Probing Single-Cell Adhesion Kinetics and Nanomechanical Force with Surface Plasmon Resonance Imaging
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
Single
cell
adhesion
plays
a
significant
role
in
numerous
physiological
and
pathological
processes.
Real-time
imaging
quantification
of
single
kinetics
corresponding
cell–substrate
mechanical
interaction
forces
are
crucial
for
elucidating
the
cellular
mechanisms
involved
tissue
formation,
immune
responses,
cancer
metastasis.
Here,
we
present
development
plasmonic-based
nanomechanical
sensing
system
(PNMSi)
real-time
measurement
associated
with
plasmonic
tracking
monitoring
interactions
accompanying
nanoscale
fluctuations.
Both
slow
binding
dynamic
processes
were
tracked
analyzed
thermodynamic
model
to
determine
kinetic
parameters
quantity
forces.
To
demonstrate
capabilities
PNMSi
platform,
examined
across
four
different
surface
modifications,
obvious
alterations
observed,
influenced
by
charges
interfacial
hydrophilicity.
Additionally,
investigated
changes
cells
during
cytoskeleton
modification,
revealing
cross-linking-induced
changes.
Furthermore,
application
capability
system,
profiling
primary
tumor
metastatic
was
explored,
observed
interaction.
The
platform
facilitates
high-throughput
high
sensitivity
serves
as
promising
identifying
biomarkers
metastasis
screening
potential
therapeutic
agents.
Язык: Английский
Mitochondrial signaling pathways and their role in cancer drug resistance
Cellular Signalling,
Год журнала:
2024,
Номер
122, С. 111329 - 111329
Опубликована: Авг. 5, 2024
Язык: Английский
Epithelial–mesenchymal plasticity in cancer: signaling pathways and therapeutic targets
MedComm,
Год журнала:
2024,
Номер
5(8)
Опубликована: Авг. 1, 2024
Abstract
Currently,
cancer
is
still
a
leading
cause
of
human
death
globally.
Tumor
deterioration
comprises
multiple
events
including
metastasis,
therapeutic
resistance
and
immune
evasion,
all
which
are
tightly
related
to
the
phenotypic
plasticity
especially
epithelial–mesenchymal
(EMP).
cells
with
EMP
manifest
in
three
states
as
transition
(EMT),
partial
EMT,
mesenchymal–epithelial
transition,
orchestrate
switch
heterogeneity
tumor
via
transcriptional
regulation
series
signaling
pathways,
transforming
growth
factor‐β,
Wnt/β‐catenin,
Notch.
However,
due
complicated
nature
EMP,
diverse
process
not
fully
understood.
In
this
review,
we
systematically
conclude
biological
background,
regulating
mechanisms
well
role
therapy
response.
We
also
summarize
range
small
molecule
inhibitors,
immune‐related
approaches,
combination
therapies
that
have
been
developed
target
for
outstanding
EMP‐driven
deterioration.
Additionally,
explore
potential
technique
EMP‐based
mechanistic
investigation
research,
may
burst
vigorous
prospects.
Overall,
elucidate
multifaceted
aspects
progression
suggest
promising
direction
treatment
based
on
targeting
EMP.
Язык: Английский
Living in a noisy world—origins of gene expression noise and its impact on cellular decision‐making
FEBS Letters,
Год журнала:
2024,
Номер
598(14), С. 1673 - 1691
Опубликована: Май 9, 2024
The
expression
level
of
a
gene
can
vary
between
genetically
identical
cells
under
the
same
environmental
condition—a
phenomenon
referred
to
as
noise.
Several
studies
have
now
elucidated
central
role
transcription
factors
in
generation
Transcription
factors,
key
components
regulatory
networks,
drive
many
important
cellular
decisions
response
and
signals.
Therefore,
very
relevant
question
is
how
noise
impacts
regulation
influences
decision‐making.
In
this
Review,
we
summarize
current
understanding
molecular
origins
noise,
highlighting
process,
discuss
ways
which
influence
As
advances
single‐cell
technologies
open
new
avenues
for
studying
well
circuits,
better
on
will
implications
biological
processes.
Язык: Английский
Esterase-responsive nanoparticles (ERN): A targeted approach for drug/gene delivery exploits
Ashok Kumar Madikonda,
Amritha Ajayakumar,
Sudeena Nadendla
и другие.
Bioorganic & Medicinal Chemistry,
Год журнала:
2024,
Номер
116, С. 118001 - 118001
Опубликована: Ноя. 14, 2024
Язык: Английский
Texture analysis can predict response to etoposide-doxorubicin-cisplatin in patients with adrenocortical carcinoma
Journal of Endocrinological Investigation,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 9, 2024
Abstract
Background
The
adrenocortical
carcinoma
(ACC)
is
a
rare
and
highly
aggressive
malignancy
originating
from
the
adrenal
cortex.
These
patients
usually
undergo
chemotherapy
with
etoposide,
doxorubicin,
cisplatin
mitotane
(EDP-M)
in
case
of
locally
advanced
or
metastatic
ACC.
Computed
tomography
(CT)
radiomics
showed
to
be
useful
pathologies.
study
aimed
analyze
association
between
response
EDP-M
treatment
CT
textural
features
at
diagnosis
ACCs.
Methods
We
enrolled
17
ACC
who
underwent
before
after
therapy.
was
evaluated
according
RECIST
1.1,
Choi,
volumetric
criteria.
Based
on
aforementioned
criteria,
were
classified
as
responders
not
responders.
Textural
extracted
biggest
lesion
contrast-enhanced
images
LifeX
software.
ROC
curves
drawn
for
variables
that
significantly
different
(
p
<
0.05)
two
groups.
Results
Long-run
high
grey
level
emphasis
(LRHGLE_GLRLM)
histogram
kurtosis
responder
groups
=
0.04)
multivariate
curve
combining
very
good
AUC
(0.900;
95%IC:
0.724-1.000)
discriminating
More
heterogeneous
tissue
texture
initial
staging
could
predict
positive
treatment.
Conclusions
Adrenal
able
therapy
Язык: Английский