Functional Annotation of Lactiplantibacillus plantarum 13-3 as a Potential Starter Probiotic Involved in the Food Safety of Fermented Products

Molecules, Год журнала: 2022, Номер 27(17), С. 5399 - 5399

Опубликована: Авг. 24, 2022

The important role of Lactiplantibacillus plantarum strains in improving the human mucosal and systemic immunity, preventing non-steroidal anti-provocative drug-induced reduction T-regulatory cells, as probiotic starter cultures food processing has motivated in-depth molecular genomic research these strains. current study, building on this concept, reveals importance 13-3 a potential bacteriocin-producing strain that helps condition digestive system thus enhances immunity living beings via various extracellular proteins exopolysaccharides. We have assessed stability quality L. genome through de novo assembly annotation FAST-QC RAST, respectively. probiotic-producing components, secondary metabolites, phage prediction sites, pathogenicity carbohydrate-producing enzymes also been analyzed computationally. This study is nonpathogenic with 218 subsystems 32,918 qualities five classes sugars several functions. Two hit sites identified strain. Cyclic lactone autoinducer, terpenes, T3PKS, RiPP-like gene clusters evidencing its processing. Combined, non-pathogenicity food-processing ability rendered industrially important. subsystem characterization provides starting point to investigate strain's healthcare-related applications well.

Язык: Английский

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Lactobacillus plantarum CCFM405 against Rotenone-Induced Parkinson’s Disease Mice via Regulating Gut Microbiota and Branched-Chain Amino Acids Biosynthesis

Nutrients, Год журнала: 2023, Номер 15(7), С. 1737 - 1737

Опубликована: Апрель 1, 2023

Recent studies have demonstrated that disturbances in the gut microbiota and -derived metabolites contribute to pathogenesis of Parkinson's disease (PD), suggesting probiotic treatments restore them may delay progression. This study aimed examine attenuating efficacy L. plantarum CCFM405 potential mechanisms mice with rotenone-induced PD. Our results indicate ameliorated motor deficits constipation, decreased dopaminergic neuronal death, reduced intestinal inflammation neuroinflammation, raised dopamine levels, 5-HT, associated striatal region brain Sequencing 16S rRNA from fecal revealed normalized bacterial composition PD, as evidenced by increased relative abundance following genus, Bifidobacterium, Turicibacter, Faecalibaculum, Alistipes, Bilophila, Akkermansia, Escherichia-Shigella. The PICRUSt-predicted function enhanced biosynthesis amino acid pathways, particularly valine, leucine, isoleucine (branched-chain acids, BCAAs). A non-metabolomic analysis serum feces showed markedly levels BCAAs. Pathway enrichment based on KEGG database further suggested supplementation can promote BCAAs biosynthesis. Collectively, help prevent PD modulating microbiota-metabolite axis. play a dominant role CCFM405-associated neuroprotection mice. could be utilized food supplement management

Язык: Английский

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Healthy Human Fecal Microbiota Transplantation into Mice Attenuates MPTP-Induced Neurotoxicity via AMPK/SOD2 Pathway

Aging and Disease, Год журнала: 2023, Номер 14(6), С. 2193 - 2193

Опубликована: Янв. 1, 2023

Increasing evidence has shown that gut dysbacteriosis may play a crucial role in neuroinflammation Parkinson's disease (PD). However, the specific mechanisms link microbiota to PD remain unexplored. Given critical roles of blood-brain barrier (BBB) dysfunction and mitochondrial development PD, we aimed evaluate interactions among microbiota, BBB, resistance oxidation inflammation PD. We investigated effects fecal transplantation (FMT) on physiopathology 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. The aim was explore from patients healthy human controls neuroinflammation, BBB components, antioxidative capacity via AMPK/SOD2 pathway. Compared control mice, MPTP-treated mice exhibited elevated levels Desulfovibrio, whereas given FMT enriched Akkermansia humans showed no significant alterations microbiota. Strikingly, significantly aggravated motor impairments, dopaminergic neurodegeneration, nigrostriatal glial activation colonic inflammation, inhibited signaling greatly improved aforementioned MPTP-caused effects. Surprisingly, displayed loss pericytes, which restored by controls. Our findings demonstrate can correct ameliorate neurodegeneration MPTP-induced mouse model suppressing microgliosis astrogliosis, ameliorating impairments pathway, restoring pericytes integrity. These raise possibility alteration be risk factor for provide potential application preclinical treatment.

Язык: Английский

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Integrated genome based evaluation of safety and probiotic characteristics of Lactiplantibacillus plantarum YW11 isolated from Tibetan kefir

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Апрель 20, 2023

The comparative genomic analysis of Lactiplantibacillus plantarum YW11 (L. YW11) isolated from Tibetan kefir involves comparison the complete genome sequences strain with other closely related L. strains. This type can be used to identify genetic diversity among strains and explore characteristics strain. was found composed a circular single chromosome 4,597,470 bp G + C content 43.2%. A total 4,278 open reading frames (ORFs) were identified in coding density 87.8%. conducted using two strains, C11 LMG21703. Genomic revealed that shared 72.7 75.2% gene LMG21703, respectively. Most genes between three involved carbohydrate metabolism, energy production conversion, amino acid transcription. In this analysis, 10 previously sequenced entire genomes species compared an in-silico technique discover divergence linked intake their potential adaptations distinct human intestinal environments. subspecies pan-genome open, which correlated its extraordinary capacity colonize several Phylogenetic novel homogenously grouped l Lactiplantibacillus. resistant cefoxitin, erythromycin, metronidazole, inhibited pathogens including Listeria monocytogenes, Clostridium difficile, Vibrio cholera, others, had excellent aerotolerance, is useful for industrial operations. provide insights into strain, further understand role kefir.

Язык: Английский

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The gut microbiota–brain axis in neurological disorders

MedComm, Год журнала: 2024, Номер 5(8)

Опубликована: Июль 20, 2024

Abstract Previous studies have shown a bidirectional communication between human gut microbiota and the brain, known as microbiota–gut–brain axis (MGBA). The MGBA influences host's nervous system development, emotional regulation, cognitive function through neurotransmitters, immune modulation, metabolic pathways. Factors like diet, lifestyle, genetics, environment shape composition together. Most research explored how regulates host physiology its potential in preventing treating neurological disorders. However, individual heterogeneity of microbiota, strains playing dominant role diseases, interactions these microbial metabolites with central/peripheral systems still need exploration. This review summarizes driving neurodevelopmental disorders (autism spectrum disorder attention deficit/hyperactivity disorder), neurodegenerative diseases (Alzheimer's Parkinson's disease), mood (anxiety depression) recent years discusses current clinical preclinical microbe‐based interventions, including dietary intervention, probiotics, prebiotics, fecal transplantation. It also puts forward insufficient on provides framework for further

Язык: Английский

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Understanding Ligilactobacillus salivarius from Probiotic Properties to Omics Technology: A Review

Foods, Год журнала: 2024, Номер 13(6), С. 895 - 895

Опубликована: Март 15, 2024

Ligilactobacillus salivarius (basonym: Lactobacillus salivarius, L. salivarius) is a type of lactic acid bacteria (LAB) commonly found in the oropharyngeal-gastrointestinal tract (OGT). It has gained significant attention due to its probiotic and functional properties as well various health-promoting roles. strains exhibit strong resistance adhesion OGT along with outstanding antioxidant antimicrobial properties. Additionally, numerous have ability produce bacteriocins antagonistic activity. These characteristics indicate remarkable potential promoting favorable effects on human health. also been observed that positive effect composition intestinal microbiota, thereby improving metabolic profiling healthy balanced internal environment. In recent years, multi-omics technologies such genomics, transcriptomics, proteomics metabolomics employed gain deeper understanding roles mechanisms associated This review aims provide an overview containing specific interactions host microflora, insights from omics studies.

Язык: Английский

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Microbiome in radiotherapy: an emerging approach to enhance treatment efficacy and reduce tissue injury

Molecular Medicine, Год журнала: 2024, Номер 30(1)

Опубликована: Июль 19, 2024

Abstract Radiotherapy is a widely used cancer treatment that utilizes powerful radiation to destroy cells and shrink tumors. While can be beneficial, it also harm the healthy tissues surrounding tumor. Recent research indicates microbiota, collection of microorganisms in our body, may play role influencing effectiveness side effects therapy. Studies have shown specific species bacteria living stomach influence immune system’s response radiation, potentially increasing treatment. Additionally, microbiota contribute adverse like radiation-induced diarrhea. A potential strategy enhance radiotherapy outcomes capitalize on microbiome involves using probiotics. Probiotics are offer health benefits when consumed sufficient quantities. Several studies indicated probiotics alter composition gut resulting an enhanced therapy consequently improving efficacy It important note disrupt natural balance bacteria, increased intestinal permeability inflammatory conditions. These disruptions lead such as diarrhea damage lining. The emerging field offers promising avenue for optimizing outcomes. This paper aims provide overview human its augmenting while minimizing damage.

Язык: Английский

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Biotransformation characteristics of urate-lowering probiotic fermented apple juice and potential regulatory mechanisms for ameliorating hyperuricemia via mediating gut microbiota and metabolic pathways

Food Chemistry, Год журнала: 2024, Номер 460, С. 140462 - 140462

Опубликована: Июль 17, 2024

Язык: Английский

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Gut Microbiota-Based Interventions for Parkinson’s Disease: Neuroprotective Mechanisms and Current Perspective

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Язык: Английский

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Study insights in the role of PGC-1α in neurological diseases: mechanisms and therapeutic potential

Frontiers in Aging Neuroscience, Год журнала: 2025, Номер 16

Опубликована: Фев. 12, 2025

Peroxisome proliferator-activated receptor- γ coactivator-1α (PGC-1α), which is highly expressed in the central nervous system, known to be involved regulation of mitochondrial biosynthesis, metabolic regulation, neuroinflammation, autophagy, and oxidative stress. This knowledge indicates a potential role PGC-1α wide range functions associated with neurological diseases. There emerging evidence indicating protective pathogenesis several As such, deeper broader understanding its diseases urgently needed. The present review provides relatively complete overview current on PGC-1α, including different types neurons, basic structural characteristics, interacting transcription factors. Furthermore, we various diseases, such as intracerebral hemorrhage, ischemic stroke, Alzheimer’s disease, Parkinson’s Amyotrophic lateral sclerosis, Huntington’s other PolyQ Importantly, discuss some compounds or drug-targeting strategies that have been studied ameliorate pathology these introduce possible mechanistic pathways. Based available studies, propose targeting could serve promising novel therapeutic strategy for one more

Язык: Английский

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