Abstract
Intravital
fluorescence
imaging
in
the
second
near‐infrared
window
(NIR‐II,
900–1700
nm)
has
emerged
as
a
promising
method
for
non‐invasive
diagnostics
complex
biological
systems
due
to
its
advantages
of
less
background
interference,
high
tissue
penetration
depth,
contrast,
and
sensitivity.
However,
traditional
NIR‐II
imaging,
which
is
characterized
by
“always
on”
or
“turn
mode,
lacks
ability
quantitative
detection,
leading
low
reproducibility
reliability
during
bio‐detection.
In
ratiometric
can
realize
reliable
analysis
detection
vivo
providing
reference
signals
correction,
generating
new
opportunities
prospects
bioimaging
biosensing.
this
review,
current
design
strategies
sensing
mechanisms
probes
biosensing
applications
are
systematically
summarized.
Further,
challenges,
future
perspectives
designing
also
discussed.
It
hoped
that
review
provide
effective
guidance
promote
adoption
vivo.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Апрель 25, 2022
Afterglow
luminescence
is
an
internal
pathway
that
occurs
after
photo-excitation,
holds
great
promise
for
non-background
molecular
imaging
in
vivo,
but
suffer
from
poor
quantitative
ability
owing
to
luminescent
attenuation
over
time.
Moreover,
the
inert
structure
and
insufficient
reactive
sites
of
current
afterglow
materials
make
it
hard
design
activatable
probes
specific
detection.
Here,
we
report
a
ratiometric
nanoplatform
customize
various
reliable
quantification
analytes,
such
as
NO,
ONOO-
or
pH.
Notably,
these
can
not
only
address
intensity
eliminate
interference
factors
(e.g.,
laser
power,
irradiation
time,
exposure
time),
also
significantly
improve
reliability
vivo
signal-to-background
ratios
(~1200-fold),
both
which
enable
more
analysis
biological
systems.
proof-of-concept,
successfully
NO-responsive
nanoprobe,
RAN1.
This
nanoprobe
monitor
fluctuations
intratumoral
biomarker
macrophage
polarization,
making
possible
real-time
dynamically
evaluate
degree
cancer
immunotherapy,
provides
parameter
predict
immunotherapeutic
effect.
Chemical Reviews,
Год журнала:
2023,
Номер
124(2), С. 554 - 628
Опубликована: Ноя. 22, 2023
In
vivo
imaging
technologies
have
emerged
as
a
powerful
tool
for
both
fundamental
research
and
clinical
practice.
particular,
luminescence
in
the
tissue-transparent
near-infrared
(NIR,
700–1700
nm)
region
offers
tremendous
potential
visualizing
biological
architectures
pathophysiological
events
living
subjects
with
deep
tissue
penetration
high
contrast
owing
to
reduced
light–tissue
interactions
of
absorption,
scattering,
autofluorescence.
The
distinctive
quantum
effects
nanocrystals
been
harnessed
achieve
exceptional
photophysical
properties,
establishing
them
promising
category
luminescent
probes.
this
comprehensive
review,
between
light
tissues,
well
advantages
NIR
imaging,
are
initially
elaborated.
Subsequently,
we
focus
on
achieving
improved
by
optimizing
performance
nanocrystal
fluorophores.
ingenious
design
strategies
probes
discussed,
along
their
respective
biomedical
applications
versatile
modalities.
Finally,
thought-provoking
reflections
challenges
prospects
future
translation
nanocrystal-based
wisely
provided.
ACS Nano,
Год журнала:
2024,
Номер
18(5), С. 3916 - 3968
Опубликована: Янв. 23, 2024
Dynamic
variations
in
the
concentration
and
abnormal
distribution
of
endogenous
biomarkers
are
strongly
associated
with
multiple
physiological
pathological
states.
Therefore,
it
is
crucial
to
design
imaging
systems
capable
real-time
detection
dynamic
changes
for
accurate
diagnosis
effective
treatment
diseases.
Recently,
ratiometric
has
emerged
as
a
widely
used
technique
sensing
due
its
advantage
circumventing
limitations
inherent
conventional
intensity-dependent
signal
readout
methods
while
also
providing
built-in
self-calibration
correction.
Here,
recent
progress
probes
their
applications
outlined.
Ratiometric
classified
according
mechanisms,
photoacoustic
imaging,
optical
including
photoluminescence
self-luminescence
magnetic
resonance
dual-modal
discussed.
The
such
pH,
reactive
oxygen
species
(ROS),
nitrogen
(RNS),
glutathione
(GSH),
gas
molecules,
enzymes,
metal
ions,
hypoxia
discussed
detail.
Additionally,
this
Review
presents
an
overview
challenges
faced
field
along
future
research
directions.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(16)
Опубликована: Янв. 4, 2024
Abstract
Second
near‐infrared
(NIR‐II)
bioimaging
technology
shows
broad
application
prospects
in
the
field
of
biomedical
due
to
its
significant
superiority
deep
tissue
penetration
and
high
signal‐to‐background
ratio.
Among
them,
organic
small
molecule
fluorophores
have
received
progressively
more
consideration
their
low
biotoxicity,
biocompatibility,
rapid
clearance,
good
modification
properties.
As
key
acceptor
core,
2,
1,
3‐benzothiadiazole
(BTD)
derivative
plays
an
essential
role
construction
donor‐acceptor‐donor
type
NIR‐II
fluorophores.
This
mini‐review
summarizes
research
development
based
on
BTD
derivatives
bioimaging,
focusing
molecular
design
photophysical
properties,
introduces
a
part
applications
biological
field.
Furthermore,
challenges
BTD‐based
future
are
discussed
.
Abstract
Intravital
fluorescence
imaging
in
the
second
near‐infrared
window
(NIR‐II,
900–1700
nm)
has
emerged
as
a
promising
method
for
non‐invasive
diagnostics
complex
biological
systems
due
to
its
advantages
of
less
background
interference,
high
tissue
penetration
depth,
contrast,
and
sensitivity.
However,
traditional
NIR‐II
imaging,
which
is
characterized
by
“always
on”
or
“turn
mode,
lacks
ability
quantitative
detection,
leading
low
reproducibility
reliability
during
bio‐detection.
In
ratiometric
can
realize
reliable
analysis
detection
vivo
providing
reference
signals
correction,
generating
new
opportunities
prospects
bioimaging
biosensing.
this
review,
current
design
strategies
sensing
mechanisms
probes
biosensing
applications
are
systematically
summarized.
Further,
challenges,
future
perspectives
designing
also
discussed.
It
hoped
that
review
provide
effective
guidance
promote
adoption
vivo.
