Abstract
Background
Phytophthora
root
rot
caused
by
the
oomycete
capsici
is
most
devastating
disease
in
pepper
production
worldwide,
and
current
management
strategies
have
not
been
effective
preventing
this
disease.
Therefore,
use
of
resistant
varieties
was
regarded
as
an
important
part
P.
.
However,
our
knowledge
molecular
mechanisms
underlying
defense
response
roots
to
P
infection
limited.
Methods
A
comprehensive
transcriptome
metabolome
approaches
were
used
dissect
genotype
A204
susceptible
A198
at
0,
24
48
hours
post-inoculation
(hpi).
Results
More
genes
metabolites
induced
hpi
than
A198,
suggesting
prompt
activation
responses
genotype,
which
can
attribute
two
proteases,
subtilisin-like
protease
xylem
cysteine
proteinase
1,
involved
pathogen
recognition
signal
transduction
A204.
Further
analysis
indicated
that
responded
with
fine
regulation
Ca
2+
-
salicylic
acid-mediated
signaling
pathways,
then
downstream
responses,
including
cell
wall
reinforcement
defense-related
expression
accumulation.
Among
them,
differentially
expressed
accumulated
flavonoid
biosynthesis
pathways
uniquely
activated
hpi,
indicating
a
significant
role
resistance
Conclusion
The
candidate
transcripts
may
provide
genetic
resources
be
useful
improvement
rot-resistant
characters
pepper.
In
addition,
model
proposed
study
provides
new
insight
into
against
pepper,
enhance
understanding
interaction
pepper–
Journal of Experimental Botany,
Год журнала:
2023,
Номер
74(8), С. 2726 - 2739
Опубликована: Фев. 1, 2023
Abstract
Cuticular
wax
protects
plants
from
various
biotic
and
abiotic
stresses.
However,
the
genetic
network
of
biosynthesis
environmental
factors
influencing
leaf
production
in
rapeseed
(Brassica
napus)
remains
unclear.
Here,
we
demonstrated
role
resistance
to
Sclerotinia
infection
rapeseed.
We
found
that
leaves
grown
under
high
light
intensity
had
higher
expression
genes
involved
biosynthesis,
produced
more
on
surface,
compared
with
those
low
conditions.
Genome-wide
association
study
(GWAS)
identified
89
single
nucleotide
polymorphisms
significantly
associated
coverage.
A
cross-analysis
between
GWAS
differentially
expressed
(DEGs)
epidermis
accessions
contrasting
differences
content
revealed
17
candidate
control
this
variation
Selective
sweep
analysis
combined
DEG
unveiled
510
significant
selective
signatures.
From
genes,
selected
BnaA02.LOX4,
a
putative
lipoxygenase,
BnaCnn.CER1,
BnaA02.CER3,
BnaC02.CER3,
BnaA01.CER4
(ECERIFERUM1–4)
were
putatively
responsible
for
analyse
allelic
forms
haplotypes
corresponding
or
These
data
enrich
our
knowledge
about
formation,
provide
gene
pool
breeding
an
ideal
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(21), С. 11928 - 11937
Опубликована: Май 16, 2024
The
discovery
of
structurally
distinct
leads
is
imperative
in
modern
agrochemical
science.
Inspired
by
eudistomins
Y
and
the
framework-related
pharmaceuticals,
aryl
heteroaryl
ketone
was
drawn
as
a
common
model
intriguing
design
divergent
synthesis
14
kinds
ketones
aligned
with
their
oxime
derivatives.
Antifungal
function-oriented
phenotypical
screen
protruded
benzothiazolyl-phenyl
5a
promising
model,
concomitant
modification
led
to
benzothiazolyl
5am
(EC50
=
5.17
μM)
superior
lead
than
fluoxastrobin
7.54
against
Sclerotinia
sclerotiorum.
Scaffold
hopping
phenyl
subunit
identified
benzothiazolyl-pyridyl
novel
antifungal
scaffold
accompanied
acquiring
5bm
remarkable
activity
3.57
Pyricularia
oryzae.
Molecular
docking
showed
that
candidate
could
form
more
hydrogen
bonds
amino
acid
residues
actin
metrafenone.
This
compound
also
demonstrated
better
curative
efficacy
metrafenone
controlling
plant
disease
caused
S.
These
results
rationalize
candidates
based
on
ketone.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
73(9), С. 5042 - 5053
Опубликована: Фев. 20, 2025
Oilseed
rape
is
one
of
the
most
important
oil
crops
worldwide.
Stem
rot
disease
rapeseed
caused
by
Sclerotinia
sclerotiorum,
posing
a
threat
to
crop
yield.
NTL
small
class
membrane-tethered
NAC
transcription
factors,
which
are
stored
on
membrane
in
dormant
form,
released
upon
stimuli,
and
then
transported
nucleus.
Here,
we
identified
BnaNTL1
from
oilseed
rape,
its
relocation
ER
nucleus
induced
S.
sclerotiorum.
Plants
overexpressing
BnaNTL1-ΔTM
(a
truncated
form
without
transmembrane
domain)
resistant
sclerotiorum
infection
accumulated
with
more
JA
ROS.
Genes
related
pathway
ROS
signal
were
significantly
BnaNTL1.
Furthermore,
dual-luciferase
EMSA
results
showed
that
directly
binds
promoter
regions
AOC3,
LOX2,
OPCL1,
PDF1.2,
it
activates
their
expression.
In
summary,
positively
regulates
plant
resistance
modulating
synthesis
production.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 10, 2025
Fenbuconazole,
a
chiral
triazole
fungicide,
is
produced
and
used
as
racemate.
Previous
toxicological
research
on
fenbuconazole
in
nontarget
organisms
primarily
the
racemate,
necessitating
an
investigation
into
each
enantiomer's
distribution
elimination
for
safety
assessment.
In
this
study,
absolute
configurations
of
enantiomers
were
first
confirmed
by
ECD,
designating
them
S-(+)-fenbuconazole
R-(-)-fenbuconazole
based
their
optical
activity.
The
UHPLC-QQQ/MS
method
was
selected
to
systematically
study
toxicokinetics
enantiomer
conversion
mice.
results
revealed
significant
enantioselectivity,
with
exhibiting
15.11
times
higher
AUC0-∞
than
R-(-)-fenbuconazole,
indicating
greater
blood
absorption.
experiment
involving
14
examined
tissues,
consistently
exceeded
levels,
except
stomach.
Notably,
concentration
liver
second
only
stomach
4.35
suggesting
propensity
hepatic
accumulation.
Molecular
docking
studies
further
demonstrated
stronger
interaction
between
CYP2B
enzyme
liver,
implying
hepatotoxic
potential.
Both
rarely
excreted
urine
or
feces,
cumulative
excretion
rate
below
2.5‰.
Enantiomer
occurred
unidirectionally
(R
→
S)
mice,
rates
generally
low
most
tissue.
Thus,
enantiomeric
not
primary
factor
driving
enantioselectivity.
summary,
exhibited
poor
absorption,
limited
distribution,
weak
enzyme,
which
may
be
considered
low-risk
product
that
could
guide
monomer
development
promote
reduction
pesticide
usage.
Plant Cell & Environment,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 17, 2025
ABSTRACT
Root
rot
in
Camellia
oleifera
complicates
the
development
of
targeted
control
measures
owing
to
its
complex
aetiology.
Although
breeding
resistant
varieties
C.
presents
a
promising
solution,
research
into
cultivation
strategies
and
potential
resistance
mechanisms
against
root
remains
limited.
In
this
study,
we
investigated
six
cultivars
that
exhibit
varying
levels
rot.
We
conducted
transcriptome
analysis,
measurements
soil
physicochemical
properties
an
analysis
fungal
microbiome
explore
relationship
between
Fusarium
‐induced
flavonoid
compounds
rhizosphere.
The
cultivar
CL18
demonstrated
superior
performance
concerning
incidence,
health
status
expression
genes
associated
with
biosynthesis
study.
Significant
differences
were
observed
composition
diversity
rhizosphere
communities
among
various
.
abundance
was
low,
negative
correlation
identified
content
Our
study
uncovers
role
flavonoids
rot,
thereby
offering
new
for
disease
management
cultivars.
PLoS Pathogens,
Год журнала:
2025,
Номер
21(5), С. e1013129 - e1013129
Опубликована: Май 2, 2025
Sclerotinia
stem
rot
is
a
devastating
disease
affecting
vegetables
and
oil
crops
worldwide.
It
caused
by
the
necrotrophic
ascomycete
(
S.
)
sclerotiorum.
Host-induced
gene
silencing
(HIGS)
has
shown
promise
in
control
against
insects
fungal
pathogens,
but
effective
HIGS
target
genes
sclerotiorum
remain
limited.
In
this
study,
we
identified
GDP-mannose
pyrophosphorylase
(GMPP)
SsMPG2
through
forward
genetic
analysis.
Ssmpg2
mutants
exhibit
abnormal
sclerotia
compound
appressoria,
along
with
defective
cell
wall
integrity
attenuated
virulence.
Meanwhile,
knocking
out
reduced
GMPP
activity
glycosylation
of
proteins.
addition,
interacts
SsMPG1,
which
essential
.
Downstream
SsMPG1-SsMPG2
complex,
SsPMT4
,
encodes
an
O-mannosyltransferase,
also
critical
for
appressoria
formation
Notably,
MPG2
virulence
several
other
pathogens
such
as
Botrytis
cinerea
Magnaporthe
oryzae
Fusarium
graminearum
Furthermore,
expressing
hairpin
RNAs
SsMPG1
Nicotiana
benthamiana
Arabidopsis
thaliana
significantly
symptoms
Collectively,
our
findings
demonstrate
roles
phytopathogenic
fungi
suggest
that
MPGs
are
promising
HlGS
targets
controlling
Biotechnology for Biofuels and Bioproducts,
Год журнала:
2022,
Номер
15(1)
Опубликована: Май 20, 2022
Abstract
Background
Rapeseed
(
Brassica
napus
)
is
the
second
largest
oil
crop
worldwide.
It
widely
used
in
food,
energy
production
and
chemical
industry,
as
well
being
an
ornamental.
Consequently,
it
has
a
large
economic
value
developmental
potential.
Waterlogging
important
abiotic
stress
that
restricts
plant
growth
development.
However,
little
known
about
molecular
mechanisms
underlying
waterlogging
tolerance
B.
napus.
Results
In
present
study,
physiological
changes
transcriptomes
of
germination-stage
rapeseed
response
to
were
investigated
cultivar
‘Zhongshuang
11’
(ZS11)
its
anthocyanin-more
am
mutant,
which
was
identified
our
previous
study.
The
mutant
showed
stronger
compared
with
ZS11,
significantly
increased
anthocyanin,
soluble
sugar
malondialdehyde
contents
decreased
chlorophyll
after
12
days
waterlogging.
An
RNA-seq
analysis
1370
2336
differently
expressed
genes
(DEGs)
responding
ZS11
,
respectively.
enrichment
revealed
DEGs
predominately
involved
carbohydrate
metabolism,
whereas
those
particularly
enriched
hormone
signal
transduction
endogenous
stimulation.
total,
299
anthocyanin
biosynthesis-related
structural
(24)
regulatory
encoding
transcription
factors
(275),
may
explain
content
mutant.
A
total
110
clustered
pathway
also
DEGs,
including
70
auxin
ethylene
changed
Furthermore,
expression
levels
16
putative
roles
accumulation
biotic/abiotic
responses
validated
by
quantitative
real-time
PCR
consistent
transcriptome
profiles.
Conclusion
This
study
provides
new
insights
into
stress,
should
be
useful
for
reducing
damage
caused
further
breeding
varieties
high
tolerance.
The Crop Journal,
Год журнала:
2024,
Номер
12(2), С. 493 - 502
Опубликована: Март 5, 2024
The
necrotrophic
fungus,
Sclerotinia
sclerotiorum,
employs
an
array
of
cell
wall-degrading
enzymes
(CWDEs),
including
cellulase,
to
dismantle
host
walls.
However,
the
molecular
mechanisms
through
which
S.
sclerotiorum
degrades
cellulose
remain
elusive.
Here,
we
unveil
a
novel
secretory
cellobiohydrolase,
SsdchA,
characterized
by
signal
peptide
and
Glyco_hydro_7
(GH7)
domain.
SsdchA
exhibits
robust
expression
during
early
infection
stages.
Interestingly,
colony
morphology
growth
rates
unaffected
across
wild-type,
deletion
strains
overexpression
on
potato
dextrose
agar
(PDA)
medium.
Nevertheless,
pathogenicity
cellobiohydrolase
activity
decreased
in
strains,
but
enhanced
strains.
Moreover,
heterologous
Arabidopsis
thaliana
leads
reduced
content
heightened
susceptibility
sclerotiorum.
Collectively,
our
data
underscore
pivotal
role
