Cancer
is
one
of
the
leading
causes
death
worldwide.
Early
cancer
detection
critical
because
it
can
significantly
improve
treatment
outcome
thus
saving
lives,
reducing
suffering,
and
lessening
psychological
economic
burdens.
biomarkers
provide
varied
information
about
cancer,
from
early
malignancy
to
decisions
on
subsequent
monitoring.
A
large
variety
molecular,
histologic,
radiographic,
or
physiological
entities
features
are
amongst
common
types
biomarkers.
Sizeable
recent
methodological
progress
insights
have
promoted
significant
development
in
field
Here
we
an
overview
advancement
knowledge
related
biomolecules
cellular
used
for
detection.
We
examine
data
CAS
Content
Collection,
largest
human-curated
collection
published
scientific
information,
as
well
biomarker
datasets
at
Excelra,
analyze
publication
landscape
research.
also
discuss
evolution
key
concepts,
pipelines,
with
a
particular
focus
pancreatic
liver
cancers,
known
remarkably
difficult
detect
early,
particularly
high
morbidity
mortality.
The
objective
paper
broad
evolving
current
biomarkers,
outline
challenges
evaluate
growth
opportunities,
order
further
efforts
solving
problems
that
remain.
merit
article
stems
extensive,
wide-ranging
coverage
most
up-to-date
scientific
allowing
unique,
unmatched
breadth
analysis
in-depth
insights.
ACS Sensors,
Год журнала:
2024,
Номер
9(5), С. 2413 - 2420
Опубликована: Апрель 18, 2024
The
highly
contagious
nature
and
100%
fatality
rate
contribute
to
the
ongoing
expanding
impact
of
African
swine
fever
virus
(ASFV),
causing
significant
economic
losses
worldwide.
Herein,
we
developed
a
cascaded
colorimetric
detection
using
combination
CRISPR/Cas14a
system,
G-quadruplex
DNAzyme,
microfluidic
paper-based
analytical
device.
This
CRISPR/Cas14a-G4
biosensor
could
detect
ASFV
as
low
5
copies/μL
differentiate
wild-type
mutated
DNA
with
2-nt
difference.
Moreover,
this
approach
was
employed
in
porcine
plasma.
A
broad
linear
range
observed,
limit
spiked
plasma
calculated
be
42–85
copies/μL.
Our
results
indicate
that
paper
platform
exhibits
advantages
high
sensitivity,
excellent
specificity,
cost,
making
it
promising
for
clinical
applications
field
disease
suitable
popularization
low-resourced
areas.
ACS Pharmacology & Translational Science,
Год журнала:
2024,
Номер
7(3), С. 586 - 613
Опубликована: Фев. 14, 2024
Cancer
is
one
of
the
leading
causes
death
worldwide.
Early
cancer
detection
critical
because
it
can
significantly
improve
treatment
outcomes,
thus
saving
lives,
reducing
suffering,
and
lessening
psychological
economic
burdens.
biomarkers
provide
varied
information
about
cancer,
from
early
malignancy
to
decisions
on
subsequent
monitoring.
A
large
variety
molecular,
histologic,
radiographic,
or
physiological
entities
features
are
among
common
types
biomarkers.
Sizeable
recent
methodological
progress
insights
have
promoted
significant
developments
in
field
Here
we
an
overview
advances
knowledge
related
biomolecules
cellular
used
for
detection.
We
examine
data
CAS
Content
Collection,
largest
human-curated
collection
published
scientific
information,
as
well
biomarker
datasets
at
Excelra,
analyze
publication
landscape
research.
also
discuss
evolution
key
concepts
development
pipelines,
with
a
particular
focus
pancreatic
liver
cancers,
which
known
be
remarkably
difficult
detect
particularly
high
morbidity
mortality.
The
objective
paper
broad
evolving
current
outline
challenges
evaluate
growth
opportunities,
order
further
efforts
solving
problems
that
remain.
merit
this
review
stems
extensive,
wide-ranging
coverage
most
up-to-date
allowing
unique,
unmatched
breadth
analysis
in-depth
insights.
Biosensors,
Год журнала:
2025,
Номер
15(3), С. 189 - 189
Опубликована: Март 15, 2025
This
review
explores
the
development,
technological
foundations,
and
applications
of
biosensor
technologies
across
various
fields,
such
as
medicine
for
disease
diagnosis
monitoring,
food
industry.
However,
primary
focus
is
on
their
use
in
detecting
contaminants
pathogens,
well
environmental
monitoring
water
quality
assessment.
The
classifies
different
types
biosensors
based
bioreceptor
transducer,
highlighting
how
they
are
specifically
designed
detection
emerging
(ECs)
pathogens
water.
Key
innovations
this
technology
critically
examined,
including
advanced
techniques
systematic
evolution
ligands
by
exponential
enrichment
(SELEX),
molecularly
imprinted
polymers
(MIPs),
self-assembled
monolayers
(SAMs),
which
enable
fabrication
sensors
with
improved
sensitivity
selectivity.
Additionally,
integration
microfluidic
systems
into
analyzed,
demonstrating
significant
enhancements
performance
speed.
Through
these
advancements,
work
emphasizes
fundamental
role
key
tools
safeguarding
public
health
preserving
integrity.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(39), С. 14720 - 14726
Опубликована: Сен. 18, 2023
CRISPR/Cas
systems
are
powerful
tools
for
sensitive
nucleic
acid
molecular
diagnosis
due
to
their
specific
recognition
and
high
trans-cleavage
activity
have
also
allowed
quantification
of
non-nucleic
targets,
relying
on
a
strategy
convert
the
target
detection
analysis
acids.
Here,
we
describe
CRISPR/Cas12a-powered
immunosorbent
assay
small-molecule
by
using
antibody
coated
microplate
recognize
small
molecule-labeled
active
DNA
(acDNA)
trigger
CRISPR/Cas12a.
In
absence
acDNA
probes
captured
then
activate
Cas12a
in
catalytic
fluorescent
reporters,
generating
strong
fluorescence.
The
presence
targets
displaces
from
antibody,
causing
decrease
reduction
activated
Cas12a,
so
fluorescence
signal
decreases,
molecules
can
be
detected
monitoring
change.
After
systematically
optimizing
experimental
conditions
(e.g.,
reaction),
proposed
method
achieved
three
model
molecules,
biotin,
digoxin,
folic
acid,
with
low
limits,
flexible
concentration
range
was
obtained
simply
changing
amount
immobilized
antibodies.
showed
selectivity
good
applicability
complex
media.
integration
CRISPR/Cas12a
system
improves
analytical
performance
immunoassay,
broadening
facilitating
its
applications
rapid,
simple,
molecule
analysis.
