Benzimidazole Fungicide Carbendazim Induces Gut Inflammation through the TLR5/NF-κB Pathway in Grass Carp

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Fungicides have been increasingly used across various sectors, including agriculture and textiles. The biocidal properties of fungicides may negatively impact the stability intestinal microbiota, thereby posing a threat to health. In this study, we investigated mechanisms damage functional abnormalities in grass carp following 42-day exposure widely fungicide carbendazim at environmentally relevant concentrations (0.2 20 μg/L). Histopathological observations, mRNA protein expression analyses, biochemical analysis, quantification short-chain fatty acids (SCFAs), cytokines, lipopolysaccharide (LPS), 16S ribosomal ribonucleic acid (rRNA), as well internal transcribed spacer (ITS) sequencing, were performed. At concentrations, strongly induced inflammation, leading increased transcriptional translational levels genes involved toll-like receptor five (TLR5)/nuclear factor kappa B (NF-κB) pathway, TLR5, NF-κB, interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα). Additionally, damaged barriers reduced tight junction proteins (e.g., occludin zonula occludens-1/2), goblet cells, immunoglobulin M levels, while also disrupting gut microbiome, metabolic disorders, particularly decreases SCFAs increases LPS. Treatment with TLR5 antagonist TH1020 mitigated inflammation caused by carbendazim, subsequently improving mechanical barrier function. Overall, our findings provide new insights into toxicological underlying carp, indicating that poses significant nontarget organisms. Given its widespread detection environment, these results underscore substantial ecological risks health fish living carbendazim-contaminated water bodies.

Язык: Английский

---

Association of co-exposure to organophosphate esters and per- and polyfluoroalkyl substances and mixture with cardiovascular-kidney-liver-metabolic biomarkers among Chinese adults

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 280, С. 116524 - 116524

Опубликована: Июнь 5, 2024

Organophosphate esters (OPEs) and Per- polyfluoroalkyl substances (PFAS) are ubiquitous environmental contaminants with common exposure sources, leading to their widespread presence in human body. However, evidence on co-exposure OPEs PFAS its impact cardiovascular-kidney-liver-metabolic biomarkers remains limited.

Язык: Английский

---

Tire-wear particle leachate at environmentally relevant concentrations exert a hepatotoxic impact on the black-spotted frog by disrupting the gut–liver axis

Environmental Chemistry and Ecotoxicology, Год журнала: 2024, Номер 6, С. 380 - 389

Опубликована: Янв. 1, 2024

Язык: Английский

---

Distribution and adsorption-desorption of organophosphate esters from land to sea in the sediments of the Beibu Gulf, South China Sea: Impact of seagoing river input

The Science of The Total Environment, Год журнала: 2024, Номер 917, С. 170359 - 170359

Опубликована: Янв. 26, 2024

Язык: Английский

---

Toxic effects of triclosan on hepatic and intestinal lipid accumulation in zebrafish via regulation of m6A-RNA methylation

Aquatic Toxicology, Год журнала: 2024, Номер 269, С. 106884 - 106884

Опубликована: Март 2, 2024

Язык: Английский

---

Microplastics aggravate the bioaccumulation and corresponding food safety risk of antibiotics in edible bivalves by constraining detoxification-related processes

The Science of The Total Environment, Год журнала: 2023, Номер 908, С. 168436 - 168436

Опубликована: Ноя. 8, 2023

Язык: Английский

---

Association between urinary organophosphate ester metabolite exposure and thyroid disease risk among US adults: National Health and Nutrition Examination Survey 2011-2014

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Фев. 9, 2024

Background Organophosphate esters (OPEs) may interfere with thyroid function, but the relationship between OPEs and disease remains unclear. This study aims to elucidate exposure risk in general population United States. Method Data were obtained from 2011-2014 National Health Nutrition Examination Survey cycle. All participants tested for seven OPE metabolites their urine answered questions about whether they had through questionnaires. Logistic regression was employed analyze association individual disease. Weighted Quantile Sum (WQS) modeling utilized assess mixed of Bayesian kernel machine regression(BKMR) models overall effect metabolites. Result A total 2,449 included study, 228 whom a history Bis(1,3-dichloro-2-propyl) phos (BDCPP), Diphenyl phosphate (DPHP) Bis(2-chloroethyl) (BCEP) top three highest detection rates 91.75%, 90.77% 86.57%, respectively. In multivariate logistic models, after adjustment confounding variables, individuals tertile level BCEP significantly positively associated increased (OR=1.57, 95% CI=1.04-2.36), using lowest as reference. positive WQS model, correcting (OR=1.03, CI=1.01-1.06), DPHP having high weights. BKMR not statistically significant, univariate response trends showed that decreased then levels increased. Conclusion The revealed significant an disease, emerging primary contributor. exhibits J-shaped pattern, whereby initially decreases subsequently increases rising exposure. Additional studies are required validate diseases.

Язык: Английский

---

Tris(2-chloroethyl) Phosphate Leads to Unbalanced Circulating Erythrocyte in Mice by Activating both Medullary and Extramedullary Erythropoiesis

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Tris(2-chloroethyl) phosphate (TCEP), a prevalent organophosphorus flame retardant, has been identified in various environmental matrices and human blood samples, provoking alarm regarding its hematological toxicity, subject that not thoroughly investigated. Red cells (RBCs), or erythrocytes, are the predominant cell type peripheral crucial for maintenance of physiological health. This investigation employed oral gavage to examine effects TCEP exposure on erythrocyte counts mice clarify underlying mechanisms. The results demonstrated marked increase circulating RBC post-TCEP exposure, concomitantly heightening risk polycythemia vera (PV). stimulated erythropoiesis across all stages medullary development, including differentiation hematopoietic stem into erythroid progenitors, progression maturation erythrocyte. Moreover, potentiated extramedullary spleen liver. Subsequent bioinformatics analysis implied TCEP-induced was attributed p53 downregulation. Thus, these findings indicate disrupts erythrocyte-mediated homeostasis through enhancement both erythropoiesis, leading alteration equilibrium.

Язык: Английский

---

Degradation of organophosphate flame retardants by white-rot fungi: Degradation pathways and associated toxicity

The Science of The Total Environment, Год журнала: 2025, Номер 959, С. 178260 - 178260

Опубликована: Янв. 1, 2025

The environmental persistence of organophosphate flame retardants (OPFRs) in water is becoming and concern. White Rot Fungi (WRF) have proven its capability to degrade certain OPFRs such as tributyl phosphate (TBP), tris(2-butoxyethyl) (TBEP), tris(2-chloroethyl) (TCEP) tris(2-chloroisopropyl) (TCPP). Despite this capability, there limited knowledge about the specific pathways involved degradation. In study, three different WRF were paired with individual OPFRs, potential transformation products (TPs) identified by UHPLC-HRMS. Some compounds structures further validated NMR. From these data degradation proposed. TBP was degraded successive hydroxylation hydrolysis reactions, a novel dehydrogenation step suggested. Both TCEP TCPP underwent oxidative dechlorination, experiencing subsequent hydrolysis. Uncommon reductive dehalogenation also observed. environmentally relevant methylation. TBEP generated numerous TPs, mainly dealkylations, along hydroxylation. Notably, demethylation proposed for first time. Additional secondary formed through oxidation initial metabolites. Finally, vivo silico toxicity assessments conducted, identifying TPs potentially toxic.

Язык: Английский

---

The isolated Ca-Nx sites in biochar boosting Fe catalyzed Fenton-like oxidation of Tris(2-chloroethyl) phosphate: Properties, mechanisms, and applications

Applied Catalysis B Environment and Energy, Год журнала: 2025, Номер 366, С. 125056 - 125056

Опубликована: Янв. 11, 2025

Язык: Английский

---