Engineering DNA Nanodevices with Multi-site Recognition and Multi-signal Output for Accurate Intracellular LncRNA Imaging

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Dynamic DNA nanodevices, known for their high programmability and controllability, are pivotal in intracellular biomarker imaging. However, these nanodevices often suffer from inadequate detection sensitivity specificity due to limited cellular loading capacity low signal feedback. Herein, we engineered an integrated multi-site recognition multi-signal output of four-leaf clover dynamic nanodevice (MEMORY) that enables sensitive accurate long noncoding RNA (lncRNA) MEMORY features one fluorophore (FAM)-modified cross-shaped structure as spatial-confinement scaffolds loaded with four identical quenchers (BHQ1)-modified probes (RPs), ensuring a background initially. In the presence target lncRNA, multiple sites facilitate hybridization selectively release RPs, exposing toehold region outputting green fluorescence (FAM) signal. Furthermore, exposed can trigger efficient rapid chain reaction (HCR) amplification, red (Cy5) MEMORY's increase likelihood collisions, enhancing efficiency, while its provides sequential feedback through FAM Cy5, boosting overall intensity. With lncRNA metastasis-related lung adenocarcinoma transcript 1 (MALAT1) model, offers linear range pM 100 nM, limit 0.29 pM. We demonstrated differentiate between normal tumor cells based on MALAT1 This will offer valuable tools imaging biomarkers.

Язык: Английский

---

The Trend of Nonenzymatic Nucleic Acid Amplification: Strategies and Diagnostic Application

Precision Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Язык: Английский

---

Spatially Controlled MicroRNA Imaging in Mitochondria via Enzymatic Activation of Hybridization Chain Reaction

Small Methods, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 14, 2024

Abstract Live‐cell imaging of RNA in specific subcellular compartments is essential for elucidating the rich repertoire cellular functions, but it has been limited by a lack simple, precisely controlled methods. Here such an approach presented via combination hybridization chain reaction and spatially restricted enzymatic activation with organelle‐targeted delivery. The system can localize engineered DNA hairpins mitochondria, where target RNA‐initiated events selectively activated enzyme, enabling amplified high precision. It demonstrated that compatible live cell visualization enables regulatable microRNA mitochondria. Since situ signal amplification enzyme eliminates need genetically encoded protein overexpression, envisioned this simple platform will be broadly applicable precise resolution variety biological processes.

Язык: Английский

---

Turning Waste into Wealth: Enzyme-Activated DNA Sensor Based on Reactant Recycle for Spatially Selective Imaging MicroRNA toward Target Cells

Analytica Chimica Acta, Год журнала: 2024, Номер 1337, С. 343557 - 343557

Опубликована: Дек. 16, 2024

Язык: Английский

---

An electrochemical aptasensor for the detection of bisphenol A based on triple signal amplification assisted by gold nanoparticles, hemin/G-quadruplex DNAzyme, and exonuclease I

Microchimica Acta, Год журнала: 2024, Номер 192(1)

Опубликована: Дек. 22, 2024

Язык: Английский

---