Benzimidazole Fungicide Carbendazim Induces Gut Inflammation through the TLR5/NF-κB Pathway in Grass Carp

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Fungicides have been increasingly used across various sectors, including agriculture and textiles. The biocidal properties of fungicides may negatively impact the stability intestinal microbiota, thereby posing a threat to health. In this study, we investigated mechanisms damage functional abnormalities in grass carp following 42-day exposure widely fungicide carbendazim at environmentally relevant concentrations (0.2 20 μg/L). Histopathological observations, mRNA protein expression analyses, biochemical analysis, quantification short-chain fatty acids (SCFAs), cytokines, lipopolysaccharide (LPS), 16S ribosomal ribonucleic acid (rRNA), as well internal transcribed spacer (ITS) sequencing, were performed. At concentrations, strongly induced inflammation, leading increased transcriptional translational levels genes involved toll-like receptor five (TLR5)/nuclear factor kappa B (NF-κB) pathway, TLR5, NF-κB, interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα). Additionally, damaged barriers reduced tight junction proteins (e.g., occludin zonula occludens-1/2), goblet cells, immunoglobulin M levels, while also disrupting gut microbiome, metabolic disorders, particularly decreases SCFAs increases LPS. Treatment with TLR5 antagonist TH1020 mitigated inflammation caused by carbendazim, subsequently improving mechanical barrier function. Overall, our findings provide new insights into toxicological underlying carp, indicating that poses significant nontarget organisms. Given its widespread detection environment, these results underscore substantial ecological risks health fish living carbendazim-contaminated water bodies.

Language: Английский

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Association of co-exposure to organophosphate esters and per- and polyfluoroalkyl substances and mixture with cardiovascular-kidney-liver-metabolic biomarkers among Chinese adults

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 280, P. 116524 - 116524

Published: June 5, 2024

Organophosphate esters (OPEs) and Per- polyfluoroalkyl substances (PFAS) are ubiquitous environmental contaminants with common exposure sources, leading to their widespread presence in human body. However, evidence on co-exposure OPEs PFAS its impact cardiovascular-kidney-liver-metabolic biomarkers remains limited.

Language: Английский

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Tire-wear particle leachate at environmentally relevant concentrations exert a hepatotoxic impact on the black-spotted frog by disrupting the gut–liver axis

Environmental Chemistry and Ecotoxicology, Journal Year: 2024, Volume and Issue: 6, P. 380 - 389

Published: Jan. 1, 2024

Language: Английский

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Distribution and adsorption-desorption of organophosphate esters from land to sea in the sediments of the Beibu Gulf, South China Sea: Impact of seagoing river input

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 917, P. 170359 - 170359

Published: Jan. 26, 2024

Language: Английский

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Toxic effects of triclosan on hepatic and intestinal lipid accumulation in zebrafish via regulation of m6A-RNA methylation

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 269, P. 106884 - 106884

Published: March 2, 2024

Language: Английский

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Microplastics aggravate the bioaccumulation and corresponding food safety risk of antibiotics in edible bivalves by constraining detoxification-related processes

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168436 - 168436

Published: Nov. 8, 2023

Language: Английский

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Tris(2-chloroethyl) Phosphate Leads to Unbalanced Circulating Erythrocyte in Mice by Activating both Medullary and Extramedullary Erythropoiesis

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Tris(2-chloroethyl) phosphate (TCEP), a prevalent organophosphorus flame retardant, has been identified in various environmental matrices and human blood samples, provoking alarm regarding its hematological toxicity, subject that not thoroughly investigated. Red cells (RBCs), or erythrocytes, are the predominant cell type peripheral crucial for maintenance of physiological health. This investigation employed oral gavage to examine effects TCEP exposure on erythrocyte counts mice clarify underlying mechanisms. The results demonstrated marked increase circulating RBC post-TCEP exposure, concomitantly heightening risk polycythemia vera (PV). stimulated erythropoiesis across all stages medullary development, including differentiation hematopoietic stem into erythroid progenitors, progression maturation erythrocyte. Moreover, potentiated extramedullary spleen liver. Subsequent bioinformatics analysis implied TCEP-induced was attributed p53 downregulation. Thus, these findings indicate disrupts erythrocyte-mediated homeostasis through enhancement both erythropoiesis, leading alteration equilibrium.

Language: Английский

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Degradation of organophosphate flame retardants by white-rot fungi: Degradation pathways and associated toxicity

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 959, P. 178260 - 178260

Published: Jan. 1, 2025

The environmental persistence of organophosphate flame retardants (OPFRs) in water is becoming and concern. White Rot Fungi (WRF) have proven its capability to degrade certain OPFRs such as tributyl phosphate (TBP), tris(2-butoxyethyl) (TBEP), tris(2-chloroethyl) (TCEP) tris(2-chloroisopropyl) (TCPP). Despite this capability, there limited knowledge about the specific pathways involved degradation. In study, three different WRF were paired with individual OPFRs, potential transformation products (TPs) identified by UHPLC-HRMS. Some compounds structures further validated NMR. From these data degradation proposed. TBP was degraded successive hydroxylation hydrolysis reactions, a novel dehydrogenation step suggested. Both TCEP TCPP underwent oxidative dechlorination, experiencing subsequent hydrolysis. Uncommon reductive dehalogenation also observed. environmentally relevant methylation. TBEP generated numerous TPs, mainly dealkylations, along hydroxylation. Notably, demethylation proposed for first time. Additional secondary formed through oxidation initial metabolites. Finally, vivo silico toxicity assessments conducted, identifying TPs potentially toxic.

Language: Английский

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Inhibition of platelet activation process upon tris (2-chloroethyl) phosphate exposure: Role of PFKP-mediated glycolysis and the pentose phosphate pathway

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125714 - 125714

Published: Jan. 1, 2025

Language: Английский

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Exposure to malathion impairs learning and memory of zebrafish by disrupting cholinergic signal transmission, undermining synaptic plasticity, and aggravating neuronal apoptosis

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137391 - 137391

Published: Jan. 1, 2025

Language: Английский

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