycling. Despite their significance, the diversity and functions epiphytic bacterial communities adhering to various submerged macrophytes remain largely unexplored. In this study, we employed metagenomic approach investigate function associated with six macrophytes: Ceratophyllum demersum , Hydrilla verticillata Myriophyllum verticillatum Potamogeton lucens Stuckenia pectinata Najas marina . The results revealed that predominant species for each plant type included Pseudomonas spp., Microbacterium Stenotrophomonas rhizophila Multiple comparisons linear discriminant analysis effect size indicated significant divergence community composition among macrophytes, 0.3–1% uniquely identified. richness S. significantly differed from both C. H. although no differences were observed evenness. Functionally, notable variations relative abundances genes carbon, nitrogen, phosphorus cycling within on macrophyte hosts. Among these communities, exhibited enrichment related 3-hydroxypropionate bicycle nitrogen assimilation, translocation, denitrification. Conversely, M. showcased linked reductive citric acid cycle (Arnon-Buchanan cycle), pentose phosphate (Calvin polyphosphate degradation, organic metabolism. summary, our findings offer valuable insights into leaves, shedding light roles lake ecosystems.

Язык: Английский

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Plant Growth Promotion and Plant Disease Suppression Induced by Bacillus amyloliquefaciens Strain GD4a

Plants, Год журнала: 2024, Номер 13(5), С. 672 - 672

Опубликована: Фев. 28, 2024

Botrytis cinerea, the causative agent of gray mold disease (GMD), invades plants to obtain nutrients and disseminates through airborne conidia in nature. Bacillus amyloliquefaciens strain GD4a, a beneficial bacterium isolated from switchgrass, shows great potential managing GMD plants. However, precise mechanism by which GD4a confers benefits remains elusive. In this study, an A. thaliana-B. cinerea-B. multiple-scale interaction model was used explore how bacteria play essential roles plant growth promotion, pathogen suppression, immunity boosting. Arabidopsis Col-0 wild-type served as testing ground assess GD4a’s efficacy. Additionally, bacterial enzyme activity targeted metabolite tests were conducted validate for enhancing suppressing pathogens diseases. subjected co-incubation with various bacterial, fungal, oomycete evaluate its antagonistic effectiveness vitro. vivo inoculation assays also carried out investigate role regulating host immunity. Bacterial extracellular exudate (BEE) extracted, purified, untargeted metabolomics analysis. Benzocaine (BEN) analysis selected further study function related mechanisms mutant qRT-PCR Finally, comprehensive formulated summarize applying agricultural systems. Our demonstrates efficacy growth, diseases, bolstering Importantly, produces functional that significantly disrupts pathogenicity B. cinerea inhibiting fungal conidium germination hypha formation. our identifies benzocaine novel small molecule triggers basal defense, ISR, SAR responses can effectively promote suppress disease, boost BEE production diverse gene expression.

Язык: Английский

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Science losing its way: examples from the realm of microbial N2-fixation in cereals and other non-legumes

Plant and Soil, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Abstract Background Despite more than 50 years of research, no robust evidence suggests that inoculation cereals and other non-legumes with free-living and/or endophytic bacteria leads to fixation agronomically significant quantities dinitrogen gas (N 2 ) from the atmosphere. A plethora new products claims increase growth yields major crops through stimulating N -fixation by inoculating bacteria. Scope We review literature on in rhizosphere as endophytes non-legume plants. find unequivocal these fix agriculturally amounts atmosphere non-legumes. Research since 1930s has followed repeated, overlapping cycles have concluded plant-growth-promoting hormones were primary reason for crop response microbial inoculants. Conclusion contend regulations are required prevent sale inoculant unsubstantiated spurious claims. Such should require is provided independently verified, that: (i) bacterium can (i.e. it possesses all genes make nitrogenase), (ii) a clear mechanism protect nitrogenase poisoning free oxygen, (iii) present sufficient numbers throughout cycle plant, (iv) enhanced respiration be detected putative -fixing tissues, (v) growing an N-free medium prolific accumulation nitrogen, (vi) one method used confirm quantitatively inputs field.

Язык: Английский

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Biochar as a carrier for plant growth-promoting bacteria in phytoremediation of pesticides

Journal of Hazardous Materials Advances, Год журнала: 2025, Номер unknown, С. 100673 - 100673

Опубликована: Март 1, 2025

Язык: Английский

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Effects of Zinc Thiazole and Oxytetracycline on the Microbial Metabolism, Antibiotic Resistance, and Virulence Factor Genes of Soil, Earthworm Gut, and Phyllosphere

Environmental Science & Technology, Год журнала: 2023, Номер 58(1), С. 160 - 170

Опубликована: Дек. 26, 2023

Pesticides and antibiotics are believed to increase the incidence of antibiotic resistance genes (ARGs) virulence factor (VFGs), constituting a serious threat global health. However, impact this combined pollution on microbiome that related ARGs VFGs soil–plant–animal systems remain unknown. In study, 60-day microcosm experiment was conducted reveal effects zinc thiazole (ZT) oxytetracycline (OTC) microbial communities, resistomes, factors in soil, earthworm gut, phyllosphere samples using metagenomics. ZT exposure perturbed communities nutrient metabolism increased abundance gut. Combined changed profiles by decreasing diversity phyllosphere. Host-tracking analysis identified some genera, such as Citrobacter Aeromonas, frequent hosts Notably, co-occurrence patterns MGEs were observed metagenome-assembled contigs. More importantly, markedly potentially drug-resistant pathogens Acinetobacter soli junii Overall, study expands our current understanding spread under pollutant-induced stress associated health risks.

Язык: Английский

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Nitrogen fertilization modulates rice phyllosphere functional genes and pathogens through fungal communities

The Science of The Total Environment, Год журнала: 2024, Номер 929, С. 172622 - 172622

Опубликована: Апрель 19, 2024

Язык: Английский

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The plant endomicrobiome: Structure and strategies to produce stress resilient future crop

Current Research in Microbial Sciences, Год журнала: 2024, Номер 6, С. 100236 - 100236

Опубликована: Янв. 1, 2024

Plants have a microbiome, diverse community of microorganisms, including bacteria, fungi, and viruses, living inside on their tissues. Versatile endophytic microorganisms inhabited in every plant part without causing disease develop microbiome or endo-microbiome. Plant endo-microbiome are drawn by the nutrient rich micro-environment, turn some microbes mutualistically endorse protect from adverse environmental stresses. interact within well-designed host equilibrium containing xylem, phloem, nutrients, phytohormones, metabolites shift according to nutritional change. regulate respond variations, pathogens, herbivores producing stress regulators, organic acids, secondary metabolites, hormones as well unknown substances signalling molecules. Endomicrobiome efficiently synthesizes multiple bioactive compounds, phytohormones with high competence. The technological innovation next generation genomics biology high-throughput multiomics techniques stepping stones illumination critical communities functional characterization that aid improving physiology, biochemistry immunity interplay for best crop productivity. This review article contains deeper insight endomicrobiome related research work last years, recruitment, niche development, dynamics, removal mechanisms, services health architecture communication, resilient future crop.

Язык: Английский

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Disentangling the variability of symbiotic nitrogen fixation rate and the controlling factors

Global Change Biology, Год журнала: 2024, Номер 30(3)

Опубликована: Март 1, 2024

Abstract Symbiotic nitrogen (N) fixation (SNF), replenishing bioavailable N for terrestrial ecosystems, exerts decisive roles in cycling and gross primary production. Nevertheless, it remains unclear what determines the variability of SNF rate, which retards accurate prediction global earth system models. This study synthesized 1230 isotopic observations to elucidate governing factors underlying rate. The rates varied significantly from 3.69 12.54 g m −2 year −1 across host plant taxa. traits (e.g. biomass characteristics taxa) far outweighed soil properties climatic explaining variations accounting 79.0% total relative importance. Furthermore, annual yield contributed more than half uptake plants, was consistent different ecosystem types. highlights that biotic factors, especially taxa), play overriding determining rate compared with properties. suite parameters lends support improve module models can provide confidence predicting changes ecosystems.

Язык: Английский

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Microbial communities in the phyllosphere and endosphere of Norway spruce under attack by Heterobasidion

Frontiers in Microbiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 8, 2025

Heterobasidion annosum species complex has been regarded as the most destructive disease agent of conifer trees in boreal forests. Tree microbiome can regulate plant-pathogen interactions by influencing both host resistance and pathogen virulence. Such information would help to improve future health forests explore strategies enhance ecosystem stability. In this study, using next-generation sequencing technology, we investigated microbial community different tree regions (needles, upper stem, lower stem) Norway spruce with without wood decay symptoms. The primary purpose was uncover signature characteristic harbored asymptomatic compared diseased trees. Additionally, study inter-kingdom intra-kingdom (bacteria fungi) symptomatic versus results showed that richness (Chao1) fungi bacteria were higher than (P < 0.05). Compared trees, a abundance Actinobacteriota, bacterial genera Methylocella, Conexibacter, Jatrophihabitans, fungal Mollisia. Fungal communities from same anatomic region differed between Bacterial two stem also distinct possessed less stable network more positive correlations at level, distribution correlation numbers even network. conclusion, attack decreased shifted structure functional varying degrees. enlarged became unstable level due infection.

Язык: Английский

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