The Science of The Total Environment,
Год журнала:
2021,
Номер
799, С. 149401 - 149401
Опубликована: Авг. 2, 2021
Biofilms
represent
an
essential
way
of
life
and
colonization
new
environments
for
microorganisms.
This
feature
is
regulated
by
quorum
sensing
(QS),
a
microbial
communication
system
based
on
autoinducer
molecules,
such
as
N-acyl-homoserine
lactones
(AHLs)
in
Gram
negative
bacteria.
In
artificial
ecosystems,
like
Wastewater
Treatment
Plants
(WWTPs),
biofilm
attachment
filtration
membranes
produces
biofouling.
this
environment,
the
communities
are
mostly
composed
Gram-negative
phyla.
Thus,
we
used
two
AHLs-degrading
enzymes,
obtained
from
Actinoplanes
utahensis
(namely
AuAAC
AuAHLA)
to
determine
effects
degradation
QS
signals
formation,
among
other
virulence
factors,
Pseudomonas
aeruginosa
strain
isolated
WWTP,
assessing
molecular
mechanisms
through
transcriptomics.
Besides,
studied
possible
community
composition
biofilms
activated
sludge
samples.
Although
enzymes
only
degraded
AHLs
involved
one
four
systems
P.
aeruginosa,
these
activities
produced
deregulation
complete
network.
fact,
-the
enzyme
with
higher
catalytic
efficiency-
deregulated
all
systems.
However,
both
reduced
formation
pyocyanin
protease
production.
The
transcriptomic
response
affected
related
genes,
moreover,
mainly
genes.
Regarding
biofilms,
expected,
abundance
phyla
was
significantly
decreased
after
enzymatic
treatment.
These
results
support
potential
use
method
reduce
WWTP
ameliorate
bacterial
virulence.
Molecules,
Год журнала:
2022,
Номер
27(21), С. 7584 - 7584
Опубликована: Ноя. 4, 2022
Microbial
biodiversity
includes
biotic
and
abiotic
components
that
support
all
life
forms
by
adapting
to
environmental
conditions.
Climate
change,
pollution,
human
activity,
natural
calamities
affect
microbial
biodiversity.
Microbes
have
diverse
growth
conditions,
physiology,
metabolism.
Bacteria
use
signaling
systems
such
as
quorum
sensing
(QS)
regulate
cellular
interactions
via
small
chemical
molecules
which
also
help
with
adaptation
under
undesirable
survival
Proteobacteria
acyl-homoserine
lactone
(AHL)
autoinducers
sense
population
density
modulate
gene
expression.
The
LuxI-type
enzymes
synthesize
AHL
molecules,
while
the
LuxR-type
proteins
(AHL
transcriptional
regulators)
bind
AHLs
QS-dependent
Diverse
been
identified,
diversity
extends
synthases
receptors.
This
review
comprehensively
explains
molecular
of
Pseudomonas
aeruginosa,
Chromobacterium
violaceum,
Agrobacterium
tumefaciens,
Escherichia
coli.
regulatory
mechanism
is
highlighted
in
this
review,
adds
current
understanding
Gram-negative
bacteria.
We
summarize
among
well-studied
QS
recent
advances
role
bacterial
pathways.
describes
AHL-dependent
details
bacteria
can
be
employed
understand
their
features,
improve
adaptation,
develop
broad
biomolecule-based
biotechnological
applications.
Environmental Science & Technology,
Год журнала:
2022,
Номер
56(17), С. 12645 - 12655
Опубликована: Июль 26, 2022
N-acyl-homoserine
lactones
(AHLs)
as
autoinducers
of
Gram-negative
bacteria
for
quorum
sensing
regulation
have
shown
positive
effects
on
the
production
aromatic
proteins
in
extracellular
polymeric
substances
(EPSs)
during
bioflocculation.
To
investigate
role
AHLs
protein
production,
a
Chlorella–bacteria
system
with
great
bioflocculation
was
established
via
fed-batch
cultivation.
Tryptophan
and
main
compounds
EPS
bioflocs
showed
an
increasing
trend
The
Chlorella
cells
only
secreted
tryptophan
rather
than
axenic
N-dodecanoyl-l-homoserine
lactone
(C12-HSL)
correlated
flocculation
activity
content
addition
exogenous
C12-HSL
enhanced
EPS.
sensed
significantly
upregulated
synthesis
pathway
In
addition,
vanillin
quorum-sensing
inhibitor
suppressed
effect
synthesis.
This
result
indicated
that
intercepts
response
to
C12-HSL.
Overall,
is
supposed
be
important
signal
molecule
achieve
communication
between
subsequently
induce
produce
biofloc
formation.
Metabolites,
Год журнала:
2023,
Номер
13(1), С. 114 - 114
Опубликована: Янв. 10, 2023
Quorum
sensing
(QS)
is
a
system
of
communication
bacterial
cells
by
means
chemical
signals
called
autoinducers,
which
modulate
the
behavior
entire
populations
Gram-negative
and
Gram-positive
bacteria.
Three
classes
signaling
molecules
have
been
recognized,
Al-1,
Al-2,
Al-3,
whose
functions
are
slightly
different.
However,
phenomenon
quorum
not
only
concerned
with
interactions
between
bacteria,
but
whole
spectrum
interspecies
interactions.
A
growing
number
research
results
confirm
important
role
QS
in
growth
stimulation
defense
responses
plants.
Although
many
details
concerning
metabolites
rhizosphere
microflora
plant
host
still
unknown,
Al-1
compounds
should
be
considered
as
components
bacterial–plant
interactions,
leading
to
biological
control
phytopathogens.
The
use
class
1
autoinducers
plants
induce
beneficial
activity
may
practical
solution
improve
productivity
under
field
conditions.
In
addition,
researchers
also
interested
tools
that
offer
possibility
regulating
degrading
enzymes
or
specific
inhibitors
(QSI).
Current
knowledge
QSI
provides
an
excellent
foundation
for
application
biopreparations
agriculture,
containing
consortia
AHL-producing
bacteria
limiting
phytopathogenic
organisms.
