Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 15, 2024
Introduction
Extracellular
vesicles
(EVs)
facilitate
inter
and
intra-species/kingdom
communication
through
biomolecule
transfer,
including
proteins
small
RNAs.
Plant-derived
EVs,
a
hot
topic
in
the
field,
hold
immense
capability
both
as
potential
biomarker
to
study
plant
physiology
biomaterial
that
can
be
mass-produced
used
various
industries
ranging
from
cosmetics
food
additives
biological
pesticides.
However,
systematic
characterization
of
EVs
is
required
establish
foundation
for
further
applications
studies.
Methods
In
this
study,
were
enriched
hydroponically
cultivated
potato
plants
(
Solanum
tuberosum
,
cv.
Laura).
We
isolated
root
exudates
apoplastic
wash
peels
using
vacuum
infiltration.
These
then
systematically
characterized
their
biophysical
chemical
properties
compare
with
standard
EV
characteristics
explore
roles
physiology.
Results
Biophysical
analyses
revealed
morphological
similarities
between
peel-derived
EVs.
The
average
diameter
root-derived
(164.6
±
7.3
nm)
was
significantly
larger
than
(132.2
2.0
nm,
p
<
0.004).
Liquid
chromatography–mass
spectrometry
(LC-MS)
demonstrated
substantial
protein
enrichment
purified
compared
crude
samples,
42%
25%
peel
Only
11.8%
identified
common
just
2%
shared.
Enriched
pathways
proteomes
associated
responses
biotic
abiotic
stress,
suggesting
defensive
role
plants.
Discussion
With
experimentation
elucidate
specific
methods
communication,
these
findings
increase
details
known
about
terms
physical
functions,
aiding
sustainable
agricultural
waste
utilization
large-scale
production,
aligning
concept
“valorization”.
Aim
This
study
aims
to
explore
a
sustainable
and
scalable
approach
using
tomato
fruit-derived
sEVs
(TsEVs)
deliver
calcitriol
for
enhanced
anticancer
effects,
addressing
challenges
of
low
yield
high
costs
associated
with
mammalian
cell-derived
sEVs.
Foods,
Год журнала:
2024,
Номер
13(10), С. 1492 - 1492
Опубликована: Май 11, 2024
Agri-food
wastes
generated
by
industrial
food
processing
are
valorized
through
the
extraction
of
biomolecules
to
obtain
value-added
products
useful
for
various
applications.
In
present
review,
we
describe
valuable
by-products
and
bioactive
molecules
that
can
be
obtained
from
agricultural
propose
extracellular
vesicles
(EVs)
as
innovative
nutraceutical
therapeutic
compounds
could
derived
agriculture
residues.
To
support
this
idea,
described
general
features
roles
EVs
focused
on
plant-derived
(PDEVs)
considered
natural
carriers
involved
in
intercellular
communication
between
diverse
kingdoms
life.
Consistently,
PDEVs
exert
beneficial
effects
(anti-inflammatory,
anti-tumor,
immune-modulatory)
mammalian
cells.
Although
research
field
is
currently
its
infancy,
near
future,
isolation
their
use
tools
represent
a
new
way
valorize
waste
agri-food
industry
an
ecofriendly
way.
Processes,
Год журнала:
2024,
Номер
12(12), С. 2938 - 2938
Опубликована: Дек. 23, 2024
Plant-derived
extracellular
vesicles
(PDEVs)
are
lipid
bilayer
nanoparticles,
naturally
produced
by
plant
cells,
with
sizes
ranging
from
50
to
500
nm.
Recent
studies
have
highlighted
their
great
potential
in
the
biotechnological
and
medical
fields,
due
natural
origin,
high
biocompatibility
intrinsic
therapeutic
properties.
PDEVs
contain
a
complex
biological
cargo
of
proteins,
lipids,
nucleic
acids
secondary
metabolites,
including
antioxidants
anti-inflammatory
molecules,
making
them
ideal
for
biomedical
applications
such
as
drug
delivery.
These
play
key
role
intercellular
communication
gene
regulation,
proving
be
particularly
promising
personalized
medicine.
ability
improve
stability
bioavailability,
optimizing
targeted
release
minimizing
side
effects.
Despite
some
challenges,
compositional
variability
need
standardized
protocols,
at
gunsight
innovative
research
aimed
improving
loading
capacity
specificity.
This
review
aims
provide
comprehensive
overview
PDEVs,
exploring
structure,
isolation
methods,
functional
characteristics,
applications,
highlighting
advantages
over
synthetic
nanoparticles
animal-derived
vesicles,
leading
an
sustainable
solution
development
new
approaches.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Март 10, 2025
Metabolic-Associated
Fatty
Liver
Disease
(MAFLD)
is
the
most
common
chronic
liver
disease
worldwide,
associated
with
systemic
metabolic
dysregulation.
It
can
progress
from
simple
hepatic
steatosis
(MAFL)
to
more
severe
conditions
like
Steatohepatitis
(MASH),
fibrosis,
cirrhosis,
and
Hepatocellular
Carcinoma
(HCC).
There
a
critical
lack
of
reliable
non-invasive
diagnostic
methods
effective
pharmaceutical
treatments
for
MAFLD/MASH,
emphasizing
need
further
research.
Extracellular
vesicles
(EVs)
are
nanoscale
structures
that
play
important
roles
in
cell
signaling
by
delivering
bioactive
molecules.
However,
there
significant
gap
literature
regarding
EVs
hosts,
plants,
microbiota
MAFLD.
This
review
explores
potential
various
sources—host,
microbiota—as
biomarkers,
therapeutic
agents,
drug
carriers,
treatment
targets
Firstly,
host-derived
extracellular
MAFLD,
focus
on
cell-type
specific
their
components—proteins,
miRNAs,
lipids—for
diagnosis
monitoring
were
discussed.
Moreover,
it
highlighted
mesenchymal
stem
(MSC)-derived
reducing
lipid
accumulation
injury,
immune
cell-derived
mitigating
inflammation
fibrosis.
The
also
discussed
use
as
carriers
due
ability
deliver
molecules
impact
mechanisms.
Additionally,
summarized
research
plant-derived
EVs,
which
help
reduce
accumulation,
inflammation,
enhance
gut
barrier
function
Also,
explored
microbial-derived
novel
targets,
particularly
relation
insulin
resistance,
dysfunction
Overall,
exploring
diverse
host,
plant,
sources
this
offers
valuable
insights
into
biomarkers
strategies,
could
pave
way
options
increasingly
prevalent
disease.
Notably,
challenges
translating
clinical
practice
thoroughly
discussed,
aiming
provide
possible
directions
strategies
future
Toxics,
Год журнала:
2024,
Номер
12(9), С. 653 - 653
Опубликована: Сен. 5, 2024
Polycyclic
aromatic
hydrocarbons
(PAHs)
are
persistent
organic
pollutants
that
pose
significant
environmental
and
health
risks.
These
compounds
originate
from
both
natural
phenomena,
such
as
volcanic
activity
wildfires,
anthropogenic
sources,
including
vehicular
emissions,
industrial
processes,
fossil
fuel
combustion.
Their
classification
carcinogenic,
mutagenic,
teratogenic
substances
link
them
to
various
cancers
disorders.
PAHs
categorized
into
low-molecular-weight
(LMW)
high-molecular-weight
(HMW)
groups,
with
HMW
exhibiting
greater
resistance
degradation
a
tendency
accumulate
in
sediments
biological
tissues.
Soil
serves
primary
reservoir
for
PAHs,
particularly
areas
of
high
creating
substantial
risks
through
ingestion,
dermal
contact,
inhalation.
Coastal
aquatic
ecosystems
especially
vulnerable
due
concentrated
human
activities,
PAH
persistence
disrupting
microbial
communities,
inhibiting
plant
growth,
altering
ecosystem
functions,
potentially
leading
biodiversity
loss.
In
plants,
contamination
manifests
form
abiotic
stress,
inducing
oxidative
cellular
damage,
growth
inhibition.
Plants
respond
by
activating
antioxidant
defenses
stress-related
pathways.
A
notable
aspect
defense
mechanisms
involves
plant-derived
extracellular
vesicles
(PDEVs),
which
membrane-bound
nanoparticles
released
cells.
PDEVs
play
crucial
role
enhancing
facilitating
intercellular
communication
coordinating
responses.
The
interaction
between
PDEVs,
while
not
fully
elucidated,
suggests
complex
interplay
mechanisms.
may
contribute
detoxification
pollutant
sequestration
or
delivering
enzymes
capable
degradation.
Studying
provides
valuable
insights
stress
resilience
offers
potential
new
strategies
mitigating
PAH-induced
plants
ecosystems.
Plant-derived
extracellular
vesicles
(PDEVs)
are
nanoscale
released
from
plant
cells
into
the
space.
While
similar
in
structure
and
function
to
mammalian-derived
EVs,
PDEVs
unique
due
their
origin
specific
metabolites
they
carry.
have
gained
significant
attention
recent
years,
with
numerous
reports
isolating
different
various
plants,
each
exhibiting
diverse
biological
functions.
However,
field
is
still
its
early
stages,
many
issues
need
further
exploration.
To
better
develop
utilize
PDEVs,
it
essential
a
comprehensive
understanding
of
characteristics.
This
review
provides
an
overview
advances
PDEV
research.
It
focuses
on
methods
techniques
for
purifying
comparing
respective
advantages,
limitations,
application
scenarios.
Furthermore,
we
discuss
latest
discoveries
regarding
composition
including
lipids,
proteins,
nucleic
acids,
metabolites.
Additionally,
detail
advanced
studies
multiple
functions
PDEVs.
Our
goal
advance
our
encourage
exploration
PDEV-based
science
technology,
offering
insights
potential
applications
human
health.
