Nanomaterials-driven innovative electrochemiluminescence aptasensors in reporting food pollutants

Coordination Chemistry Reviews, Год журнала: 2023, Номер 485, С. 215136 - 215136

Опубликована: Март 27, 2023

Язык: Английский

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Multifunctional Photoelectrochemical Biosensor Based on ZnIn₂S₄/ZnS QDs@Au–Ag-Reversed Photocurrent of Cu-Metal–Organic Framework Coupled with CRISPR/Cas-12a-Shearing for Assay of Dual Targets

Analytical Chemistry, Год журнала: 2023, Номер 95(17), С. 7053 - 7061

Опубликована: Апрель 20, 2023

False positives and negatives in bioanalytical assays remain a persistent problem. Herein, multifunctional photoelectrochemical (PEC) biosensor based on ZnIn2S4 (ZIS)/ZnS quantum dots (QDs)@Au–Ag-reversed photocurrent of Cu-metal–organic framework (MOF) coupled with CRISPR/Cas-12a-shearing was innovatively developed for assay dual targets. First, Cu-MOF as good PEC material shows cathodic photocurrent. Then, numerous ZIS/ZnS QDs were assembled to the Au–Ag nanoparticles (NPs) prepare stable highly amplified signal probe, which can just match energy level Cu-MOFs realized polarity-reversed first time. As empty-core nanostructure NPs has high specific surface area low density, bimetallic nanocrystal much increase reaction rate improve redox efficiency. When target CEA-produced cDNA opened hairpin DNA (HP1 DNA) electrode, QDs@Au–Ag probe conjugated electrode via hybridization, achieving significantly reversed current CEA detection. Moreover, binding kanamycin/aptamer generated acDNA (activator), activate trans-cleavage activity CRISPR-CAS12a system ssDNA, so sheared caused obvious decrease kanamycin The newly displayed excellent properties MOF combined unique CRISPR-Cas12a achieve sensitive detection targets, open new sensing platform rapid accurate analysis multiple targets effectively avoid false results clinical testing.

Язык: Английский

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Novel Ultralow-Potential Electrochemiluminescence Aptasensor for the Highly Sensitive Detection of Zearalenone Using a Resonance Energy Transfer System

Analytical Chemistry, Год журнала: 2023, Номер 95(40), С. 15125 - 15132

Опубликована: Сен. 29, 2023

An ultralow-potential electrochemiluminescence (ECL) aptasensor has been designed for zearalenone (ZEN) assay based on a resonance energy transfer (RET) system with SnS2 QDs/g-C3N4 as novel luminophore and CuO/NH2-UiO-66 dual-quencher. QDs were loaded onto g-C3N4 nanosheets enhanced the ECL luminescence via strong synergistic effects under an ultralow potential. The UV-vis absorption spectrum of exhibits considerable overlap emission QDs/g-C3N4, important consideration RET process. In order to stimulate RET, ZEN aptamer complementary DNA are introduced conjugation between donor acceptor. With binding interaction by its aptamer, is removed from electrode surface, resulting in inhibition increase signal. Under optimal conditions, as-prepared quantified 0.5 μg·mL-1 0.1 fg·mL-1 low limit detection 0.085 fg·mL-1, it exhibited good stability, excellent specificity, high reproducibility, desirable practicality. sensing strategy provides method mycotoxins monitor food safety.

Язык: Английский

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A potential-resolved ratiometric electrochemiluminescence aptasensor for Pb2+: Gold nanoclusters and amino-terminated perylene derivative as both emitters and resonance energy transfer donor-acceptor pair

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 386, С. 133758 - 133758

Опубликована: Март 30, 2023

Язык: Английский

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Portable biosensor for on-site detection of kanamycin in water samples based on CRISPR-Cas12a and an off-the-shelf glucometer

The Science of The Total Environment, Год журнала: 2023, Номер 872, С. 162279 - 162279

Опубликована: Фев. 16, 2023

Язык: Английский

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Zn²⁺-Induced Gold Cluster Aggregation Enhanced Electrochemiluminescence for Ultrasensitive Detection of MicroRNA-21

Analytical Chemistry, Год журнала: 2023, Номер 95(13), С. 5568 - 5574

Опубликована: Март 22, 2023

Herein, Zn2+-induced gold cluster aggregation (Zn2+-GCA) as a high-efficiency electrochemiluminescence (ECL) emitter is first employed to construct an ECL biosensor ultrasensitively detect microRNA-21 (miRNA-21). Impressively, Zn2+ not only can induce the of monodispersed clusters (Au NCs) limit ligand vibration Au NCs for improving emission but also be utilized coreaction accelerator catalyze dissociation coreactant S2O82– into sulfate radicals (SO4•–) improve interaction efficiency between Zn2+-GCA and S2O82–, resulting in further intense emission. Compared stabilized by bovine serum albumin with 0.40%, possessed high 10.54%, regarding [Ru(bpy)3]2+/S2O82– system standard. Furthermore, output DNA modified poly adenine (polyA) obtained from enzyme-free target recycling amplification efficiently immobilized on surface nanoparticles NPs) reduce defect special design, cumbersome operation, low stability. Thus, ultrasensitive based Zn2+-GCA/S2O82– achieved detection miRNA-21 44.7 aM. This strategy presents new idea design highly efficient emitters, which expected used field bioanalysis clinical diagnosis.

Язык: Английский

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AuPt Nanodonuts as a Novel Coreaction Accelerator and Luminophore for a Label-free Electrochemiluminescence Aptasensor

Analytical Chemistry, Год журнала: 2023, Номер 95(37), С. 13838 - 13843

Опубликована: Авг. 31, 2023

Novel and effective coreaction accelerators are of great importance in electrochemiluminescence (ECL) systems. In this work, novel AuPt nanodonuts, i.e., SnS2 quantum dots (QDs)/Cys-AuPt heterogeneous nanorings (NRs), serve as both a highly accelerator the luminophore label-free ECL aptasensor. The nanodonuts were formed by decorating QDs onto NR surfaces, which would promote production more coreactant intermediate QDs/K2S2O8 system. As result, performance was greatly improved. Meanwhile, l-cysteine (l-Cys) played an important role combination between NRs QDs, served matrix to load numerous lincomycin (Lin) aptamers. Under optimal conditions, aptasensor exhibited ultrasensitive detection Lin from 1 fg/mL 0.1 pg/mL with limit (LOD) 0.7 (1.72 fM).

Язык: Английский

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MOF-Derived Cu-BTC Nanowire-Embedded 2D Leaf-like Structured ZIF Composite-Based Aptamer Sensors for Real-Time In Vivo Insulin Monitoring

ACS Applied Materials & Interfaces, Год журнала: 2022, Номер 14(25), С. 28639 - 28650

Опубликована: Июнь 16, 2022

Insulin, which is a hormone produced by the β-cells of pancreas, regulates glucose levels in blood and can transport into cells to produce glycogen or triglycerides. Insulin deficiency lead hyperglycemia diabetes. Therefore, insulin detection critical clinical diagnosis. In this study, disposable Au electrodes were modified with copper(II) benzene-1,3,5-tricarboxylate (Cu-BTC)/leaf-like zeolitic imidazolate framework (ZIF-L) for detection. The aptamers are easily immobilized on Cu-BTC/ZIF-L composite physical adsorption facilitated specific interaction between insulin. composite-based aptasensor presented wide linear range (0.1 pM 5 μM) low limit 0.027 pM. addition, displayed high specificity, good reproducibility stability, favorable practicability human serum samples. For vivo tests, composite-modified implanted non-diabetic diabetic mice, was quantified using electrochemical enzyme-linked immunosorbent assay methods.

Язык: Английский

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Bimetallic Metal–Organic Frameworks as an Efficient Capture Probe in Signal On–Off–On Electrochemiluminescence Aptasensor for Microcystin-LR Detection

Analytical Chemistry, Год журнала: 2023, Номер 95(22), С. 8487 - 8495

Опубликована: Май 22, 2023

To ensure drinking water quality, the development of rapid and accurate analytical methods is essential. Herein, a highly sensitive electrochemiluminescence (ECL) aptasensor-based on signal on–off–on strategy was developed to detect pollutant microcystin-LR (MC-LR). This based newly prepared ruthenium-copper metal–organic framework (RuCu MOF) as ECL signal-transmitting probe three types PdPt alloy core–shell nanocrystals with different crystal structures signal-off probes. Compounding copper-based MOF (Cu-MOF) precursor ruthenium bipyridyl at room temperature facilitated retention intrinsic crystallinity high porosity MOFs well afforded excellent performance. Since bipyridine in RuCu could transfer energies organic ligand (H3BTC), ultra-efficient luminescent finally obtained, which greatly improved sensitivity aptasensor. further improve aptasensor, quenching effects noble metal nanoalloy particles states were investigated, contained octahedral (PdPtOct), rhombic dodecahedral (PdPtRD), nanocube (PdPtNC). Among them, PdPtRD nanocrystal exhibited higher activity durability, stemming from charge redistribution caused by hybridization Pt Pd atoms. Moreover, also load more −NH2–DNA strands because it exposed active sites large specific surface area. The fabricated aptasensor outstanding stability MC-LR detection, linear detection range 0.0001–50 ng mL–1. study provides valuable directions for application nanoparticles metals bimetallic field immunoassay.

Язык: Английский

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Catalytic degradation of tetracycline using marigold flower-like structure erbium molybdate decorated on sulphur-doped g-C3N4 nanocomposite: Kinetics, thermodynamics, DFT calculations, and toxicity studies

Separation and Purification Technology, Год журнала: 2023, Номер 330, С. 125439 - 125439

Опубликована: Окт. 20, 2023

Язык: Английский

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