Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella DOI
Yuxi Liu, Chao Hu, Yuting Li

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 72(1), С. 363 - 377

Опубликована: Дек. 22, 2023

While previous studies have reported G protein-coupled receptor (GPCR)-mediated insecticide resistance in various arthropods, the understanding of GPCR-associated mechanisms Cydia pomonella remains limited. In this study, a total 95 CpGPCR genes categorized into four families were identified C. pomonella. Results revealed high expression levels majority CpGPCRs during first larval stage and head Exposure to lambda-cyhalothrin significantly increased 15 CpGPCRs, including CpGPCR70, which is highly expressed all stages shows highest midgut. RNA interference (RNAi) demonstrated that downregulation CpGPCR70 leads reduced key resistance-related decreased tolerance larvae lambda-cyhalothrin. These findings indicate plays crucial role regulating detoxifying involved resistance, offering valuable insights for development more effective pest control strategies.

Язык: Английский

Transcriptional regulation and overexpression of GST cluster enhances pesticide resistance in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) DOI Creative Commons
Minghui Jin, Peng Yan, Jie Peng

и другие.

Communications Biology, Год журнала: 2023, Номер 6(1)

Опубликована: Окт. 19, 2023

The rapid evolution of resistance in agricultural pest poses a serious threat to global food security. However, the mechanisms through metabolic regulation are largely unknown. Here, we found that GST gene cluster was strongly selected North China (NTC) population, and it significantly genetically-linked lambda-cyhalothrin resistance. Knockout using CRISPR/Cas9 increased sensitivity knockout strain lambda-cyhalothrin. Haplotype analysis revealed no non-synonymous mutations or structural variations cluster, whereas GST_119 GST_121 were overexpressed NTC population. Silencing co-silencing with RNAi larval We also identified additional GATAe transcription factor binding sites promoter NTC_GST_119. Transient expression Hi5 cells activated NTC_GST_119 Xinjiang (XJ)_GST_119 transcription, but transcriptional activity higher than XJ_GST_119. These results demonstrate regulatory region result complex changes which enhances field-populations. This study deepens our knowledge evolutionary mechanism adaptation under environmental stress provides potential targets for monitoring integrated management.

Язык: Английский

Процитировано

26
The exposure risk of heavy metals to insect pests and their impact on pests occurrence and cross-tolerance to insecticides: A review DOI
Shanchun Yan,

Mingtao Tan,

Aoying Zhang

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 916, С. 170274 - 170274

Опубликована: Янв. 21, 2024

Язык: Английский

Процитировано

13
Secondary metabolites in host pears defense against two fruit borers and cytochrome-P450-mediated counter-defense DOI Creative Commons

Shi-Pan Zhang,

Bing Bai,

Gao-Man Chen

и другие.

iScience, Год журнала: 2024, Номер 27(4), С. 109518 - 109518

Опубликована: Март 16, 2024

Язык: Английский

Процитировано

10
Two Point Mutations in CYP4CE1 Promoter Contributed to the Differential Regulation of CYP4CE1 Expression by FoxO between Susceptible and Nitenpyram-Resistant Nilaparvata lugens DOI
Huihui Zhang, Xumin Lin,

Baojun Yang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(3), С. 1779 - 1786

Опубликована: Янв. 12, 2024

Four P450s were reported to be important for imidacloprid resistance in Nilaparvata lugens, a major insect pest on rice, which was confirmed this study an imidacloprid-resistant strain (ImiR). Here we found that only two (CYP4CE1 and CYP6ER1) from these four P450 genes overexpressed nitenpyram-resistant (NitR) when compared susceptible (SUS). CYP4CE1 RNAi reduced nitenpyram NitR ImiR strains, with greater reduction resistance. The transcription factor FoxO mediated but it not differentially expressed among strains. potential reason the differential regulation of expression mainly sequence differences promoter between resistant insects. In six response elements predicted promoter, single-nucleotide polymorphisms frequently detected over 50% individuals. luciferase reporter assays showed mutations, −650T/G −2205T/A at positions −648 −2200 bp, contributed enhanced by frequency 69% both individuals less than 20% SUS conclusion, overexpression importantly N. mutations insects led FoxO.

Язык: Английский

Процитировано

9
The Synergistic Effect of Lemongrass Essential Oil and Flometoquin, Flonicamid, and Sulfoxaflor on Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae): Insights into Toxicity, Biochemical Impact, and Molecular Docking DOI Creative Commons
Moataz A. M. Moustafa, Fatma S. Ahmed,

Nawal Abdulaziz Alfuhaid

и другие.

Insects, Год журнала: 2024, Номер 15(5), С. 302 - 302

Опубликована: Апрель 24, 2024

The whitefly, Bemisia tabaci (Genn.), is one of the most dangerous polyphagous pests in world. Eco-friendly compounds and new chemical insecticides have gained recognition for whitefly control. In this study, toxicity biochemical impact flometoquin, flonicamid, sulfoxaflor, alone or combined with lemongrass essential oil (EO), against B. was studied. addition, a molecular docking study conducted to assess binding affinity tested AchE. Based on LC values, descending order adults as follows: sulfoxaflor > flonicamid flometoquin EO. binary mixtures each EO exhibited synergism all combinations, observed mortalities ranging from 15.09 22.94% higher than expected an additive effect. Sulfoxaflor combination EO, significantly inhibited AchE activity while only demonstrated significant α-esterase, none affected cytochrome P450 GST. However, specific by lemongrass/sulfoxaflor mixture α-esterase lemongrass/flometoquin mixture. Moreover, energy scores −4.69 −7.06 kcal/mol. current findings provide foundation utilizing combinations oils integrated pest management (IPM) tabaci.

Язык: Английский

Процитировано

8
Responses of biological characteristics and detoxification enzymes in the fall armyworm to methoxyfenozide stress DOI
Shuqi Yang, Yue Yuan,

Xiongwei Zhang

и другие.

Journal of Economic Entomology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 20, 2025

Abstract Methoxyfenozide is an insecticide with a unique mode of action on the insect ecdysone receptor and has been registered for control pests all over world. In present work, Spodoptera frugiperda was exposed to sublethal lethal concentrations methoxyfenozide determine its impact specific biological traits, metabolic enzyme activity, expression detoxification enzymes. The result showed that 72-h posttreatment LC50 LC70 significantly reduced fecundity (eggs/female) F0 generation compared those group. However, duration prepupal period increased. exposure LC10, LC30, LC50, extended developmental larvae in F1 individuals. decreased, population life table parameters were also affected. activity carboxylesterases little significant change, whereas glutathione S-transferases (GSTs) cytochrome P450 monooxygenases (P450s) altered after methoxyfenozide. total, 24-, 48-, 96-h could cause upregulation genes such as CYP6AE44, CYP6B39, CYP9A26, CYP9A58, CYP9A59, CYP9A60, well GST including GSTe3, GSTe9, GSTe10, GSTe15, GSTo2, GSTs1, GSTs5, GSTm2, GSTm3. These findings be instrumental elucidating molecular mechanisms underlying effects S. frugiperda.

Язык: Английский

Процитировано

1
Functional analysis of the epsilon glutathione S-transferases in the adaptation of Spodoptera litura to xanthotoxin DOI

Tianxiang Xiao,

Mengqing Deng,

Xiaodan Huang

и другие.

Insect Biochemistry and Molecular Biology, Год журнала: 2025, Номер 180, С. 104299 - 104299

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

1
Biological and biochemical responses of Panonychus citri (Acari: Tetranychidae) exposed to sublethal concentrations of cyflumetofen DOI
Tianrong Xin,

Xinyan Kong,

Chenyu Jiang

и другие.

Crop Protection, Год журнала: 2024, Номер 178, С. 106604 - 106604

Опубликована: Янв. 24, 2024

Язык: Английский

Процитировано

6
miR-317-3p and miR-283-5p Play a Crucial Role in Regulating the Resistance to Indoxacarb in Spodoptera frugiperda by Targeting GSTs4 DOI

Mengyan Wu,

Haixiang Lv,

Zhimin Guo

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(13), С. 6889 - 6899

Опубликована: Март 21, 2024

Spodoptera frugiperda is primarily controlled through chemical insecticides. Our RNA-seq data highlight the overexpression of GSTs4 in indoxacarb-resistant S. frugiperda. However, exact role indoxacarb resistance and its regulatory mechanisms remains elusive. Therefore, we investigated functional explored underlying post-transcriptional mechanisms. was highly overexpressed (27.6-fold) strain, silencing significantly increases susceptibility to indoxacarb, increasing mortality by 27.3%. miR-317-3p miR-283-5p can bind 3′UTR GSTs4, targeting relationship confirmed dual-luciferase reporter assays. Injecting agomirs reduces levels 64.8 42.3%, respectively, resulting an increased indoxacarb. Conversely, administration miR-283-5pantagomirs expression larval These findings demonstrate that contribute regulating GSTs4.

Язык: Английский

Процитировано

5
Chlorantraniliprole Resistance in Spodoptera frugiperda: Resistance Monitoring, Resistance Risk, and Resistance Mechanisms DOI

Zhimin Guo,

Huina Ma,

Jiahui Tang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(30), С. 16651 - 16660

Опубликована: Июль 22, 2024

is a significant global pest, and chlorantraniliprole (CAP) extensively used in China for its control. Understanding CAP resistance

Язык: Английский

Процитировано

5