Nano Research, Год журнала: 2021, Номер 15(2), С. 845 - 857
Опубликована: Июнь 16, 2021
Язык: Английский
ACS Nano, Год журнала: 2021, Номер 15(9), С. 14253 - 14262
Опубликована: Авг. 19, 2021
As a powerful signal amplification tool, the DNA walker has been widely applied to detect rare microRNA (miRNA) in vivo. Despite significant advances, near-infrared (NIR) light controllable for powered by an endogenous initiator not realized, which is crucial spatiotemporal imaging of miRNA living cells with high sensitivity. Herein, we constructed NIR-photoactivatable system, was adenosine triphosphate (ATP) situ spatial and temporal resolution. The system very stable extremely low fluorescent background bioimaging cells. We employed upconversion nanoparticles (UCNPs) as carriers probe transducers converting NIR UV light. Coupled fueled intracellular ATP, smart based on initiated successfully developed precise control Triggered light, could autonomously progressively travel along track assistance ATP. different cell lines highly This strategy can expand photocontrol biological system.
Язык: Английский
Процитировано
109
Angewandte Chemie International Edition, Год журнала: 2021, Номер 60(33), С. 17937 - 17941
Опубликована: Июнь 12, 2021
Abstract MicroRNA (miRNA) functions are tightly regulated by their sub‐compartmental location in living cells, and the ability to imaging of mitochondrial miRNAs (mitomiRs) is essential for understanding related pathological processes. However, most existing DNA‐based methods could not be used this purpose. Here, we report development a DNA nanoreporter technology mitomiRs cells through near‐infrared (NIR) light‐controlled strand displacement reactions. The sensing function nanoreporters silent (OFF) during delivery process, but can photoactivated (ON) with NIR light after targeted localization, enabling spatially‐restricted two types cancer‐related improved detection accuracy. Furthermore, demonstrate vivo spatiotemporally‐controlled activation. Therefore, study illustrates simple methodology that may broadly applicable investigating mitomiRs‐associated physiological events.
Язык: Английский
Процитировано
105
Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(32)
Опубликована: Июнь 6, 2022
DNA-based biosensor technologies have shown great potential in chemical and biological detection. These biosensors been actively developed as probes for molecular imaging live cells animals, allowing situ detection of analytes complex systems, elucidation the roles key molecules processes, development non-invasive diagnosis image-guided surgery. Despite progress made, improving spatial-temporal precision remains a challenge this field. In Minireview, we describe concepts behind spatiotemporally selective via combination engineered, light-activatable upconversion nanotechnology. We then highlight application approach controlled various targets specific intracellular organelles, signal amplification, well regulation targeting activity to receptor proteins. finally discuss challenges perspectives possible future developments emerging
Язык: Английский
Процитировано
54
Acta Biomaterialia, Год журнала: 2023, Номер 166, С. 42 - 68
Опубликована: Май 29, 2023
Язык: Английский
Процитировано
37
Analytical Chemistry, Год журнала: 2023, Номер 95(24), С. 9271 - 9279
Опубликована: Июнь 6, 2023
A novel homologous surface-enhanced Raman scattering (SERS)-electrochemical (EC) dual-mode biosensor based on a 3D/2D polyhedral Au nanoparticle/MoOx nanosheet heterojunction (PAMS HJ) and target-triggered nonenzyme cascade autocatalytic DNA amplification (CADA) circuit was constructed for highly sensitive detection of microRNA (miRNA). Mixed-dimensional heterostructures were prepared by in situ growth nanoparticles (PANPs) the surface MoOx nanosheets (MoOx NSs) via seed-mediated method. As substrate, resulting PAMS HJ shows synergistic effects both electromagnetic chemical enhancements, efficient charge transfer, robust stability, thus achieving high SERS enhancement factor (EF) 4.2 × 109 strong EC sensing performance. Furthermore, molecular recognition between target smart lock probe gradually accelerated reaction further improved selectivity sensitivity our platform. The limits miRNA-21 mode 0.22 2.69 aM, respectively. More importantly, proposed platform displayed excellent anti-interference accuracy analysis human serum cell lysates, indicating its potential as reliable tool field biosensing clinical analysis.
Язык: Английский
Процитировано
24
Science Advances, Год журнала: 2024, Номер 10(30)
Опубликована: Июль 24, 2024
An ortho-nitrobenzyl phosphate ester-caged nucleic acid hairpin structure coupled to the CRISPR-Cas12a complex is introduced as a functional reaction module for light-induced activation of (LAC12a) machinery toward amplified fluorescence detection microRNA-21 (miRNA-21). The LAC12a applied selective, in vitro sensing miRNA-21 and intracellular imaging different cell lines. system used image cycle phases MCF-7 cells. Moreover, integrated cells enables two-photon laser confocal microscopy-assisted, light-stimulated spatiotemporal, selective at single-cell level evaluation relative expression levels distinct phases. method implemented map distribution an array single
Язык: Английский
Процитировано
12
Materials Today Advances, Год журнала: 2022, Номер 14, С. 100219 - 100219
Опубликована: Фев. 16, 2022
Female breast cancer is the leading cause of global incidence and fifth mortality worldwide in 2020. Breast can be categorized into different subtypes according to expression biomarkers such as estrogen receptors (ER), progesterone (PR) human epidermal growth factor receptor 2 (HER2). The analysis these also indispensable for systemic treatment decisions cancer. In this review, we summarized recent progress detection blood utilizing nanomaterials. attractive physical, chemical, optical electronic properties various nanomaterials facilitated improved performance detection. relatively non-invasive provide useful biological information that complementary imaging examination immunohistochemistry.
Язык: Английский
Процитировано
33
Science China Chemistry, Год журнала: 2023, Номер 66(11), С. 3105 - 3115
Опубликована: Окт. 7, 2023
Язык: Английский
Процитировано
18
Advanced Sensor Research, Год журнала: 2023, Номер 2(9)
Опубликована: Март 3, 2023
Abstract Probing endogenous molecules in living entities is significant to help decipher biological functions and exploit novel theranostics. DNA circuits that can recognize molecular inputs of interest transduce them into readable signal outputs an isothermal autonomous manner have been actively pursued as versatile toolkits for intracellular biosensing research. Tremendous efforts are being devoted developing integrated with high sensitivity, while spatiotemporal selectivity often overlooked the construction functional circuitry systems. This requires development stimuli‐responsive be activated on‐demand from initial sensing‐blunt state sensing‐ready under a programmable manner, achieving precise bioimaging control. In this review, overview recent advances respond particular triggers, including external physical stimuli cues, their spatiotemporally controllable applications provided. The current challenges potential solutions these future developments emerging field also discussed.
Язык: Английский
Процитировано
17
ACS Applied Materials & Interfaces, Год журнала: 2021, Номер 13(35), С. 41498 - 41506
Опубликована: Авг. 26, 2021
Covalent organic frameworks (COFs) have emerged as promising materials for biomedical applications, but their functions remain to be explored and the potential toxicity concerns should resolved. Herein, it is presented that carbonization significantly enhances fluorescence quenching efficiency aqueous stability of nanoscale COFs. The probes prepared by physisorbing dye-labeled nucleic acid recognition sequences onto carbonized COF nanoparticles (termed C-COF) were employed cell imaging, which could effectively light up biomarkers (survivin TK1 mRNA) in living cells. C-COF has enhanced photothermal conversion capacity, indicating are also candidates therapy. concern from aromatic rigid building units COFs was detoured carbonization. Overall, a strategy developing biocompatible multifunctional COF-derived nanoprobes applications. This work may inspire more versatile bioanalysis nanomedicine.
Язык: Английский
Процитировано
39