Toehold Strand Displacement-Mediated Exponential HCR for Highly Sensitive and Specific Analysis of miRNA in Living Cells

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(22), P. 9078 - 9087

Published: May 21, 2024

As an important disease biomarker, the development of sensitive detection strategies for miRNA, especially intracellular miRNA imaging strategies, is helpful early diagnosis diseases, pathological research, and drug development. Hybridization chain reaction (HCR) widely used analysis because its high specificity lack biological enzymes. However, classic HCR exhibits linear amplification with low efficiency, limiting use rapid trace in living cells. To address this problem, we proposed a toehold-mediated exponential (TEHCR) to achieve highly efficient cells using β-FeOOH nanoparticles as transfection vectors. The limit TEHCR was 92.7 fM, which 8.8 × 103 times lower compared traditional HCR, it can effectively distinguish single-base mismatch specificity. also different expression levels cancer normal Furthermore, be construct OR logic gates dual without need additional probes, demonstrating flexibility. This method expected play role clinical miRNA-related well promote gates.

Language: Английский

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Spherical nucleic acids: emerging amplifiers for therapeutic nanoplatforms

Nanoscale, Journal Year: 2024, Volume and Issue: 16(9), P. 4392 - 4406

Published: Jan. 1, 2024

This review summarizes the amplification effect of SNAs in various combined therapy treatment strategies and highlights recent advances clinical translation.

Language: Английский

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Tumor Microenvironment-Responsive Lipid Peroxidation Amplifier: Harnessing Ferroptosis Resistance to Devastate the Ferroptosis Defense System

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Amplification of lipid peroxidation with tumor specificity represents a new avenue to boost ferroptosis-mediated anticancer therapeutics but remains challenging. Herein, we proposed metal-phenolic-network (MPN)-coated nanohybrid as microenvironment-responsive amplifier, consisting reactive oxygen species generator MPN, glutathione (GSH) scavenger GSH-P, and peroxidase 4 (GPX4) mRNA gene silencing sequence. The protective MPN shell this amplifier can be specifically disintegrated by acidic adenosine triphosphate (ATP)-rich microenvironments induce oxidative stress through the dual disruption redox homeostasis (Fenton-catalytic accumulation GSH depletion). Furthermore, stress-induced upregulation ferroptosis resistance-related apurinic/apyrimidinic endonuclease 1 (APE1) is further ingeniously employed an amplification element prompt release (AP) site-embedded GPX4 sequence which downregulate level. Based on tandem depletion substrate GPX4, defense system GPX4/GSH heavily devastated enable for effectively improving efficiency. We expect strategy expanded other important regulatory proteins provide mechanism study therapy.

Language: Английский

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Smart Stimuli‐Responsive Spherical Nucleic Acids: Cutting‐Edge Platforms for Biosensing, Bioimaging, and Therapeutics

Small, Journal Year: 2024, Volume and Issue: 20(24)

Published: Feb. 1, 2024

Abstract Spherical nucleic acids (SNAs) with exceptional colloidal stability, multiple modularity, and programmability are excellent candidates to address common molecular delivery‐related issues. Based on this, the higher targeting accuracy enhanced controllability of stimuli‐responsive SNAs render them precise nanoplatforms inestimable prospects for diverse biomedical applications. Therefore, tailored diagnosis treatment may be a robust strategy break through bottlenecks associated traditional nanocarriers. Various engineered incorporation multifunctional modifications meet demands development acid functionalization. This review provides comprehensive overview prominent research in this area recent advancements utilization biosensing, bioimaging, therapeutics. For each aspect, SNA that exhibit responsive behavior both internal stimuli (including sequence, enzyme, redox reactions, pH) external (such as light temperature) highlighted. is expected offer inspiration guidance strategies rational design field biomedicine.

Language: Английский

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Synergy of Target-Induced Magnetic Network and Single-Drop Chromogenic System for Ultrasensitive “All-in-Tube” Detection of miRNA in Whole Blood

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(29), P. 12012 - 12021

Published: July 8, 2024

The development of liquid biopsy methods for the accurate and reliable detection miRNAs in whole blood is critical early diagnosis monitoring diseases. However, quantification miRNA expression levels remains challenging due to complex matrix low abundance samples. Herein, we report a contactless signal output strategy with background interference that ensures "zero-contact" between reaction system colorimetry system. designed target-induced magnetic ZnS/ZIF-90/ZnS network can serve as unique amplifier transducer. It releases hydrogen sulfide (H

Language: Английский

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Biomarker Multiplexing with Rational Design of Nucleic Acid Probe Complex

Analysis & Sensing, Journal Year: 2024, Volume and Issue: 4(5)

Published: July 10, 2024

Abstract The expression profiles of intracellular biomarkers hold significance for understanding cellular biological functions and tracking pathological activities. Due to its programmability biocompatibility, extensive efforts have been devoted design various kinds nucleic acid probes biomarker detection. However, pinpointing a single could end up in false positive signal, delaying diagnosis. In this review, we present an overview current advances detection signal amplification techniques. We highlight strategies multiplexing with combination isothermal approaches. High specificity sensitivity are the two criteria desired probe, as challenges encountered by probe that operates efficiently systems. With higher identification accuracy, may be able move one step closer precision medicine.

Language: Английский

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π–π Stacking Network-Based Supramolecular Peptide Nanoprobe for Visualization of the ICB-Enhanced Ferroptosis Process

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(32), P. 13317 - 13325

Published: July 31, 2024

The construction of coassembled peptide nanoprobes based on structural adaptation provides an effective template for stable monitoring the molecular events in physiological and pathological processes. This also greatly expands their applications biomedicine, such as multimodal combined diagnosis treatment. However, insufficient understanding physicochemical properties features different molecules still makes it difficult to construct probes with mutually reinforcing functions, leading unpredictable effects. Here, we showed how utilize π-π stacking network β-sheets formed by PD-L1-targeting peptides capture small ferroptosis thus, coassembling them into a visual probe synergistic Compared individual components, strategy could significantly improve stability nanoprobe, inducing stronger effects immune checkpoint blocking effects, track reflect process. study new insights design multicomponent collaborative coassembly systems biological

Language: Английский

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Spherical Nucleic Acids for Biomedical Applications

Advanced Sensor and Energy Materials, Journal Year: 2024, Volume and Issue: 3(4), P. 100117 - 100117

Published: July 30, 2024

Spherical nucleic acids (SNAs) are a 3D spherical nanostructure composed of highly oriented, dense layers oligonucleotides conjugated to hollow or solid core. This structure allows SNAs show resistance nuclease degradation, enter into nearly all cells without transfection agents and enable precise interactions with target molecules. Based on superior biological properties, can be tailored for diverse applications, rendering them flexible biosafe tool applications as well an enabling platform therapy. In this review, we mainly discuss the conjugation mode focus recent advances in their such biomedical detection, imaging, drug delivery. Finally, remaining challenges future directions also discussed proposed.

Language: Английский

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Viewpoints about the Application of Nanoprobe Technology in Orthopedic Diseases Based on Its Functions in Disease Diagnosis and Treatment

Nano Biomedicine and Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

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Enzyme‐Programmed Self‐Assembly of Nanoparticles

ChemBioChem, Journal Year: 2024, Volume and Issue: 25(15)

Published: May 31, 2024

Nanoparticles are a hot topic in the field of nanomaterial research due to their excellent physical and chemical properties. In recent years, DNA-directed nanoparticle self-assembly technology has been widely applied development numerous complex superstructures. Due inherent stability surface electric repulsion nanoparticles, it is difficult make superstructures respond molecular signals external environment. fact, enzyme-programmed systems developed allow diverse functions, including logical operations, signal amplification, dynamic assembly control. Therefore, combining enzyme-controlled DNA may endow with more flexibility program design, allowing them variety signals. this review, we summarize basic principles DNA/nanoparticle introduce its applications heavy metal detection, gene expression, proteins inside living cells, cancer cell therapy, drug delivery. With continuous new materials increasing functionality enzyme circuits, enzyme-directed self-assembled probe expected see significant future development.

Language: Английский

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