A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications

Translational Oncology, Год журнала: 2024, Номер 50, С. 102121 - 102121

Опубликована: Сен. 14, 2024

Язык: Английский

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Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection

Science and Technology of Advanced Materials, Год журнала: 2024, Номер 25(1)

Опубликована: Апрель 22, 2024

Exosomes, a type of extracellular vesicles, have attracted considerable attention due to their ability provide valuable insights into the pathophysiological microenvironment cells from which they originate. This characteristic implicates potential use as diagnostic disease biomarkers clinically, including cancer, infectious diseases, neurodegenerative disorders, and cardiovascular diseases. Aptasensors, are electrochemical aptamers based biosensing devices, emerged new class powerful detection technology conventional methods like ELISA Western analysis, primarily because capability for high-performance bioanalysis. review covers current research landscape on exosomes utilizing nanoarchitectonics strategy development aptasensors. Strategies involving signal amplification biofouling prevention discussed, with an emphasis nanoarchitectonics-based bio-interfaces, showcasing enhance sensitivity selectivity through optimal conduction mass transport properties. The ongoing challenges broaden clinical applications these biosensors also highlighted.

Язык: Английский

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Microfluidic biosensors for biomarker detection in body fluids: a key approach for early cancer diagnosis

Biomarker Research, Год журнала: 2024, Номер 12(1)

Опубликована: Дек. 5, 2024

Abstract Early detection of cancer significantly improves patient outcomes, with biomarkers offering a promising avenue for earlier and more precise diagnoses. Microfluidic biosensors have emerged as powerful tool detecting these in body fluids, providing enhanced sensitivity, specificity, rapid analysis. This review focuses on recent advances microfluidic from 2018 to 2024, detailing their operational principles, fabrication techniques, integration nanotechnology biomarker detection. Additionally, we reviewed innovations several aspects biosensors, such novel technologies, nanomaterials chip structures, which enhance capabilities. We highlight key pertinent early explore how biosensor technology contribute the evolving landscape personalized medicine. further technologies could be incorporated into clinical diagnostic workflows improve treatment outcomes. These help enable diagnostics. In addition, this addresses important issues enhancing scalability sensitivity settings points out future possibilities combining artificial intelligence diagnostics optimize practical applications. overview aims guide research applications by addressing current challenges identifying opportunities development field research.

Язык: Английский

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CRISPR/Cas13a Trans-Cleavage and Catalytic Hairpin Assembly Cascaded Signal Amplification Powered SERS Aptasensor for Ultrasensitive Detection of Gastric Cancer-Derived Exosomes

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 17, 2024

Cancer-derived exosomes carry a large number of specific molecular profiles from cancer cells and have emerged as ideal biomarkers for early diagnosis. Accurate detection ultralow-abundance in complex biological samples remains great challenge. Herein, novel SERS aptasensor powered by cascaded signal amplification CRISPR/Cas13a

Язык: Английский

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Ultrasensitive Electrochemiluminescence Biosensor Based on DNA-Bio-Bar-Code and Hybridization Chain Reaction Dual Signal Amplification for Exosomes Detection

Analytical Chemistry, Год журнала: 2024, Номер 96(32), С. 13299 - 13307

Опубликована: Авг. 2, 2024

Exosomes have received considerable attention as potent reference markers for the diagnosis of various neoplasms due to their close and direct relationship with proliferation, adhesion, migration tumor. The ultrasensitive detection cancer-derived low-abundance exosomes is imperative, but still a great challenge. Herein, we report an electrochemiluminescence (ECL) biosensor based on DNA-bio-bar-code hybridization chain reaction (HCR)-mediated dual signal amplification exosomes. In this system, two types aptamers were modified magnetic nanoprobe (MNPs) gold nanoparticles (AuNPs) numerous bio-bar-code DNA, respectively, which formed "sandwich" structures in presence specific target separated under field, DNA released by dissolving AuNPs. triggered HCR procedure produce good deal long duplex structure embedding hemin, generated strong ECL coreactors Under optimal conditions, it exhibited linearly ranging from 10

Язык: Английский

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Branched hybridization chain reaction and tetrahedral DNA-based trivalent aptamer powered SERS sensor for ultra-highly sensitive detection of cancer-derived exosomes

Biosensors and Bioelectronics, Год журнала: 2024, Номер 267, С. 116737 - 116737

Опубликована: Сен. 4, 2024

Язык: Английский

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Highly sensitive and aptamer-based electrochemical fipronil biosensor based on primer exchange reaction and catalytic strand displacement dual recycling amplifications

Analytica Chimica Acta, Год журнала: 2025, Номер 1344, С. 343709 - 343709

Опубликована: Янв. 23, 2025

Язык: Английский

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Multivalent Aptamer-Linked Tetrahedron DNA Assisted Catalytic Hairpin Assembly for Accurate Sers Assay of Cancer-Derived Exosomes in Clinical Blood

Опубликована: Янв. 1, 2025

Язык: Английский

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A self-actuated CRISPR/Cas12a feedback amplification platform for ultrasensitive detection of exosome

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113477 - 113477

Опубликована: Март 1, 2025

Язык: Английский

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Multivalent aptamer-linked tetrahedron DNA assisted catalytic hairpin assembly for accurate SERS assay of cancer-derived exosomes in clinical blood

Biosensors and Bioelectronics, Год журнала: 2025, Номер 282, С. 117497 - 117497

Опубликована: Апрель 18, 2025

Язык: Английский

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