Journal of Cellular and Molecular Medicine,
Год журнала:
2025,
Номер
29(2)
Опубликована: Янв. 1, 2025
Postmenopausal
osteoporosis
(PMOP)
is
a
chronic
systemic
bone
metabolism
disorder.
Promotion
in
the
patterns
of
human
marrow
mesenchymal
stem
cells
(hBMSCs)
differentiation
towards
osteoblasts
contributes
to
alleviating
osteoporosis.
Aucubin,
natural
compound
isolated
from
well-known
herbal
medicine
Eucommia,
was
previously
shown
possess
various
pharmacological
effects.
However,
its
effects
on
hBMSCs
PMOP
patients
are
unknown.
The
aim
this
present
research
investigate
impact
and
underlying
process
aucubin
cell
proliferation
osteogenic
patients.
ability
inhibit
ferroptosis
induced
by
erastin
detected;
ROS
production,
ferrous
ion
levels,
SOD,
MDA,
GPX
activities
were
tested
using
commercial
kits.
Next,
ALP
staining,
ARS
RT-qPCR,
RNA-sequencing,
Western
blot
applied
for
determining
mRNA
protein
expression
levels
associated
with
osteogenesis
hBMSCs.
study
also
explored
involvement
BMP2/Smads
signalling
promoting
evaluated
intervention
an
ovariectomised
rat
model.
results
indicated
that
significantly
inhibited
generation
oxidative
stress
protected
against
Additionally,
facilitated
activating
BMP2/SMADs
pathway
attenuated
progression
OVX
rats,
suggesting
potential
therapeutic
benefit
postmenopausal
(PMOP).
Cells,
Год журнала:
2023,
Номер
12(10), С. 1416 - 1416
Опубликована: Май 17, 2023
Extracellular
vesicles
(EVs)
such
as
ectosomes
and
exosomes
have
gained
attention
promising
natural
carriers
for
drug
delivery.
Exosomes,
which
range
from
30
to
100
nm
in
diameter,
possess
a
lipid
bilayer
are
secreted
by
various
cells.
Due
their
high
biocompatibility,
stability,
low
immunogenicity,
favored
cargo
carriers.
The
membrane
of
also
offers
protection
against
degradation,
making
them
desirable
candidate
However,
loading
into
remains
be
challenge.
Despite
strategies
incubation,
electroporation,
sonication,
extrusion,
freeze–thaw
cycling,
transfection
that
been
developed
facilitate
loading,
inadequate
efficiency
still
persists.
This
review
an
overview
current
delivery
using
summarizes
recent
approaches
small-molecule,
nucleic
acid,
protein
drugs
exosomes.
With
insights
these
studies,
we
provide
ideas
more
efficient
effective
molecules
Bioactive Materials,
Год журнала:
2023,
Номер
28, С. 273 - 283
Опубликована: Июнь 1, 2023
Diabetes
mellitus
is
a
chronically
inflamed
disease
that
predisposes
to
delayed
fracture
healing.
Macrophages
play
key
role
in
the
process
of
healing
by
undergoing
polarization
into
either
M1
or
M2
subtypes,
which
respectively
exhibit
pro-inflammatory
anti-inflammatory
functions.
Therefore,
modulation
macrophage
subtype
beneficial
for
Exosomes
perform
an
important
improving
osteoimmune
microenvironment
due
their
extremely
low
immunogenicity
and
high
bioactivity.
In
this
study,
we
extracted
M2-exosomes
used
them
intervene
bone
repair
diabetic
fractures.
The
results
showed
significantly
modulate
decreasing
proportion
macrophages,
thereby
accelerating
We
further
confirmed
induced
conversion
macrophages
stimulating
PI3K/AKT
pathway.
Our
study
offers
fresh
perspective
potential
therapeutic
approach
improve
Abstract
Osteoporosis
is
a
widely
observed
condition
characterized
by
the
systemic
deterioration
of
bone
mass
and
microarchitecture,
which
increases
patient
susceptibility
to
fragile
fractures.
The
intricate
mechanisms
governing
homeostasis
are
substantially
impacted
extracellular
vesicles
(EVs),
play
crucial
roles
in
both
pathological
physiological
contexts.
EVs
derived
from
various
sources
exert
distinct
effects
on
osteoporosis.
Specifically,
released
osteoblasts,
endothelial
cells,
myocytes,
mesenchymal
stem
cells
contribute
formation
due
their
unique
cargo
proteins,
miRNAs,
cytokines.
Conversely,
secreted
osteoclasts
immune
promote
resorption
inhibit
formation.
Furthermore,
use
as
therapeutic
modalities
or
biomaterials
for
diagnosing
managing
osteoporosis
promising.
Here,
we
review
current
understanding
impact
homeostasis,
including
classification
biogenesis
regulatory
present
an
overview
latest
research
progress
treating
using
EVs.
Finally,
discuss
challenges
prospects
translational
Journal of Extracellular Vesicles,
Год журнала:
2024,
Номер
13(4)
Опубликована: Апрель 1, 2024
Osteoporosis
(OP)
is
a
systematic
bone
disease
characterized
by
low
mass
and
fragile
microarchitecture.
Conventional
treatment
for
OP
has
limited
efficacy
long-term
toxicity.
Synthetic
biology
makes
bacterial
extracellular
vesicle
(BEVs)-based
therapeutic
strategies
promising
alternative
the
of
OP.
Here,
we
constructed
recombinant
probiotics
Escherichia
coli
Nissle
1917-pET28a-ClyA-BMP-2-CXCR4
(ECN-pClyA-BMP-2-CXCR4),
in
which
BMP-2
CXCR4
were
overexpressed
fusion
with
BEVs
surface
protein
ClyA.
Subsequently,
isolated
engineered
BEVs-BMP-2-CXCR4
(BEVs-BC)
therapy.
The
BEVs-BC
exhibited
great
targeting
vivo.
In
addition,
had
good
biocompatibility
remarkable
ability
to
promote
osteogenic
differentiation
BMSCs.
Finally,
synthetic
biology-based
significantly
prevented
an
ovariectomized
(OVX)
mouse
model.
conclusion,
both
bone-targeting
bone-forming
one-step
using
biology,
provides
effective
strategy
potential
industrialization.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(22)
Опубликована: Май 21, 2024
The
repair
and
regeneration
of
cartilage
has
always
been
a
hot
topic
in
medical
research.
Cartilage
organoids
(CORGs)
are
special
tissue
created
using
engineering
techniques
outside
the
body.
These
engineered
tissues
provide
models
that
simulate
complex
biological
functions
cartilage,
opening
new
possibilities
for
regenerative
medicine
treatment
strategies.
However,
it
is
crucial
to
establish
suitable
matrix
scaffolds
cultivation
CORGs.
In
recent
years,
utilizing
hydrogel
culture
stem
cells
induce
their
differentiation
into
chondrocytes
emerged
as
promising
method
vitro
construction
this
review,
methods
establishing
CORGs
summarized
an
overview
advantages
limitations
matrigel
such
provided.
Furthermore,
importance
ECM
alternative
substitutes
Matrigel,
alginate,
peptides,
silk
fibroin,
DNA
derivatives
discussed,
pros
cons
these
hydrogels
outlined.
Finally,
challenges
future
directions
research
discussed.
It
hoped
article
provides
valuable
references
design
development
Cell Reports Medicine,
Год журнала:
2024,
Номер
5(5), С. 101522 - 101522
Опубликована: Май 1, 2024
Neuroinflammation
plays
a
significant
role
in
ischemic
injury,
which
can
be
promoted
by
oxidized
mitochondrial
DNA
(Ox-mtDNA).
Cytidine/uridine
monophosphate
kinase
2
(CMPK2)
regulates
mtDNA
replication,
but
its
neuroinflammation
and
injury
remains
unknown.
Here,
we
report
that
CMPK2
expression
is
upregulated
monocytes/macrophages
microglia
post-stroke
humans
mice,
respectively.
Microglia/macrophage
knockdown
using
the
Cre
recombination-dependent
adeno-associated
virus
suppresses
inflammatory
responses
brain,
reduces
infarcts,
improves
neurological
outcomes
CX3CR1Cre/ERT2
mice.
Mechanistically,
limits
newly
synthesized
Ox-mtDNA
formation
subsequently
blocks
NLRP3
inflammasome
activation
microglia/macrophages.
Nordihydroguaiaretic
acid
(NDGA),
as
inhibitor,
discovered
to
reduce
mice
prevent
primary
human
monocytes
from
patients.
Thus,
these
findings
identify
promising
therapeutic
target
for
stroke
other
brain
disorders
associated
with
neuroinflammation.
This
review
highlights
recent
progress
in
exosome-based
drug
delivery
for
cancer
therapy,
covering
exosome
biogenesis,
cargo
selection
mechanisms,
and
their
application
across
multiple
types.
As
small
extracellular
vesicles,
exosomes
exhibit
high
biocompatibility
low
immunogenicity,
making
them
ideal
vehicles
capable
of
efficiently
targeting
cells,
minimizing
off-target
damage
side
effects.
aims
to
explore
the
potential
with
a
focus
on
applications
chemotherapy,
gene
immunomodulation.
Additionally,
challenges
related
production
standardization
are
analyzed,
highlighting
importance
addressing
these
issues
clinical
application.
In
conclusion,
systems
offer
promising
future
therapies.
Further
research
should
aim
enhance
efficiency
facilitate
translation,
paving
way
innovative
treatment
strategies.
Bioactive Materials,
Год журнала:
2023,
Номер
25, С. 29 - 41
Опубликована: Янв. 20, 2023
The
necessity
of
disease
models
for
bone/cartilage
related
disorders
is
well-recognized,
but
the
barrier
between
ex-vivo
cell
culture,
animal
and
real
human
body
has
been
pending
decades.
organoid-on-a-chip
technique
showed
opportunity
to
revolutionize
basic
research
drug
screening
diseases
like
osteoporosis
arthritis.
organoid
on-chip
(BCoC)
system
a
novel
platform
multi-tissue
which
faithfully
emulate
essential
elements,
biologic
functions
pathophysiological
response
under
circumstances.
In
this
review,
we
propose
concept
BCoC
platform,
summarize
modules
current
efforts
orchestrate
them
on
single
microfluidic
system.
Current
models,
unsolved
problems
future
challenging
are
also
discussed,
aim
should
be
deeper
understanding
diseases,
ultimate
realization
generic
tools
further
therapeutic
strategies
pathological
conditions.
