Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera DOI Open Access
Yusuke Murakami,

Minami Yoshimura,

W. J. Niels Klement

et al.

Published: Aug. 26, 2023

A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward in biological analysis using inverted microscope. The signal-to-noise ratio improved by a factor ca. 3 compared conventional CCD mode through use EM gain. When cultured epithelial cells configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified exposure time only 100 ms/pixel.

Language: Английский

Improving Machine Learning–Based Bacterial Discrimination by Learning Single‐Cell Raman Data From Multiple Growth Phases DOI Open Access

Norihiko Oda,

Nanako Kanno, Shingo Kato

et al.

Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

ABSTRACT Bacterial discrimination using single‐cell Raman spectroscopy and machine/deep learning techniques has been widely explored for promising applications in medical, environmental, food sciences. To construct a machine‐learning model that can achieve highly accurate robust of bacteria real‐world samples, data consisting spectra bacterial cells acquired under various physiological conditions are essential. Despite much effort to study the effects growth phase on discrimination, it is not yet fully elucidated which phase(s) needs be included training efficiently improve accuracy what phase‐dependent changes cellular components underlie discrimination. Here, we used random forest (RF), an ensemble machine method, discriminate six species, including both Gram‐positive Gram‐negative bacteria, at five different phases ranging from lag late stationary phases. We compared four RF classification models were trained one (either midexponential or stationary), two (midexponential all The species built distinctly exceeded 80% with marked increase 24% 32.5% relative single phase. This was greater than found going (13%). also revealed bands relatively invariant (e.g., proteins) specific DNA/RNA intracellular storage materials) important attaining present provides simple effective way good performance, could extended other such as nutrient, temperature, pH.

Language: Английский

Citations

0
Apoptosis induction by ceramide derivatives and its potential mechanisms through domain formation DOI Creative Commons

Koya Tsujimura,

Miho Yakabe,

Hideaki Kano

et al.

Bioorganic & Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 126, P. 118222 - 118222

Published: May 2, 2025

Language: Английский

Citations

0
Coherent Raman microscopy visualizes ongoing cellular senescence through amide I peak shifts originating from β sheets in disordered nucleolar proteins DOI Creative Commons
S Ishibashi, Akihito Inoko, Yuki Oka

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 11, 2024

Abstract Cellular senescence occurs through the accumulation of many kinds stresses. Senescent cells in tissues also cause various age-related disorders. Therefore, detecting them without labeling is beneficial for medical research and developing diagnostic methods. However, existing biomarkers have limitations requiring fixation labeling, or their molecular backgrounds are uncertain. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging a novel option because it can assess visualize structures based on fingerprint. Here, we present new label-free method to cellular using CARS nucleoli. We found peak nucleolar amide I band shifted higher wavenumber binuclear senescent cells, which reflects changes protein secondary structure from predominant α-helices β-sheets originating amyloid-like aggregates. Following this, developed procedure that by providing ratios subtractions these two components. confirmed aggregates due unfolded/misfolded proteins produced proteasome inhibition. Finally, this help defects naïve even before binucleation. Thus, our evaluate ongoing defects.

Language: Английский

Citations

2
Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera DOI Creative Commons
Yusuke Murakami,

Minami Yoshimura,

W. J. Niels Klement

et al.

Optics Continuum, Journal Year: 2023, Volume and Issue: 2(9), P. 2044 - 2044

Published: Sept. 5, 2023

A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward in biological analysis using inverted microscope. The signal-to-noise ratio improved by a factor ca. 3 compared conventional CCD mode through use EM gain. When epithelial cells configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified exposure time only 100 ms/pixel.

Language: Английский

Citations

3
Coherent Raman microscopy detects nucleolar defects through amide I peak shifts originating from b-sheets: an application to visualizing ongoing cellular senescence DOI
S Ishibashi, Akihito Inoko, Yuki Oka

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Abstract Cellular senescence occurs through the accumulation of many kinds stresses. Senescent cells in tissues also cause various age-related disorders. Therefore, detecting them without labeling is beneficial. However, existing biomarkers have limitations requiring fixation and labeling, or their molecular backgrounds are uncertain. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging a novel option because it can assess visualize structures based on fingerprint. Here, we present new label-free method to cellular by obtaining fingerprint signals nucleoli using CARS microspectroscopic system. We found peak nucleolar amide I band shifted higher wavenumber binuclear senescent cells, which reflects changes protein secondary structure from predominant α-helices β-sheets originating amyloid-like aggregates. Following this, developed procedure that providing ratios subtractions these two components. confirmed aggregates due unfolded/misfolded proteins produced proteasome inhibition. Finally, this help defects naïve even before binucleation. Thus, our beneficial evaluate ongoing defects.

Language: Английский

Citations

0
Facile Synthesis of Novel Magnetic Janus Graphene Oxide for Efficient and Recyclable Demulsification of Crude Oil-in-Water Emulsion DOI Creative Commons

Yingbiao Xu,

Li Cheng, Wang Ye

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(14), P. 3307 - 3307

Published: July 13, 2024

Nanoparticles have been widely applied to treat emulsion-containing wastewater in the form of chemical demulsifiers, such as SiO

Language: Английский

Citations

0
Intracellular vacuoles induced by hypo-osmotic stress visualized by coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging DOI Creative Commons

Zuliang Hu,

Kyota Shirane,

Kota Aramaki

et al.

Applied Physics Express, Journal Year: 2024, Volume and Issue: 17(9), P. 092001 - 092001

Published: July 16, 2024

Abstract We analyzed the effects of a hypo-osmotic environment on rat Schwann cells, type glial cell surrounding neurons, using ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) microscopy. After treatment, we detected vacuole-like components in cytoplasm both bright-field and CARS spectroscopic imaging. An approach integrating morphological examination analysis based multiple vibrational bands revealed that these structures are predominantly water-filled, their characteristics closely resembled those vacuoles observed plant cells.

Language: Английский

Citations

0
Molecular Fingerprinting of Mouse Brain Using Ultrabroadband Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy Empowered by Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) DOI Creative Commons
Yusuke Murakami, Masahiro Ando,

Ayako Imamura

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(10), P. 689 - 697

Published: July 25, 2024

The Raman fingerprint spectral region provides abundant structural information on molecules. However, analyzing vibrational images within this using coherent imaging remains challenging due to the small cross section and congested features. In study, we combined ultrabroadband anti-Stokes scattering (CARS) microspectroscopy across range of 500-4000 cm

Language: Английский

Citations

0
Numerical modeling of partially coherent anti-Stokes Raman scattering (pCARS) in transparent molecular liquids DOI Creative Commons
Georgi B. Hadjichristov,

Boyko Katranchev

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: unknown, P. 125977 - 125977

Published: Sept. 1, 2024

Language: Английский

Citations

0
Fourier Transform Coherent Anti-Stokes Raman Scattering Spectroscopy: A Comprehensive Review DOI
Ryo Nishiyama,

Kei Furuya,

Tetsu Tamura

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(46), P. 18322 - 18336

Published: Oct. 22, 2024

Fourier transform coherent anti-Stokes Raman scattering (FT-CARS) spectroscopy is a powerful spectroscopic method that combines the principles of with (CARS). This stands out in for its ability to rapidly acquire spectra, achieving an impressive rate over 10 000 spectra per second. The involves scanning optical delay between two femtosecond pulses; initial pulse induces vibrational coherence sample, while subsequent probes this increasing delays. intensity generated modulated by dynamics enabling retrieval through transformation. Over past decades, FT-CARS has undergone substantial evolution, paving way application wide array fields, including material analysis and flow cytometry. In comprehensive Review, we explore fundamental diverse applications delve into potential future advances challenges associated emerging method.

Language: Английский

Citations

0