Опубликована: Авг. 26, 2023
A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward in biological analysis using inverted microscope. The signal-to-noise ratio improved by a factor ca. 3 compared conventional CCD mode through use EM gain. When cultured epithelial cells configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified exposure time only 100 ms/pixel.
Язык: Английский
Journal of Raman Spectroscopy, Год журнала: 2025, Номер unknown
Опубликована: Март 22, 2025
ABSTRACT Bacterial discrimination using single‐cell Raman spectroscopy and machine/deep learning techniques has been widely explored for promising applications in medical, environmental, food sciences. To construct a machine‐learning model that can achieve highly accurate robust of bacteria real‐world samples, data consisting spectra bacterial cells acquired under various physiological conditions are essential. Despite much effort to study the effects growth phase on discrimination, it is not yet fully elucidated which phase(s) needs be included training efficiently improve accuracy what phase‐dependent changes cellular components underlie discrimination. Here, we used random forest (RF), an ensemble machine method, discriminate six species, including both Gram‐positive Gram‐negative bacteria, at five different phases ranging from lag late stationary phases. We compared four RF classification models were trained one (either midexponential or stationary), two (midexponential all The species built distinctly exceeded 80% with marked increase 24% 32.5% relative single phase. This was greater than found going (13%). also revealed bands relatively invariant (e.g., proteins) specific DNA/RNA intracellular storage materials) important attaining present provides simple effective way good performance, could extended other such as nutrient, temperature, pH.
Язык: Английский
Процитировано
0
Bioorganic & Medicinal Chemistry, Год журнала: 2025, Номер 126, С. 118222 - 118222
Опубликована: Май 2, 2025
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Ноя. 11, 2024
Abstract Cellular senescence occurs through the accumulation of many kinds stresses. Senescent cells in tissues also cause various age-related disorders. Therefore, detecting them without labeling is beneficial for medical research and developing diagnostic methods. However, existing biomarkers have limitations requiring fixation labeling, or their molecular backgrounds are uncertain. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging a novel option because it can assess visualize structures based on fingerprint. Here, we present new label-free method to cellular using CARS nucleoli. We found peak nucleolar amide I band shifted higher wavenumber binuclear senescent cells, which reflects changes protein secondary structure from predominant α-helices β-sheets originating amyloid-like aggregates. Following this, developed procedure that by providing ratios subtractions these two components. confirmed aggregates due unfolded/misfolded proteins produced proteasome inhibition. Finally, this help defects naïve even before binucleation. Thus, our evaluate ongoing defects.
Язык: Английский
Процитировано
2
Optics Continuum, Год журнала: 2023, Номер 2(9), С. 2044 - 2044
Опубликована: Сен. 5, 2023
A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward in biological analysis using inverted microscope. The signal-to-noise ratio improved by a factor ca. 3 compared conventional CCD mode through use EM gain. When epithelial cells configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified exposure time only 100 ms/pixel.
Язык: Английский
Процитировано
3
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 3, 2024
Abstract Cellular senescence occurs through the accumulation of many kinds stresses. Senescent cells in tissues also cause various age-related disorders. Therefore, detecting them without labeling is beneficial. However, existing biomarkers have limitations requiring fixation and labeling, or their molecular backgrounds are uncertain. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging a novel option because it can assess visualize structures based on fingerprint. Here, we present new label-free method to cellular by obtaining fingerprint signals nucleoli using CARS microspectroscopic system. We found peak nucleolar amide I band shifted higher wavenumber binuclear senescent cells, which reflects changes protein secondary structure from predominant α-helices β-sheets originating amyloid-like aggregates. Following this, developed procedure that providing ratios subtractions these two components. confirmed aggregates due unfolded/misfolded proteins produced proteasome inhibition. Finally, this help defects naïve even before binucleation. Thus, our beneficial evaluate ongoing defects.
Язык: Английский
Процитировано
0
Molecules, Год журнала: 2024, Номер 29(14), С. 3307 - 3307
Опубликована: Июль 13, 2024
Nanoparticles have been widely applied to treat emulsion-containing wastewater in the form of chemical demulsifiers, such as SiO
Язык: Английский
Процитировано
0
Applied Physics Express, Год журнала: 2024, Номер 17(9), С. 092001 - 092001
Опубликована: Июль 16, 2024
Abstract We analyzed the effects of a hypo-osmotic environment on rat Schwann cells, type glial cell surrounding neurons, using ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) microscopy. After treatment, we detected vacuole-like components in cytoplasm both bright-field and CARS spectroscopic imaging. An approach integrating morphological examination analysis based multiple vibrational bands revealed that these structures are predominantly water-filled, their characteristics closely resembled those vacuoles observed plant cells.
Язык: Английский
Процитировано
0
Chemical & Biomedical Imaging, Год журнала: 2024, Номер 2(10), С. 689 - 697
Опубликована: Июль 25, 2024
The Raman fingerprint spectral region provides abundant structural information on molecules. However, analyzing vibrational images within this using coherent imaging remains challenging due to the small cross section and congested features. In study, we combined ultrabroadband anti-Stokes scattering (CARS) microspectroscopy across range of 500-4000 cm
Язык: Английский
Процитировано
0
Journal of Molecular Liquids, Год журнала: 2024, Номер unknown, С. 125977 - 125977
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2024, Номер 96(46), С. 18322 - 18336
Опубликована: Окт. 22, 2024
Fourier transform coherent anti-Stokes Raman scattering (FT-CARS) spectroscopy is a powerful spectroscopic method that combines the principles of with (CARS). This stands out in for its ability to rapidly acquire spectra, achieving an impressive rate over 10 000 spectra per second. The involves scanning optical delay between two femtosecond pulses; initial pulse induces vibrational coherence sample, while subsequent probes this increasing delays. intensity generated modulated by dynamics enabling retrieval through transformation. Over past decades, FT-CARS has undergone substantial evolution, paving way application wide array fields, including material analysis and flow cytometry. In comprehensive Review, we explore fundamental diverse applications delve into potential future advances challenges associated emerging method.
Язык: Английский
Процитировано
0