BBA Advances, Год журнала: 2025, Номер 7, С. 100151 - 100151
Опубликована: Янв. 1, 2025
Язык: Английский
Advanced Materials, Год журнала: 2024, Номер unknown
Опубликована: Фев. 21, 2024
Miniaturized droplets, characterized by well-controlled microenvironments and capability for parallel processing, have significantly advanced the studies on enzymatic evolution, molecular diagnostics, single-cell analysis. However, manipulation of small-sized including moving, merging, trapping targeted droplets complex biochemical assays subsequent analysis, is not trivial remains technically demanding. Among various techniques, light-driven methods stand out as a promising candidate droplet in facile flexible manner, given features contactless interaction, high spatiotemporal resolution, biocompatibility. This review therefore compiles an in-depth discussion governing mechanisms underpinning manipulation. Besides, light-responsive materials, representing core light-matter interaction key character converting light into different forms energy, are particularly assessed this review. Recent advancements materials most notable applications comprehensively archived evaluated. Continuous innovations rational engineering expected to propel development manipulation, equip with enhanced functionality, broaden routine investigations.
Язык: Английский
Процитировано
17
Chemical Engineering Journal, Год журнала: 2024, Номер 482, С. 148845 - 148845
Опубликована: Янв. 21, 2024
Язык: Английский
Процитировано
15
Nature Food, Год журнала: 2025, Номер unknown
Опубликована: Янв. 2, 2025
Язык: Английский
Процитировано
5
Trends in Food Science & Technology, Год журнала: 2024, Номер 149, С. 104568 - 104568
Опубликована: Май 29, 2024
The increased attention in agri-food quality and safety has led to the progress of DNA-based tools aimed fight adulteration issues. Among various molecular approaches, those ones based on markers DNA barcoding have been adequately validated, whereas new such as droplet digital PCR (ddPCR), isothermal amplification clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) (CRISPR/Cas) system are starting overtake performances former be applied sector. Herein, an overview recent advances exploitations techniques for authenticity traceability fresh processed plant-derived food, feed medicinal products is provided, including research about monitoring contaminant allergen presence. Moreover, potentials flaws these also discussed. Key finding conclusions: technologies represent valuable preventing frauds several vegetable present market spices, extra virgin olive oil, wine, cocoa, plants. application methods can contribute protection consumers all stakeholders involved production distribution.
Язык: Английский
Процитировано
10
Analytical Chemistry, Год журнала: 2024, Номер 96(20), С. 7817 - 7839
Опубликована: Апрель 23, 2024
ADVERTISEMENT RETURN TO ARTICLES ASAPPREVReviewNEXTLab on a Particle TechnologiesRajesh GhoshRajesh GhoshDepartment of Bioengineering, University California, Los Angeles, California 90095, United StatesMore by Rajesh GhoshView Biographyhttps://orcid.org/0000-0002-7408-8944, Alyssa ArnheimAlyssa ArnheimDepartment ArnheimView Biography, Mark van ZeeMark ZeeDepartment ZeeView Lily ShangLily ShangDepartment ShangView Citradewi SoemardyCitradewi SoemardyDepartment SoemardyView Rui-Chian TangRui-Chian TangDepartment TangView Michael MellodyMichael MellodyDepartment MellodyView Sevana BaghdasarianSevana BaghdasarianDepartment BaghdasarianView Edwin Sanchez OchoaEdwin OchoaDepartment OchoaView Shun YeShun YeDepartment YeView Siyu ChenSiyu ChenDepartment ChenView Cayden WilliamsonCayden WilliamsonDepartment WilliamsonView Amrith KarunaratneAmrith KarunaratneDepartment KarunaratneView and Dino Di Carlo*Dino CarloDepartment StatesJonsson Comprehensive Cancer Center, StatesDepartment Mechanical Aerospace Engineering, StatesCalifornia NanoSystems Institute, States*[email protected]More CarloView Biographyhttps://orcid.org/0000-0003-3942-4284Cite this: Anal. Chem. 2024, XXXX, XXX, XXX-XXXPublication Date (Web):April 22, 2024Publication History Received21 March 2024Accepted16 April 2024Revised14 2024Published online22 2024https://doi.org/10.1021/acs.analchem.4c01510© 2024 The Authors. Published American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0. License Summary*You are free to share (copy redistribute) this article in any medium or format within the parameters below:Creative Commons (CC): Creative license.Attribution (BY): Credit must be given creator.Non-Commercial (NC): Only non-commercial uses work permitted. No Derivatives (ND): Derivative works may created for purposes, but sharing prohibited. View full license*DisclaimerThis summary highlights only some key features terms actual license. It not license has no legal value. Carefully review before using these materials. Open Access indicated. Learn MoreArticle Views-Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum text downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (4 MB) Get e-AlertscloseSUBJECTS:Assays,Liquids,Microparticles,Nanoparticles,Peptides proteins e-Alerts
Язык: Английский
Процитировано
8
Molecular Biology Reports, Год журнала: 2025, Номер 52(1)
Опубликована: Янв. 17, 2025
Язык: Английский
Процитировано
1
ACS Synthetic Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 29, 2025
Advancements in molecular diagnostics, such as polymerase chain reaction and next-generation sequencing, have revolutionized disease management prognosis. Despite these advancements the field faces challenges due to high operational costs need for sophisticated equipment highly trained personnel besides having several technical limitations. The emergent of CRISPR/Cas sensing technology is showing promise a new paradigm clinical although widespread adoption remains limited. This perspective paper discusses specific cases where can surmount existing diagnostic methods by stressing significant role that play revolutionizing diagnostics. It underscores urgency importance addressing technological regulatory hurdles must be overcome harness this effectively laboratories.
Язык: Английский
Процитировано
1
Journal of Experimental & Clinical Cancer Research, Год журнала: 2025, Номер 44(1)
Опубликована: Фев. 7, 2025
Abstract The gut microbiota plays a crucial role in safeguarding host health and driving the progression of intestinal diseases. Despite recent advances remarkable correlation between dysbiosis extraintestinal cancers, underlying mechanisms are yet to be fully elucidated. Pathogenic microbiota, along with their metabolites, can undermine integrity barrier through inflammatory or metabolic pathways, leading increased permeability translocation pathogens. dissemination pathogens circulation may contribute establishment an immune-suppressive environment that promotes carcinogenesis organs either directly indirectly. oncogenic cascade always engages disruption hormonal regulation responses, induction genomic instability mutations, dysregulation adult stem cell proliferation. This review aims comprehensively summarize existing evidence points potential malignant transformation such as liver, breast, lung, pancreas. Additionally, we delve into limitations inherent current methodologies, particularly challenges associated differentiating low loads gut-derived microbiome within tumors from sample contamination symbiotic microorganisms. Although still controversial, understanding contribution translocated metabolites pathological continuum chronic inflammation could offer novel foundation for development targeted therapeutics.
Язык: Английский
Процитировано
1
Biomedical Engineering Advances, Год журнала: 2023, Номер 6, С. 100092 - 100092
Опубликована: Май 18, 2023
Digital polymerase chain reaction (dPCR) is an emerging technique for the absolute quantification of target nucleic acids. dPCR got attention as a precise tool in preclinical research, particularly when used to detect genetic mutations and result highly measurements. In dPCR, statistic Poisson distribution was followed random molecules different partitions, which essential quantification. Amplified sequences partitions are identified by fluorescence each partition functions separate PCR microreactor. Without need calibration, percentage PCR-positive sufficient estimate concentration sequence. The present revolution digital made possible advancements microfluidics, provided effective partitioning techniques. this paper, contrast underlying ideas quantitative real-time with measurement acids quantity Polymerase (q-PCR). This review study briefly introduced background compared types PCR, qPCR PCR. fundamental concept also explained compares advantages over analyzes applications diagnostic cancer viral species.
Язык: Английский
Процитировано
19
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 176, С. 117736 - 117736
Опубликована: Май 1, 2024
Язык: Английский
Процитировано
7