An electrochemical aptasensor for the detection of bisphenol A based on triple signal amplification assisted by gold nanoparticles, hemin/G-quadruplex DNAzyme, and exonuclease I DOI
Yanhong Liu,

Xueqin Xu

Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)

Published: Dec. 22, 2024

Language: Английский

Engineering DNA Nanodevices with Multi-site Recognition and Multi-signal Output for Accurate Intracellular LncRNA Imaging DOI
Jingyi Tang,

Mei-Ling Zhao,

Xuemei Zhou

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Dynamic DNA nanodevices, known for their high programmability and controllability, are pivotal in intracellular biomarker imaging. However, these nanodevices often suffer from inadequate detection sensitivity specificity due to limited cellular loading capacity low signal feedback. Herein, we engineered an integrated multi-site recognition multi-signal output of four-leaf clover dynamic nanodevice (MEMORY) that enables sensitive accurate long noncoding RNA (lncRNA) MEMORY features one fluorophore (FAM)-modified cross-shaped structure as spatial-confinement scaffolds loaded with four identical quenchers (BHQ1)-modified probes (RPs), ensuring a background initially. In the presence target lncRNA, multiple sites facilitate hybridization selectively release RPs, exposing toehold region outputting green fluorescence (FAM) signal. Furthermore, exposed can trigger efficient rapid chain reaction (HCR) amplification, red (Cy5) MEMORY's increase likelihood collisions, enhancing efficiency, while its provides sequential feedback through FAM Cy5, boosting overall intensity. With lncRNA metastasis-related lung adenocarcinoma transcript 1 (MALAT1) model, offers linear range pM 100 nM, limit 0.29 pM. We demonstrated differentiate between normal tumor cells based on MALAT1 This will offer valuable tools imaging biomarkers.

Language: Английский

Citations

0
The Trend of Nonenzymatic Nucleic Acid Amplification: Strategies and Diagnostic Application DOI Creative Commons
Junyou Li,

Ting Li,

Zheng Zou

et al.

Precision Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

0
Spatially Controlled MicroRNA Imaging in Mitochondria via Enzymatic Activation of Hybridization Chain Reaction DOI Open Access

Kaining Dai,

Jian Zhao, Lele Li

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Live‐cell imaging of RNA in specific subcellular compartments is essential for elucidating the rich repertoire cellular functions, but it has been limited by a lack simple, precisely controlled methods. Here such an approach presented via combination hybridization chain reaction and spatially restricted enzymatic activation with organelle‐targeted delivery. The system can localize engineered DNA hairpins mitochondria, where target RNA‐initiated events selectively activated enzyme, enabling amplified high precision. It demonstrated that compatible live cell visualization enables regulatable microRNA mitochondria. Since situ signal amplification enzyme eliminates need genetically encoded protein overexpression, envisioned this simple platform will be broadly applicable precise resolution variety biological processes.

Language: Английский

Citations

1
Turning Waste into Wealth: Enzyme-Activated DNA Sensor Based on Reactant Recycle for Spatially Selective Imaging MicroRNA toward Target Cells DOI
Yaokun Xia,

Guomin Ju,

Yongting Zhou

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1337, P. 343557 - 343557

Published: Dec. 16, 2024

Language: Английский

Citations

0
An electrochemical aptasensor for the detection of bisphenol A based on triple signal amplification assisted by gold nanoparticles, hemin/G-quadruplex DNAzyme, and exonuclease I DOI
Yanhong Liu,

Xueqin Xu

Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)

Published: Dec. 22, 2024

Language: Английский

Citations

0