Microorganisms,
Год журнала:
2024,
Номер
12(12), С. 2555 - 2555
Опубликована: Дек. 11, 2024
To
ensure
the
safety
of
foodstuffs,
widespread
non-laboratory
monitoring
for
pathogenic
contaminants
is
in
demand.
A
suitable
technique
this
purpose
lateral
flow
immunoassay
(LFIA)
which
combines
simplicity,
rapidity,
and
productivity
with
specific
immune
detection.
This
study
considered
three
developed
formats
LFIA
Salmonella
Typhimurium,
a
priority
contaminant
milk.
Common
sandwich
all
immunoreagents
pre-applied
to
test
strip
(format
A)
was
compared
incubation
sample
(gold
nanoparticle—antibody)
conjugate,
preceding
processes
B),
sequential
passages
conjugate
along
C).
Under
chosen
conditions,
detection
limits
assay
times
were
3
×
104,
1
105,
105
cells/mL,
10,
15,
20
min
A,
B,
C,
respectively.
The
selected
format
successfully
applied
milk
samples.
sample’s
dilution
fat
content
1.0%
causes
pathogen
detection,
70–110%
revealing
1.5–8.5%
accuracy.
obtained
results
demonstrate
that
allows
lower
concentrations
cells
and,
way,
accelerates
decision-making
food
control.
Decentralized
testing
using
multiplex
lateral
flow
assays
(mLFAs)
to
simultaneously
detect
multiple
analytes
can
significantly
enhance
detection
efficiency,
reduce
cost
and
time,
improve
analytic
accuracy.
However,
the
challenges,
including
monochromatic
color
of
probe
particles,
interference
between
different
test
lines,
reduced
specificity
sensitivity,
severely
hinder
mLFAs
from
wide
use.
In
this
study,
we
prepared
polydopamine
(PDA)-coated
dyed
cellulose
nanoparticles
(dCNPs@P)
with
tunable
colors
as
for
mLFAs.
Cellulose
(CNPs)
were
synthesized
uniform
spheric
shapes
sizes.
Dye
molecules
loaded
on
CNPs
via
a
mature
industrial
dyeing
method.
The
PDA
shell
provided
reactive
surface
facile
receptor
conjugation
protected
dye
leaking.
dCNPs@P
displayed
higher
signal
intensity
than
gold
nanoparticles.
They
also
had
stability
tolerate
salt
varied
pH.
dCNP@P-based
successfully
applied
mycotoxins
in
cereals
determine
levels
inflammatory
biomarkers
differentiate
viral
bacterial
infections.
tests
represented
high
accuracy
more
sensitive
quantified
was
accessible
by
measuring
intensities
colorimetric
or
photothermal
signals.
Overall,
study
provides
practical
system
solution
based
colored
dCNPs@P.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(21), С. 8665 - 8673
Опубликована: Май 9, 2024
Prostate-specific
antigen
(PSA)
is
a
key
marker
for
prostate
cancer
diagnosis.
The
low
sensitivity
of
traditional
lateral
flow
immunoassay
(LFIA)
methods
makes
them
unsuitable
point-of-care
testing.
Herein,
we
designed
nanozyme
by
in
situ
growth
Prussian
blue
(PB)
within
the
pores
dendritic
mesoporous
silica
(DMSN).
PB
was
forcibly
dispersed
into
DMSN,
leading
to
an
increase
exposed
active
sites.
Consequently,
atom
utilization
enhanced,
resulting
superior
peroxidase
(POD)-like
activity
compared
that
cubic
PB.
Antibody-modified
DMSN@PB
nanozymes
serve
as
immunological
probes
enzymatic-enhanced
colorimetric
and
photothermal
dual-signal
LFIA
PSA
detection.
After
systematic
optimization,
based
on
successfully
achieves
4-fold
amplification
signal
7
min
through
catalytic
oxidation
chromogenic
substrate
POD-like
activity.
Moreover,
exhibits
excellent
conversion
ability
under
808
nm
laser
irradiation.
Accordingly,
signals
are
introduced
improve
anti-interference
LFIA,
exhibiting
wide
linear
range
(1–40
ng
mL–1)
detection
limit
(0.202
mL–1),
which
satisfies
early
level
cancer.
This
research
provides
more
accurate
reliable
visualization
analysis
methodology
diagnosis
Analytical Chemistry,
Год журнала:
2024,
Номер
96(26), С. 10714 - 10723
Опубликована: Июнь 24, 2024
Excessive
intake
of
estrogen
poses
significant
health
risks
to
the
human
body;
hence,
there
is
a
necessity
develop
rapid
detection
methods
monitor
its
levels
addition.
Gold
nanoparticles
(AuNPs),
commonly
utilized
as
colorimetric
signal
labels,
find
extensive
application
in
lateral
flow
immunoassay
(LFIA).
However,
sensitivity
traditional
AuNPs-LFIA
typically
constrained
by
low
molar
extinction
coefficients
and
reliance
on
single
signal.
Herein,
this
work,
unique
spark-type
AuCuPt
nanoflowers
modified
with
tannic
acid
(AuCuPt@TA)
were
precisely
designed
reasonable
layer-by-layer
element
composition
green
modification.
The
obtained
displays
robust
broadband
absorption
spanning
visible
near-infrared
spectrum,
showcasing
notable
coefficient
2.38
×
10
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 4, 2025
Traditional
lateral
flow
immunochromatography
assays
(LFIAs)
have
faced
low
sensitivity
for
trace
detection
due
to
the
lack
of
colorimetric
brightness.
The
current
strategies
improve
commonly
disadvantages
an
uncontrollable
enhancement
process
or
high
background
interference,
leading
huge
obstacles
signal
readout.
Herein,
in
situ
metal
ion-based
chemical
coordination
amplification
(MICCA)
strategy
has
been
reported.
Metal
ion
clusters
on
metal–organic
frameworks
could
coordinate
with
chromophores
produce
colored
complexes
visual
enhancement.
A
Zr-based
AIEgen
framework
(MAF)
loaded
Prussian
blue
was
chosen
as
dual-mode
tag
and
fluorescent
MAF
be
employed
a
grafting
substrate
deposit
through
Zr4+
arsenazo
III.
MICCA
situ,
controllable,
free
interference.
For
target
cancer
biomarker
alpha-fetoprotein
(AFP),
limit
(LOD)
by
naked
eye
25
ng/mL,
LODs
fluorescence
were
5
which
5-fold
decreased.
Significantly,
MICCA-LFIA
effectively
differentiate
between
AFP-positive
AFP-negative
clinical
serum
samples.
quantitative
results
highly
consistent
(R2
=
0.9927).
This
work
explored
application
reactions
provided
ideas
high-sensitivity
LFIA
development.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 8, 2025
Fluorescence
lateral
flow
immunoassays
(FL-LFIA)
have
attracted
considerable
attention
in
clinical
diagnosis
due
to
their
outstanding
merits
of
affordable,
sensitive,
on-site,
and
quick
detection.
However,
they
are
still
plagued
by
significant
signal
interference,
such
as
autofluorescence
scattered
light.
The
development
high-performance
robust
phosphors,
i.e.,
label
probes
featuring
with
the
character
low/no
optical
background,
remains
a
great
challenge.
Herein,
we
report
novel
visualized
phosphorescence
LFIA
(Phos-LFIA),
where
composite
microspheres,
carbon
dots
(CDs)
covalently
embedded
dendritic
mesoporous
silicon
nanoparticles
(DMSNs),
were
designed
selected
probes.
obtained
CDs@DMSNs
revealed
uniform
morphologies
particle
sizes,
well
ultralong
(lifetime:
1.14
s,
visible
for
over
8
s
naked
eyes)
room
temperature
(RTP)
aqueous
solution.
As
competitive
nanotags,
an
phosphorescence-based
time-gated
cardiac
troponin
I
(cTnI)
without
interference.
fabricated
Phos-LFIA
test
strips
demonstrated
zero-background
applied
highly
sensitive
cTnI
detection
both
buffer
complex
serum
matrix,
corresponding
limits
(LODs)
0.19
0.21
ng/mL,
respectively.
For
validation,
proposed
excellent
analytical
performance
(sensitivity:
95.45%,
specificity:
88.9%,
κ
value:
0.85),
demonstrating
its
potential
rapid
accurate
myocardial
infarction.
This
work
provided
promising
background-free
probe
FL-LFIA,
it
would
also
open
opportunity
developing
screening
platforms
other
targets
through
modifying
different
recognition
ligands
onto
CDs@DMSNs.
Molecules,
Год журнала:
2024,
Номер
29(15), С. 3683 - 3683
Опубликована: Авг. 3, 2024
Interleukin-6
(IL-6)
detection
and
monitoring
are
of
great
significance
for
evaluating
the
progression
many
diseases
their
therapeutic
efficacy.
Lateral
flow
immunoassay
(LFIA)
is
one
most
promising
point-of-care
testing
(POCT)
methods,
yet
suffers
from
low
sensitivity
poor
quantitative
ability,
which
greatly
limits
its
application
in
IL-6
detection.
Hence,
this
work,
we
integrated
Au
Molecules,
Год журнала:
2024,
Номер
29(19), С. 4551 - 4551
Опубликована: Сен. 25, 2024
Immunoassays
have
been
widely
used
for
the
determination
of
various
analytes
in
fields
disease
diagnosis,
food
safety,
and
environmental
monitoring.
Dual-signal
immunoassays
are
now
advanced
integrated
detection
technologies
with
excellent
self-correction
self-validation
capabilities.
In
this
work,
we
summarize
recent
advances
development
optical
electrochemical
dual-signal
immunoassays,
including
colorimetric,
fluorescence,
surface-enhanced
Raman
spectroscopy
(SERS),
electrochemical,
electrochemiluminescence,
photoelectrochemical
methods.
This
review
particularly
emphasizes
working
principle
diverse
utilization
dual-functional
molecules
nanomaterials.
It
also
outlines
challenges
prospects
future
research
on
immunoassays.
