Foods,
Год журнала:
2024,
Номер
13(21), С. 3416 - 3416
Опубликована: Окт. 26, 2024
Foodborne
illnesses
pose
a
serious
threat
to
public
health,
with
increasing
global
incidence
rates
driven
by
factors
such
as
rising
meat
consumption.
Rapid
detection
of
foodborne
pathogens
in
is
critical
for
preventing
outbreaks.
This
study
investigates
the
potential
gas
chromatography-mass
spectrometry
(GC-MS)
and
Fourier-transform
infrared
spectroscopy
(FTIR)
identifying
biomarkers
spectral
fingerprints
indicative
raw
chicken
meat.
Raw
broiler
samples
were
surface-sterilized
inoculated
pathogens.
The
challenge
specific
pathogen
physical
quality
parameters
like
pH,
color,
texture,
drip
loss,
water
activity
assessed.
GC-MS
analysis
identified
113
metabolites,
including
ureidopropionic
acid,
5-sulfosalicylic
11,14-eicosadienoic
methyl
ester
E.
coli
O157:H7;
11-bromoundecanoic
neocurdione,
glafenin,
eicosanoic
acid
Salmonella;
azepan-1-yl-acetic
ester,
tramadol,
cytarabine,
dipipanone
Staphylococcus
cyclopentaneundecanoic
phosphonofluoridic
î-n-formyl-l-lysine
Pseudomonas.
Pathway
revealed
involvement
fatty
metabolism
amino
degradation
pathways.
FTIR
data
showed
significant
variances
between
control
spiked
samples,
particularly
region.
metabolites
patterns
could
serve
developing
rapid
methods,
contributing
enhanced
food
safety
protocols.
Centrifugal
microfluidics,
with
its
advantages
of
rapid
and
precise
fluid
control
without
the
need
for
external
pressure,
is
widely
applied
in
point-of-care
testing.
Analytica Chimica Acta,
Год журнала:
2024,
Номер
1305, С. 342507 - 342507
Опубликована: Март 23, 2024
Microfluidic
and
capillary
devices
are
increasingly
being
used
in
analytical
applications
while
their
overall
size
keeps
decreasing.
Detection
sensitivity
for
these
microdevices
gains
more
importance
as
device
sizes
consequently,
sample
volumes,
decrease.
This
paper
reviews
optical,
electrochemical,
electrical,
mass
spectrometric
detection
methods
that
applicable
to
scale
microfluidic
devices,
with
brief
introduction
the
principles
each
case.
Much
of
this
is
considered
context
separations.
We
do
consider
theoretical
aspects
separations
by
open
tubular
liquid
chromatography,
arguably
most
potentially
fertile
area
has
been
left
fallow
largely
because
lack
scale-appropriate
methods.
also
examine
basis
zone
electrophoretic
Optical
discussed
include
UV/Vis
absorbance,
fluorescence,
chemiluminescence
refractometry.
Amperometry
essentially
only
electrochemical
method
microsystems.
Suppressed
conductance
especially
contactless
conductivity
(admittance)
wide
use
ionic
analytes.
integrated
various
spectrometers,
including
ESI-MS,
APCI-MS,
MALDI-MS
discussed.
advantages
disadvantages
compare
best
reported
limits
uniform
a
format
available
information
allows.
While
review
pays
attention
recent
developments,
our
primary
focus
on
novelty
ingenuity
approach,
regardless
when
it
was
first
proposed,
long
can
be
relevant
miniature
platforms.
Microorganisms,
Год журнала:
2023,
Номер
11(4), С. 851 - 851
Опубликована: Март 27, 2023
Cyanobacteria
(blue-green
algae)
can
accumulate
to
form
harmful
algal
blooms
(HABs)
on
the
surface
of
freshwater
ecosystems
under
eutrophic
conditions.
Extensive
HAB
events
threaten
local
wildlife,
public
health,
and
utilization
recreational
waters.
For
detection/quantification
cyanobacteria
cyanotoxins,
both
United
States
Environmental
Protection
Agency
(USEPA)
Health
Canada
increasingly
indicate
that
molecular
methods
be
useful.
However,
each
detection
method
has
specific
advantages
limitations
for
monitoring
HABs
in
water
ecosystems.
Rapidly
developing
modern
technologies,
including
satellite
imaging,
biosensors,
machine
learning/artificial
intelligence,
integrated
with
standard/conventional
overcome
associated
traditional
cyanobacterial
methodology.
We
examine
advances
cell
lysis
methodology
conventional/modern
methods,
imaging
techniques,
polymerase
chain
reaction
(PCR)/DNA
sequencing,
enzyme-linked
immunosorbent
assays
(ELISA),
mass
spectrometry,
remote
sensing,
learning/AI-based
prediction
models.
This
review
focuses
specifically
methodologies
likely
employed
ecosystems,
especially
Great
Lakes
region
North
America.
Graphene
field-effect
transistors
(GFETs)
are
widely
used
in
biosensing
due
to
their
excellent
properties
biomolecular
signal
amplification,
exhibiting
great
potential
for
high-sensitivity
and
point-of-care
testing
clinical
diagnosis.
However,
difficulties
complicated
fabrication
steps
the
main
limitations
further
studies
applications
of
GFETs.
In
this
study,
a
modular
technique
is
introduced
construct
microfluidic
GFET
biosensors
within
3
independent
steps.
The
low-melting
metal
electrodes
intricate
flow
channels
incorporated
maintain
structural
integrity
graphene
facilitate
subsequent
sensing
operations.
as-fabricated
biosensor
demonstrates
long-term
stability,
performs
effectively
various
ion
environments.
It
also
exhibits
high
sensitivity
selectivity
detecting
single-stranded
nucleic
acids
at
10
fm
concentration.
Furthermore,
when
combined
with
CRISPR/Cas12a
system,
it
facilitates
amplification-free
rapid
detection
concentration
1
fm.
Thus,
believed
that
modular-fabricated
may
shed
light
on
development
FET-based
applications.
