JACS Au,
Год журнала:
2022,
Номер
2(11), С. 2381 - 2399
Опубликована: Окт. 21, 2022
Artificial
molecular
machines
have
found
widespread
applications
ranging
from
fundamental
studies
to
biomedicine.
More
recent
advances
in
exploiting
unique
physical
and
chemical
properties
of
DNA
led
the
development
DNA-based
artificial
machines.
The
unprecedented
programmability
provides
a
powerful
means
design
complex
sophisticated
that
can
exert
mechanical
force
or
motion
realize
tasks
controllable,
modular
fashion.
This
Perspective
highlights
potential
strategies
construct
using
double-stranded
DNA,
functional
nucleic
acids,
frameworks,
which
enable
improved
control
over
reaction
pathways
behaviors.
We
also
outline
challenges
opportunities
for
biophysics,
biosensing,
biocomputing.
Abstract
The
ability
to
design
nanostructures
with
arbitrary
shapes
and
controllable
motions
has
made
DNA
nanomaterials
used
widely
construct
diverse
nanomachines
various
structures
functions.
exhibit
excellent
properties,
including
programmability,
stability,
biocompatibility,
can
be
modified
different
functional
groups.
Among
these
nanoscale
architectures,
walker
is
one
of
the
most
popular
nanodevices
ingenious
flexible
function.
In
past
several
years,
walkers
have
amazing
progress
ranging
from
structural
biological
applications
constructing
biosensors
for
detection
cancer‐associated
biomarkers.
this
review,
key
driving
forces
are
first
summarized.
Then,
numbers
legs
introduced.
Furthermore,
biosensing
detection‐
nucleic
acids,
proteins,
ions,
bacteria
Finally,
new
frontiers
opportunities
developing
walker‐based
discussed.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(23), С. 8277 - 8284
Опубликована: Май 30, 2022
CRISPR/Cas12,
a
highly
efficient
and
specific
nucleic
acid
recognition
system,
has
been
broadly
employed
to
detect
amplified
DNA
products.
However,
most
reported
methods
adopt
two-step
detection
mode
that
needs
liquid
transfer
step,
thus
complicating
the
procedure
posing
risk
of
aerosol
contamination.
A
one-pot
method
can
obviate
these
problems,
but
it
suffers
from
poor
efficiency
due
loss
amplification
templates
elicited
by
CRISPR/Cas12
cleavage.
In
this
study,
we
discovered
glycerol
additive
dramatically
promoted
recombinase
polymerase
(RPA)-CRISPR/Cas12a
method.
Compared
with
glycerol-free
version,
its
sensitivity
was
nearly
100-fold
higher
close
canonical
Further
investigation
displayed
enhanced
attributed
phase
separation
RPA
CRISPR/Cas12a
system
during
initial
reaction
caused
viscosity.
This
triumphantly
harnessed
for
African
swine
fever
virus
(ASFV)
SARS-CoV-2,
achieving
naked-eye
readout
through
smartphone-equipped
device.
The
currently
developed
glycerol-enhanced
RPA-CRISPR/Cas12a
be
an
advantageous
point-of-care
platform
on
account
simplicity,
high
sensitivity,
universality.
Journal of the American Chemical Society,
Год журнала:
2022,
Номер
144(30), С. 13475 - 13486
Опубликована: Июль 8, 2022
Breast
cancer
is
very
heterogeneous
and
the
most
frequently
diagnosed
worldwide,
precise
therapy
targeting
specific
subtypes
may
improve
survival
rates
of
breast
patients.
In
this
study,
we
designed
a
biomimetic
vesicle
by
camouflaging
catalytic
DNA
machinery
with
cell
membrane,
which
enabled
molecular
classification
circulating
exosomes
for
subtype-based
diagnosis
through
homotypic
recognition.
addition,
vesicles
specifically
targeted
fused
phenotypic
homology
manipulated
to
amplify
electrochemical
signaling
using
exosomal
RNA
as
an
endogenous
trigger.
The
prepared
MCF-7
cell-derived
membranes
were
shown
recognize
estrogen
receptor-positive
exhibited
low
detection
limit
557
particles
mL–1
microRNA-375
used
biomarker.
Furthermore,
MDA-MB-231
displayed
satisfactory
performance
in
analysis
triple-negative
potential
therapeutic
target,
PD-L1
mRNA,
Most
importantly,
cross-validation
experiments
confirmed
high
accuracy
selectivity
recognition-driven
subtyping
cancer.
When
applied
clinical
samples
patients,
demonstrated
feasibility
reliability
evaluating
features
stage-specific
monitoring
patients
because
signals
showed
positive
correlation
disease
progression.
Therefore,
work
provide
new
ideas
personalized
treatment
throughout
whole
process.
Chemical Science,
Год журнала:
2022,
Номер
13(10), С. 2857 - 2876
Опубликована: Янв. 1, 2022
POC
diagnostics
are
driven
by
the
rapid
advances
in
CRISPR,
electrochemical
and
optical
biosensors.
Related
emerging
strategies
described
discussed
from
perspective
of
facilitating
practical
application
biosensors
testing.
Cell Reports Physical Science,
Год журнала:
2024,
Номер
5(2), С. 101801 - 101801
Опубликована: Янв. 31, 2024
With
recent
advances
in
point-of-care
testing,
wearable
electronics,
and
implantable
medical
devices,
there
is
a
growing
interest
developing
state-of-the-art
methods
to
monitor
human
health
conveniently
dynamically.
Among
these
methods,
electrochemical
biosensors
have
offered
unique
opportunity
transform
lab-in-hospital
lab-around-body
lab-on-body.
In
an
biosensing
system,
appropriate
bioreceptors
that
could
specifically
recognize
capture
target
molecules
pose
significant
impact
on
the
performance
of
biosensors.
Thus,
this
review
systematically
explores
different
types
their
immobilization
utilized
for
detection
wide
range
biomarkers
clinical
applications.
Moreover,
it
delves
into
principles
behind
operation
provides
insights
challenges
limitations
associated
with
use
practical
settings.
Furthermore,
proposes
possible
strategies
overcome
existing
order
stimulate
further
research
implementation
larger
scale.
Angewandte Chemie International Edition,
Год журнала:
2022,
Номер
61(50)
Опубликована: Сен. 30, 2022
Nucleic
acids
are
remarkable
molecules.
In
addition
to
Watson-Crick
base
pairing,
the
different
structural
motifs
of
these
molecules
can
bind
non-nucleic
acid
targets
or
catalyze
chemical
reactions.
Additionally,
nucleic
easily
modified
with
functional
groups.
These
properties
make
acids,
particularly
DNA,
ideally
suited
for
use
in
electrochemical
biosensors,
both
as
biorecognition
elements
and
redox
reporter
probes.
this
Minireview,
we
will
review
historical
evolution
probes
biosensors.
We
then
specific
examples
nucleic-acid-based
biosensors
that
have
been
evaluated
clinical
areas
infectious
disease,
cancer,
cardiovascular
health.
