Biomarkers for early cancer detection: A landscape view of recent advancements, spotlighting pancreatic and liver cancers DOI Creative Commons

Rumiana Tenchov,

Aparna K. Sapra,

Janet M. Sasso

и другие.

Опубликована: Фев. 8, 2024

Cancer is one of the leading causes death worldwide. Early cancer detection critical because it can significantly improve treatment outcome thus saving lives, reducing suffering, and lessening psychological economic burdens. biomarkers provide varied information about cancer, from early malignancy to decisions on subsequent monitoring. A large variety molecular, histologic, radiographic, or physiological entities features are amongst common types biomarkers. Sizeable recent methodological progress insights have promoted significant development in field Here we an overview advancement knowledge related biomolecules cellular used for detection. We examine data CAS Content Collection, largest human-curated collection published scientific information, as well biomarker datasets at Excelra, analyze publication landscape research. also discuss evolution key concepts, pipelines, with a particular focus pancreatic liver cancers, known remarkably difficult detect early, particularly high morbidity mortality. The objective paper broad evolving current biomarkers, outline challenges evaluate growth opportunities, order further efforts solving problems that remain. merit article stems extensive, wide-ranging coverage most up-to-date scientific allowing unique, unmatched breadth analysis in-depth insights.

Язык: Английский

CRISPR/Cas14 and G-Quadruplex DNAzyme-Driven Biosensor for Paper-Based Colorimetric Detection of African Swine Fever Virus DOI

Xue Zhao,

Yawen He,

Shengjie Shao

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(5), С. 2413 - 2420

Опубликована: Апрель 18, 2024

The highly contagious nature and 100% fatality rate contribute to the ongoing expanding impact of African swine fever virus (ASFV), causing significant economic losses worldwide. Herein, we developed a cascaded colorimetric detection using combination CRISPR/Cas14a system, G-quadruplex DNAzyme, microfluidic paper-based analytical device. This CRISPR/Cas14a-G4 biosensor could detect ASFV as low 5 copies/μL differentiate wild-type mutated DNA with 2-nt difference. Moreover, this approach was employed in porcine plasma. A broad linear range observed, limit spiked plasma calculated be 42–85 copies/μL. Our results indicate that paper platform exhibits advantages high sensitivity, excellent specificity, cost, making it promising for clinical applications field disease suitable popularization low-resourced areas.

Язык: Английский

Процитировано

22

Nanomaterials-assisted CRISPR/Cas detection for food safety: Advances, challenges and future prospects DOI

Zhiying Zhao,

Minghui Lu, Nan Wang

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 167, С. 117269 - 117269

Опубликована: Сен. 7, 2023

Язык: Английский

Процитировано

30

CRISPR/Cas-based nanobiosensors: A reinforced approach for specific and sensitive recognition of mycotoxins DOI

Ma Haowei,

Shadi Ebrahimi, Sofiène Mansouri

и другие.

Food Bioscience, Год журнала: 2023, Номер 56, С. 103110 - 103110

Опубликована: Сен. 9, 2023

Язык: Английский

Процитировано

30

Biomarkers for Early Cancer Detection: A Landscape View of Recent Advancements, Spotlighting Pancreatic and Liver Cancers DOI Creative Commons

Rumiana Tenchov,

Aparna K. Sapra,

Janet M. Sasso

и другие.

ACS Pharmacology & Translational Science, Год журнала: 2024, Номер 7(3), С. 586 - 613

Опубликована: Фев. 14, 2024

Cancer is one of the leading causes death worldwide. Early cancer detection critical because it can significantly improve treatment outcomes, thus saving lives, reducing suffering, and lessening psychological economic burdens. biomarkers provide varied information about cancer, from early malignancy to decisions on subsequent monitoring. A large variety molecular, histologic, radiographic, or physiological entities features are among common types biomarkers. Sizeable recent methodological progress insights have promoted significant developments in field Here we an overview advances knowledge related biomolecules cellular used for detection. We examine data CAS Content Collection, largest human-curated collection published scientific information, as well biomarker datasets at Excelra, analyze publication landscape research. also discuss evolution key concepts development pipelines, with a particular focus pancreatic liver cancers, which known be remarkably difficult detect particularly high morbidity mortality. The objective paper broad evolving current outline challenges evaluate growth opportunities, order further efforts solving problems that remain. merit this review stems extensive, wide-ranging coverage most up-to-date allowing unique, unmatched breadth analysis in-depth insights.

Язык: Английский

Процитировано

11

CRISPR integrated biosensors: A new paradigm for cancer detection DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

и другие.

Clinica Chimica Acta, Год журнала: 2025, Номер 569, С. 120179 - 120179

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

2

Comprehensive insights into pathogenicity and emerging CRISPR-integrated biosensors for the early detection of breast cancer biomarkers DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112908 - 112908

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

Biosensor Technologies for Water Quality: Detection of Emerging Contaminants and Pathogens DOI Creative Commons
Antía Fdez-Sanromán, Nuria Bernárdez-Rodas, Emílio Rosales

и другие.

Biosensors, Год журнала: 2025, Номер 15(3), С. 189 - 189

Опубликована: Март 15, 2025

This review explores the development, technological foundations, and applications of biosensor technologies across various fields, such as medicine for disease diagnosis monitoring, food industry. However, primary focus is on their use in detecting contaminants pathogens, well environmental monitoring water quality assessment. The classifies different types biosensors based bioreceptor transducer, highlighting how they are specifically designed detection emerging (ECs) pathogens water. Key innovations this technology critically examined, including advanced techniques systematic evolution ligands by exponential enrichment (SELEX), molecularly imprinted polymers (MIPs), self-assembled monolayers (SAMs), which enable fabrication sensors with improved sensitivity selectivity. Additionally, integration microfluidic systems into analyzed, demonstrating significant enhancements performance speed. Through these advancements, work emphasizes fundamental role key tools safeguarding public health preserving integrity.

Язык: Английский

Процитировано

1

CRISPR/Cas12a regulated dual-channel ratiometric fluorescence biosensing for sensitive and accurate detection of kanamycin DOI
Xia Yi, Min‐Hsien Wu,

Xinyue Yuwen

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113581 - 113581

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1

CRISPR/Cas biosensing technology: From lab assays to integrated portable devices towards wearables DOI

Ruya Shi,

Ling Zhong, Guozhen Liu

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 177, С. 117796 - 117796

Опубликована: Июнь 2, 2024

Язык: Английский

Процитировано

9

CRISPR/Cas12a-Powered Competitive Immunosorbent Assay for Small Molecules DOI

Fengxi Zhu,

Qiang Zhao

Analytical Chemistry, Год журнала: 2023, Номер 95(39), С. 14720 - 14726

Опубликована: Сен. 18, 2023

CRISPR/Cas systems are powerful tools for sensitive nucleic acid molecular diagnosis due to their specific recognition and high trans-cleavage activity have also allowed quantification of non-nucleic targets, relying on a strategy convert the target detection analysis acids. Here, we describe CRISPR/Cas12a-powered immunosorbent assay small-molecule by using antibody coated microplate recognize small molecule-labeled active DNA (acDNA) trigger CRISPR/Cas12a. In absence acDNA probes captured then activate Cas12a in catalytic fluorescent reporters, generating strong fluorescence. The presence targets displaces from antibody, causing decrease reduction activated Cas12a, so fluorescence signal decreases, molecules can be detected monitoring change. After systematically optimizing experimental conditions (e.g., reaction), proposed method achieved three model molecules, biotin, digoxin, folic acid, with low limits, flexible concentration range was obtained simply changing amount immobilized antibodies. showed selectivity good applicability complex media. integration CRISPR/Cas12a system improves analytical performance immunoassay, broadening facilitating its applications rapid, simple, molecule analysis.

Язык: Английский

Процитировано

16