Electrochemiluminescence Microscopy

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(29)

Опубликована: Май 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Язык: Английский

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Photocatalysis on Hybrid Plasmonic Nanomaterials: From Catalytic Mechanism Study at Single-Particle Level to Materials Design

ACS Catalysis, Год журнала: 2024, Номер 14(15), С. 11425 - 11446

Опубликована: Июль 17, 2024

Plasmonic nanomaterials can convert low-intensity solar energy into chemical due to their surface plasmon resonance (SPR) effect, offering an interesting approach enhancing conversion efficiency. Unraveling the physicochemical mechanisms of hot carrier relaxation and precise design hybrid plasmonic nanostructures are crucial for optimizing potential SPR effect in photocatalysis, especially considering ongoing challenges low quantum efficiency controversial plasmon-enhanced reactions. Characterization analysis methods at single-particle level emerging as powerful tools achieving this objective. It reveal adsorbate–surface interactions, determine reliable structure–activity relationships individual nanoparticles, further analyze catalytic mechanisms. In review, we highlighted progression mechanism studies that include exploration interfacial charge transfer between nanoparticles with adsorber (metal, semiconductors, or molecule), imaging activity, evolution nanostructures, which provided guidance highly efficient nanomaterials. Finally, discuss future prospects field. This review aims offer insights photocatalysis by emphasizing level, goal expediting development high-performing photocatalysts.

Язык: Английский

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Unveiling the Dynamic Electrocatalytic Activity of Online Synthesized Bimetallic Nanocatalysts via Electrochemiluminescence Microscopy

Nano Letters, Год журнала: 2024, Номер 24(15), С. 4665 - 4671

Опубликована: Апрель 8, 2024

Effective bimetallic nanoelectrocatalysis demands precise control of composition, structure, and understanding catalytic mechanisms. To address these challenges, we employ a two-in-one approach, integrating online synthesis with real-time imaging Au@Metal core–shell nanoparticles (Au@M NPs) via electrochemiluminescence microscopy (ECLM). Within 120 s, electrodeposition in situ activity screening alternate. ECLM captures transient faradaic processes during potential switches, visualizes electrochemical real-time, tracks dynamics at the single-particle level. Analysis using ECL photon flux density eliminates size effects yields quantitative electrocatalytic results. Notably, nonlinear trend corresponding to shell metal Au surface atomic ratio is discerned, quantifying optimal component Au@M NPs. This approach offers comprehensive behavior deposition process high spatiotemporal resolution, which crucial for tailoring efficient nanocatalysts diverse applications.

Язык: Английский

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Enhanced Electrochemiluminescence at the Gas/Liquid Interface of Bubbles Propelled into Solution

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(32), С. 22724 - 22735

Опубликована: Авг. 2, 2024

Electrochemiluminescence (ECL) is typically confined to a micrometric region from the electrode surface. This study demonstrates that ECL emission can extend up several millimeters away employing electrogenerated chlorine bubbles. The mechanism behind this bubble-enhanced was investigated using an Au microelectrode in chloride-containing and chloride-free electrolyte solutions. We discovered at gas/solution interface driven by two parallel effects. First, bubble corona effect facilitates generation of hydroxyl radicals capable oxidizing luminol while attached Second, hypochlorite generated sustains for over 200 s extends range 5 mm into solution, following detachment. new approach increase intensity luminol-based assays 5-fold compared conventional method. demonstrated through glucose bioassay, midrange mobile phone camera detection. These findings significantly expand potential applications extending its effective time space.

Язык: Английский

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Ultrasensitive Electrochemiluminescence Biosensor with ZIF‐67@MXene as an Efficient Co‐Reaction Accelerator and Plasmonic Nanozyme as a Smart Signal Amplification Probe

Small, Год журнала: 2024, Номер unknown

Опубликована: Сен. 18, 2024

Abstract Exploring novel electrochemiluminescence (ECL) co‐reaction accelerators to construct ultrasensitive sensing systems is a prominent focus for developing advanced ECL sensors. However, challenges still remain in finding highly efficient and understanding their promoting mechanisms. In this paper, ZIF‐67@MXene nanosheet composites, with conductive in‐plane structure confined‐stable pore/channel, are designed act as high‐efficient achieve significant enhancement the luminol‐H 2 O based system. Mechanism investigation suggests that hydroxyl radicals (·OH) singlet oxygen ( 1 ) can be selectively preferentially generated on due stable absorption of ·OH , leading remarkable efficiency luminol (830%). Finally, by designing plasmonic NH ‐MIL‐88@Pd nanozyme, an “on‐off” switch immunosensor constructed detection prostate‐specific antigen (PSA). Based multiple signal amplification effect, linear range PSA expanded three orders magnitude. The limit also improved from 1.44 × 10 −11 9.1 −13 g mL −1 . This work proposes effective method preparation provides new strategy sensitive cancer markers.

Язык: Английский

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Light Conversion by Electrochemiluminescence at Semiconductor Surfaces

Accounts of Chemical Research, Год журнала: 2024, Номер 57(15), С. 2144 - 2153

Опубликована: Июль 17, 2024

ConspectusElectrochemiluminescence (ECL) is the electrochemical generation of light. It involves an interfacial charge transfer that produces excited state a luminophore at electrode surface. ECL powerful readout method widely employed for immunoassays and clinical diagnostics progressively evolving into microscopy technique. On other hand, photoelectrochemistry illuminated semiconductors field research deals with photogenerated carriers solid-liquid interface. This concept offers several advantages such as considerable lowering onset potential required triggering reaction well light addressable chemistry, via spatial confinement redox reactions locally semiconductor electrodes. The combination termed photoinduced (PECL). through minority solid/liquid PECL results in conversion incident photons (λ

Язык: Английский

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Infrared photoinduced electrochemiluminescence microscopy of single cells

Chemical Science, Год журнала: 2023, Номер 15(6), С. 2055 - 2061

Опубликована: Дек. 8, 2023

Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated an incident near-infrared light (λexc 1050 nm). The were grown on metal-insulator-semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. large anti-Stokes shift allowed for recording well-resolved images with high sensitivity. microscopy demonstrated at remarkably low onset potential 0.8 V; this contrasts classic ECL, which blind potential. Two modes are reported: (i) positive (PECL+), showing cell membranes labeled [Ru(bpy)3]2+ complex; (ii) shadow label-free (PECL-) morphology, luminophore in solution. Finally, adding new dimension stimulus, should find promising applications to image study photoactive nanoparticles biological entities.

Язык: Английский

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Photoinduced Electrochemiluminescence Immunoassays

Analytical Chemistry, Год журнала: 2024, Номер 96(45), С. 18262 - 18268

Опубликована: Ноя. 1, 2024

Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge the improvement operation conditions required bead-based using typical [Ru(bpy)3]2+/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates imaging and quantitative analysis [Ru(bpy)3]2+-modified immunobeads at low anodic potential. The photovoltage generated by photoanode under light promotes oxidation processes electrode/electrolyte interface, thus considerably lowering onset potential both TPrA ECL emission. anti-Stokes shift between excitation (invisible to human eyes) visible emitted results in clear stable signal from immunobeads. addition, it offers possibility site-selective photoexcitation process. This approach not only meets performance traditional accuracy but also additional benefits lower requirements enhanced stability, providing new perspective optimization commercial immunoassays.

Язык: Английский

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Near-IR Photoinduced Electrochemiluminescence Imaging with Structured Silicon Photoanodes

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(9), С. 11722 - 11729

Опубликована: Фев. 23, 2024

Infrared (IR) imaging devices that convert IR irradiation (invisible to the human eye) a visible signal are based on solid-state components. Here, we introduce an alternative concept light-addressable electrochemistry (i.e., spatially confined under action of light stimulus) involves use liquid electrolyte. In this method, projection near-IR image (λexc = 850 or 840 nm) onto photoactive Si-based photoanode, immersed into phase, triggers locally photoinduced electrochemiluminescence (PECL) efficient [Ru(bpy)3]2+-TPrA system. This leads local conversion (λPECL 632 light. We demonstrate compared planar Si photoanodes, micropillar array large enhancement generation and considerably improves resolution PECL by preventing photogenerated minority carriers from diffusing laterally. These results important for design original can lead applications in photothermal analytical chemistry.

Язык: Английский

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Ternary Heterojunction ZnS‐ZnIn₂S₄‐In₂S₃ Efficiently Catalyzes Triethylamine to Enhance Electrochemiluminescence Imaging Signals

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Abstract The visualization of electrode reactions is significant for ultra sensitive detection. Traditional electrochemiluminescence (ECL) detection method can hardly visualize reactions. Combining ECL imaging technology and traditional methods overcome the lack visual feedback key information in sensor construction. In contrast to cyclic voltammetry testing, applying a constant voltage surface results more stable signal. this study, methionine‐stabilized gold nanoclusters (Met‐AuNCs) are used as efficient near‐infrared luminescent clusters, triethylamine (TEA) coreactants construct an acetamiprid (ACT). ternary heterojunction ZnS‐ZnIn 2 S 4 ‐In 3 (Z‐Z‐I) forms stepwise electron transfer mode, facilitating rapid generation free radicals TEA. Furthermore, Z‐Z‐I serves substrate, providing large loading area DNA chain, which lays foundation wide range. Such has linear range (100 f m 1 µ ) achieves low limit (42 fM) ACT This study not only provides accurate detecting agricultural products but also foretells promising application electrochemiluminescent sensors.

Язык: Английский

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