Toehold Strand Displacement-Mediated Exponential HCR for Highly Sensitive and Specific Analysis of miRNA in Living Cells
Analytical Chemistry,
Год журнала:
2024,
Номер
96(22), С. 9078 - 9087
Опубликована: Май 21, 2024
As
an
important
disease
biomarker,
the
development
of
sensitive
detection
strategies
for
miRNA,
especially
intracellular
miRNA
imaging
strategies,
is
helpful
early
diagnosis
diseases,
pathological
research,
and
drug
development.
Hybridization
chain
reaction
(HCR)
widely
used
analysis
because
its
high
specificity
lack
biological
enzymes.
However,
classic
HCR
exhibits
linear
amplification
with
low
efficiency,
limiting
use
rapid
trace
in
living
cells.
To
address
this
problem,
we
proposed
a
toehold-mediated
exponential
(TEHCR)
to
achieve
highly
efficient
cells
using
β-FeOOH
nanoparticles
as
transfection
vectors.
The
limit
TEHCR
was
92.7
fM,
which
8.8
×
103
times
lower
compared
traditional
HCR,
it
can
effectively
distinguish
single-base
mismatch
specificity.
also
different
expression
levels
cancer
normal
Furthermore,
be
construct
OR
logic
gates
dual
without
need
additional
probes,
demonstrating
flexibility.
This
method
expected
play
role
clinical
miRNA-related
well
promote
gates.
Язык: Английский
Spherical nucleic acids: emerging amplifiers for therapeutic nanoplatforms
Nanoscale,
Год журнала:
2024,
Номер
16(9), С. 4392 - 4406
Опубликована: Янв. 1, 2024
This
review
summarizes
the
amplification
effect
of
SNAs
in
various
combined
therapy
treatment
strategies
and
highlights
recent
advances
clinical
translation.
Язык: Английский
Tumor Microenvironment-Responsive Lipid Peroxidation Amplifier: Harnessing Ferroptosis Resistance to Devastate the Ferroptosis Defense System
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 26, 2025
Amplification
of
lipid
peroxidation
with
tumor
specificity
represents
a
new
avenue
to
boost
ferroptosis-mediated
anticancer
therapeutics
but
remains
challenging.
Herein,
we
proposed
metal-phenolic-network
(MPN)-coated
nanohybrid
as
microenvironment-responsive
amplifier,
consisting
reactive
oxygen
species
generator
MPN,
glutathione
(GSH)
scavenger
GSH-P,
and
peroxidase
4
(GPX4)
mRNA
gene
silencing
sequence.
The
protective
MPN
shell
this
amplifier
can
be
specifically
disintegrated
by
acidic
adenosine
triphosphate
(ATP)-rich
microenvironments
induce
oxidative
stress
through
the
dual
disruption
redox
homeostasis
(Fenton-catalytic
accumulation
GSH
depletion).
Furthermore,
stress-induced
upregulation
ferroptosis
resistance-related
apurinic/apyrimidinic
endonuclease
1
(APE1)
is
further
ingeniously
employed
an
amplification
element
prompt
release
(AP)
site-embedded
GPX4
sequence
which
downregulate
level.
Based
on
tandem
depletion
substrate
GPX4,
defense
system
GPX4/GSH
heavily
devastated
enable
for
effectively
improving
efficiency.
We
expect
strategy
expanded
other
important
regulatory
proteins
provide
mechanism
study
therapy.
Язык: Английский
Smart Stimuli‐Responsive Spherical Nucleic Acids: Cutting‐Edge Platforms for Biosensing, Bioimaging, and Therapeutics
Small,
Год журнала:
2024,
Номер
20(24)
Опубликована: Фев. 1, 2024
Abstract
Spherical
nucleic
acids
(SNAs)
with
exceptional
colloidal
stability,
multiple
modularity,
and
programmability
are
excellent
candidates
to
address
common
molecular
delivery‐related
issues.
Based
on
this,
the
higher
targeting
accuracy
enhanced
controllability
of
stimuli‐responsive
SNAs
render
them
precise
nanoplatforms
inestimable
prospects
for
diverse
biomedical
applications.
Therefore,
tailored
diagnosis
treatment
may
be
a
robust
strategy
break
through
bottlenecks
associated
traditional
nanocarriers.
Various
engineered
incorporation
multifunctional
modifications
meet
demands
development
acid
functionalization.
This
review
provides
comprehensive
overview
prominent
research
in
this
area
recent
advancements
utilization
biosensing,
bioimaging,
therapeutics.
For
each
aspect,
SNA
that
exhibit
responsive
behavior
both
internal
stimuli
(including
sequence,
enzyme,
redox
reactions,
pH)
external
(such
as
light
temperature)
highlighted.
is
expected
offer
inspiration
guidance
strategies
rational
design
field
biomedicine.
Язык: Английский
Synergy of Target-Induced Magnetic Network and Single-Drop Chromogenic System for Ultrasensitive “All-in-Tube” Detection of miRNA in Whole Blood
Yao Yao,
Wei Liu,
Jianping Guan
и другие.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(29), С. 12012 - 12021
Опубликована: Июль 8, 2024
The
development
of
liquid
biopsy
methods
for
the
accurate
and
reliable
detection
miRNAs
in
whole
blood
is
critical
early
diagnosis
monitoring
diseases.
However,
quantification
miRNA
expression
levels
remains
challenging
due
to
complex
matrix
low
abundance
samples.
Herein,
we
report
a
contactless
signal
output
strategy
with
background
interference
that
ensures
"zero-contact"
between
reaction
system
colorimetry
system.
designed
target-induced
magnetic
ZnS/ZIF-90/ZnS
network
can
serve
as
unique
amplifier
transducer.
It
releases
hydrogen
sulfide
(H
Язык: Английский
Biomarker Multiplexing with Rational Design of Nucleic Acid Probe Complex
Yu Zhao,
Hui Xin,
Chunyan Wang
и другие.
Analysis & Sensing,
Год журнала:
2024,
Номер
4(5)
Опубликована: Июль 10, 2024
Abstract
The
expression
profiles
of
intracellular
biomarkers
hold
significance
for
understanding
cellular
biological
functions
and
tracking
pathological
activities.
Due
to
its
programmability
biocompatibility,
extensive
efforts
have
been
devoted
design
various
kinds
nucleic
acid
probes
biomarker
detection.
However,
pinpointing
a
single
could
end
up
in
false
positive
signal,
delaying
diagnosis.
In
this
review,
we
present
an
overview
current
advances
detection
signal
amplification
techniques.
We
highlight
strategies
multiplexing
with
combination
isothermal
approaches.
High
specificity
sensitivity
are
the
two
criteria
desired
probe,
as
challenges
encountered
by
probe
that
operates
efficiently
systems.
With
higher
identification
accuracy,
may
be
able
move
one
step
closer
precision
medicine.
Язык: Английский
π–π Stacking Network-Based Supramolecular Peptide Nanoprobe for Visualization of the ICB-Enhanced Ferroptosis Process
Analytical Chemistry,
Год журнала:
2024,
Номер
96(32), С. 13317 - 13325
Опубликована: Июль 31, 2024
The
construction
of
coassembled
peptide
nanoprobes
based
on
structural
adaptation
provides
an
effective
template
for
stable
monitoring
the
molecular
events
in
physiological
and
pathological
processes.
This
also
greatly
expands
their
applications
biomedicine,
such
as
multimodal
combined
diagnosis
treatment.
However,
insufficient
understanding
physicochemical
properties
features
different
molecules
still
makes
it
difficult
to
construct
probes
with
mutually
reinforcing
functions,
leading
unpredictable
effects.
Here,
we
showed
how
utilize
π-π
stacking
network
β-sheets
formed
by
PD-L1-targeting
peptides
capture
small
ferroptosis
thus,
coassembling
them
into
a
visual
probe
synergistic
Compared
individual
components,
strategy
could
significantly
improve
stability
nanoprobe,
inducing
stronger
effects
immune
checkpoint
blocking
effects,
track
reflect
process.
study
new
insights
design
multicomponent
collaborative
coassembly
systems
biological
Язык: Английский
Spherical Nucleic Acids for Biomedical Applications
Advanced Sensor and Energy Materials,
Год журнала:
2024,
Номер
3(4), С. 100117 - 100117
Опубликована: Июль 30, 2024
Spherical
nucleic
acids
(SNAs)
are
a
3D
spherical
nanostructure
composed
of
highly
oriented,
dense
layers
oligonucleotides
conjugated
to
hollow
or
solid
core.
This
structure
allows
SNAs
show
resistance
nuclease
degradation,
enter
into
nearly
all
cells
without
transfection
agents
and
enable
precise
interactions
with
target
molecules.
Based
on
superior
biological
properties,
can
be
tailored
for
diverse
applications,
rendering
them
flexible
biosafe
tool
applications
as
well
an
enabling
platform
therapy.
In
this
review,
we
mainly
discuss
the
conjugation
mode
focus
recent
advances
in
their
such
biomedical
detection,
imaging,
drug
delivery.
Finally,
remaining
challenges
future
directions
also
discussed
proposed.
Язык: Английский
Viewpoints about the Application of Nanoprobe Technology in Orthopedic Diseases Based on Its Functions in Disease Diagnosis and Treatment
Nano Biomedicine and Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Enzyme‐Programmed Self‐Assembly of Nanoparticles
ChemBioChem,
Год журнала:
2024,
Номер
25(15)
Опубликована: Май 31, 2024
Nanoparticles
are
a
hot
topic
in
the
field
of
nanomaterial
research
due
to
their
excellent
physical
and
chemical
properties.
In
recent
years,
DNA-directed
nanoparticle
self-assembly
technology
has
been
widely
applied
development
numerous
complex
superstructures.
Due
inherent
stability
surface
electric
repulsion
nanoparticles,
it
is
difficult
make
superstructures
respond
molecular
signals
external
environment.
fact,
enzyme-programmed
systems
developed
allow
diverse
functions,
including
logical
operations,
signal
amplification,
dynamic
assembly
control.
Therefore,
combining
enzyme-controlled
DNA
may
endow
with
more
flexibility
program
design,
allowing
them
variety
signals.
this
review,
we
summarize
basic
principles
DNA/nanoparticle
introduce
its
applications
heavy
metal
detection,
gene
expression,
proteins
inside
living
cells,
cancer
cell
therapy,
drug
delivery.
With
continuous
new
materials
increasing
functionality
enzyme
circuits,
enzyme-directed
self-assembled
probe
expected
see
significant
future
development.
Язык: Английский