Lateral Flow Immunosensing of Salmonella Typhimurium Cells in Milk: Comparing Three Sequences of Interactions DOI Creative Commons
Nadezhda A. Byzova, Irina V. Safenkova, A. A. Gorbatov

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2555 - 2555

Published: Dec. 11, 2024

To ensure the safety of foodstuffs, widespread non-laboratory monitoring for pathogenic contaminants is in demand. A suitable technique this purpose lateral flow immunoassay (LFIA) which combines simplicity, rapidity, and productivity with specific immune detection. This study considered three developed formats LFIA Salmonella Typhimurium, a priority contaminant milk. Common sandwich all immunoreagents pre-applied to test strip (format A) was compared incubation sample (gold nanoparticle—antibody) conjugate, preceding processes B), sequential passages conjugate along C). Under chosen conditions, detection limits assay times were 3 × 104, 1 105, 105 cells/mL, 10, 15, 20 min A, B, C, respectively. The selected format successfully applied milk samples. sample’s dilution fat content 1.0% causes pathogen detection, 70–110% revealing 1.5–8.5% accuracy. obtained results demonstrate that allows lower concentrations cells and, way, accelerates decision-making food control.

Language: Английский

Colored Cellulose Nanoparticles with High Stability and Easily Modified Surface for Accurate and Sensitive Multiplex Lateral Flow Assay DOI

Yichen Tian,

Lei Chen, Xiaodi Liu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Decentralized testing using multiplex lateral flow assays (mLFAs) to simultaneously detect multiple analytes can significantly enhance detection efficiency, reduce cost and time, improve analytic accuracy. However, the challenges, including monochromatic color of probe particles, interference between different test lines, reduced specificity sensitivity, severely hinder mLFAs from wide use. In this study, we prepared polydopamine (PDA)-coated dyed cellulose nanoparticles (dCNPs@P) with tunable colors as for mLFAs. Cellulose (CNPs) were synthesized uniform spheric shapes sizes. Dye molecules loaded on CNPs via a mature industrial dyeing method. The PDA shell provided reactive surface facile receptor conjugation protected dye leaking. dCNPs@P displayed higher signal intensity than gold nanoparticles. They also had stability tolerate salt varied pH. dCNP@P-based successfully applied mycotoxins in cereals determine levels inflammatory biomarkers differentiate viral bacterial infections. tests represented high accuracy more sensitive quantified was accessible by measuring intensities colorimetric or photothermal signals. Overall, study provides practical system solution based colored dCNPs@P.

Language: Английский

Citations

2
Colorimetric and Photothermal Dual-Modal Switching Lateral Flow Immunoassay Based on a Forced Dispersion Prussian Blue Nanocomposite for the Sensitive Detection of Prostate-Specific Antigen DOI

Haijiang Gong,

Shili Gai,

Yuelin Tao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(21), P. 8665 - 8673

Published: May 9, 2024

Prostate-specific antigen (PSA) is a key marker for prostate cancer diagnosis. The low sensitivity of traditional lateral flow immunoassay (LFIA) methods makes them unsuitable point-of-care testing. Herein, we designed nanozyme by in situ growth Prussian blue (PB) within the pores dendritic mesoporous silica (DMSN). PB was forcibly dispersed into DMSN, leading to an increase exposed active sites. Consequently, atom utilization enhanced, resulting superior peroxidase (POD)-like activity compared that cubic PB. Antibody-modified DMSN@PB nanozymes serve as immunological probes enzymatic-enhanced colorimetric and photothermal dual-signal LFIA PSA detection. After systematic optimization, based on successfully achieves 4-fold amplification signal 7 min through catalytic oxidation chromogenic substrate POD-like activity. Moreover, exhibits excellent conversion ability under 808 nm laser irradiation. Accordingly, signals are introduced improve anti-interference LFIA, exhibiting wide linear range (1–40 ng mL–1) detection limit (0.202 mL–1), which satisfies early level cancer. This research provides more accurate reliable visualization analysis methodology diagnosis

Language: Английский

Citations

13
Enhancing the stability and sensitivity of a lateral flow immunoassay using stabilized AuNPs for the simultaneous detection of sulfonamides and antibacterial synergists in chicken meat DOI
Chen Chen,

Baowei Huang,

Wenqing Xu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 412, P. 135812 - 135812

Published: April 23, 2024

Language: Английский

Citations

10
Layer-By-Layer Designed Spark-Type AuCuPt Alloy with Robust Broadband Absorption to Enhance Sensitivity in Flexible Detection of Estriol by a Lateral Flow Immunoassay DOI

Qiaoying Wu,

Xuechi Yin,

Yuanyuan Cheng

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10714 - 10723

Published: June 24, 2024

Excessive intake of estrogen poses significant health risks to the human body; hence, there is a necessity develop rapid detection methods monitor its levels addition. Gold nanoparticles (AuNPs), commonly utilized as colorimetric signal labels, find extensive application in lateral flow immunoassay (LFIA). However, sensitivity traditional AuNPs-LFIA typically constrained by low molar extinction coefficients and reliance on single signal. Herein, this work, unique spark-type AuCuPt nanoflowers modified with tannic acid (AuCuPt@TA) were precisely designed reasonable layer-by-layer element composition green modification. The obtained displays robust broadband absorption spanning visible near-infrared spectrum, showcasing notable coefficient 2.38 × 10

Language: Английский

Citations

9
Metal Ion-Based Chemical Coordination Amplification: A Chromophore In Situ Deposition Strategy for Visual Sensitivity-Enhanced Lateral Flow Immunochromatography Assays DOI

Jiaren Song,

Changdi Xu,

Shen Zeng

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Traditional lateral flow immunochromatography assays (LFIAs) have faced low sensitivity for trace detection due to the lack of colorimetric brightness. The current strategies improve commonly disadvantages an uncontrollable enhancement process or high background interference, leading huge obstacles signal readout. Herein, in situ metal ion-based chemical coordination amplification (MICCA) strategy has been reported. Metal ion clusters on metal–organic frameworks could coordinate with chromophores produce colored complexes visual enhancement. A Zr-based AIEgen framework (MAF) loaded Prussian blue was chosen as dual-mode tag and fluorescent MAF be employed a grafting substrate deposit through Zr4+ arsenazo III. MICCA situ, controllable, free interference. For target cancer biomarker alpha-fetoprotein (AFP), limit (LOD) by naked eye 25 ng/mL, LODs fluorescence were 5 which 5-fold decreased. Significantly, MICCA-LFIA effectively differentiate between AFP-positive AFP-negative clinical serum samples. quantitative results highly consistent (R2 = 0.9927). This work explored application reactions provided ideas high-sensitivity LFIA development.

Language: Английский

Citations

1
Zero Background Visualizing Phosphorescence Lateral Flow Immunoassay of Cardiac Troponin I for Rapid and Accurate Diagnosis of Myocardial Infarction DOI

Qingwei Song,

Zixuan Liao,

Zheng Lin

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

Fluorescence lateral flow immunoassays (FL-LFIA) have attracted considerable attention in clinical diagnosis due to their outstanding merits of affordable, sensitive, on-site, and quick detection. However, they are still plagued by significant signal interference, such as autofluorescence scattered light. The development high-performance robust phosphors, i.e., label probes featuring with the character low/no optical background, remains a great challenge. Herein, we report novel visualized phosphorescence LFIA (Phos-LFIA), where composite microspheres, carbon dots (CDs) covalently embedded dendritic mesoporous silicon nanoparticles (DMSNs), were designed selected probes. obtained CDs@DMSNs revealed uniform morphologies particle sizes, well ultralong (lifetime: 1.14 s, visible for over 8 s naked eyes) room temperature (RTP) aqueous solution. As competitive nanotags, an phosphorescence-based time-gated cardiac troponin I (cTnI) without interference. fabricated Phos-LFIA test strips demonstrated zero-background applied highly sensitive cTnI detection both buffer complex serum matrix, corresponding limits (LODs) 0.19 0.21 ng/mL, respectively. For validation, proposed excellent analytical performance (sensitivity: 95.45%, specificity: 88.9%, κ value: 0.85), demonstrating its potential rapid accurate myocardial infarction. This work provided promising background-free probe FL-LFIA, it would also open opportunity developing screening platforms other targets through modifying different recognition ligands onto CDs@DMSNs.

Language: Английский

Citations

1
Sensitive detection of ofloxacin by lateral flow immunoassay based on Prussian blue nanoparticles and colloidal gold DOI
Can Li, Shuangshuang Cui, Jian Zhang

et al.

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 131, P. 106262 - 106262

Published: April 20, 2024

Language: Английский

Citations

5
Au Nanoshell-Based Lateral Flow Immunoassay for Colorimetric and Photothermal Dual-Mode Detection of Interleukin-6 DOI Creative Commons
Cong‐Ying Wen,

Yue Dou,

Yao Liu

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3683 - 3683

Published: Aug. 3, 2024

Interleukin-6 (IL-6) detection and monitoring are of great significance for evaluating the progression many diseases their therapeutic efficacy. Lateral flow immunoassay (LFIA) is one most promising point-of-care testing (POCT) methods, yet suffers from low sensitivity poor quantitative ability, which greatly limits its application in IL-6 detection. Hence, this work, we integrated Au

Language: Английский

Citations

5
Overview of the Design and Application of Dual-Signal Immunoassays DOI Creative Commons
Xiaohua Ma,

Yijing Ge,

Ning Xia

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4551 - 4551

Published: Sept. 25, 2024

Immunoassays have been widely used for the determination of various analytes in fields disease diagnosis, food safety, and environmental monitoring. Dual-signal immunoassays are now advanced integrated detection technologies with excellent self-correction self-validation capabilities. In this work, we summarize recent advances development optical electrochemical dual-signal immunoassays, including colorimetric, fluorescence, surface-enhanced Raman spectroscopy (SERS), electrochemical, electrochemiluminescence, photoelectrochemical methods. This review particularly emphasizes working principle diverse utilization dual-functional molecules nanomaterials. It also outlines challenges prospects future research on immunoassays.

Language: Английский

Citations

5
Multifunctional metal-organic frameworks-mediated colorimetric/photothermal immunosensor for highly sensitivity detection of dibutyl phthalate DOI
Wang Chen, Charlie Xiang, Haoran Zhang

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 472, P. 142928 - 142928

Published: Jan. 15, 2025

Language: Английский

Citations

0