DNA Walkers for Biosensing Development

Advanced Science, Год журнала: 2022, Номер 9(18)

Опубликована: Апрель 22, 2022

Abstract The ability to design nanostructures with arbitrary shapes and controllable motions has made DNA nanomaterials used widely construct diverse nanomachines various structures functions. exhibit excellent properties, including programmability, stability, biocompatibility, can be modified different functional groups. Among these nanoscale architectures, walker is one of the most popular nanodevices ingenious flexible function. In past several years, walkers have amazing progress ranging from structural biological applications constructing biosensors for detection cancer‐associated biomarkers. this review, key driving forces are first summarized. Then, numbers legs introduced. Furthermore, biosensing detection‐ nucleic acids, proteins, ions, bacteria Finally, new frontiers opportunities developing walker‐based discussed.

Язык: Английский

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Metal-organic framework nanoreactor-based electrochemical biosensor coupled with three-dimensional DNA walker for label-free detection of microRNA

Biosensors and Bioelectronics, Год журнала: 2022, Номер 207, С. 114188 - 114188

Опубликована: Март 15, 2022

Язык: Английский

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Construction of Fast-Walking Tetrahedral DNA Walker with Four Arms for Sensitive Detection and Intracellular Imaging of Apurinic/Apyrimidinic Endonuclease 1

Analytical Chemistry, Год журнала: 2022, Номер 94(24), С. 8732 - 8739

Опубликована: Июнь 9, 2022

Herein, a novel tetrahedral DNA walker with four arms was engineered to travel efficiently on the 3D-tracks via catalyzed hairpin assembly autonomously, realizing sensitive detection and activity assessment as well intracellular imaging of apurinic/apyrimidinic endonuclease 1 (APE1). In contrast traditional walkers, rigid 3D framework structure nonplanar multi-sites walking endowed high collision efficiency, showing fast rate nuclease resistance. Impressively, initial 4.54 times faster than that free bipedal produced significant fluorescence recovery in about 40 min, achieving APE1 low limit 5.54× 10–6 U/μL ultrasensitive activation imaging. This strategy provides for accurate identification low-abundance cancer biomarker potential medical diagnosis.

Язык: Английский

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Spatiotemporally Selective Molecular Imaging via Upconversion Luminescence‐Controlled, DNA‐Based Biosensor Technology

Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(32)

Опубликована: Июнь 6, 2022

DNA-based biosensor technologies have shown great potential in chemical and biological detection. These biosensors been actively developed as probes for molecular imaging live cells animals, allowing situ detection of analytes complex systems, elucidation the roles key molecules processes, development non-invasive diagnosis image-guided surgery. Despite progress made, improving spatial-temporal precision remains a challenge this field. In Minireview, we describe concepts behind spatiotemporally selective via combination engineered, light-activatable upconversion nanotechnology. We then highlight application approach controlled various targets specific intracellular organelles, signal amplification, well regulation targeting activity to receptor proteins. finally discuss challenges perspectives possible future developments emerging

Язык: Английский

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Self-Powered DNAzyme Walker Enables Dual-Mode Biosensor Construction for Electrochemiluminescence and Electrochemical Detection of MicroRNA

Analytical Chemistry, Год журнала: 2023, Номер 95(17), С. 7006 - 7013

Опубликована: Апрель 21, 2023

Herein, an electrochemiluminescence (ECL) and electrochemical (EC) dual-mode biosensor platform with a self-powered DNAzyme walking machine was established for accurate sensitive detection of miRNA-21. By employing magnesium ion (Mn2+)-dependent cleavage cycling reaction, the built by assembling strands ferrocene (Fc)-labeled substrate on Au nanoparticles graphitic carbon nitride nanosheet (g-C3N4 NS)-covered electrode. The strand first prohibited blocker strand. After addition target miRNA-21 Mn2+, walker could be activated produce autonomous movements along electrode track fueled Mn2+-dependent DNAzyme-catalyzed without additional energy supply. Notably, each step resulted in release Fc-labeled DNA fragment, allowing us to acquire extreme ECL signal recovery g-C3N4 inhibited Fc. Meanwhile, numerous fragments escaped from surface electrode, directly producing obvious decrease square wave voltammetry (SWV) Fc same sensing platform. This work not only avoided difficultly various indicators but also significantly improved sensitivity through using DNAzyme-walker amplification. Moreover, proposed design employed reaction two output modes, which eliminate interference diverse reactive pathways outcome mutually improve accuracy. Therefore, exhibited wide ranges 100 aM nM low limits 54.3 78.6 SWV respectively, provided efficient universal biosensing approach extensive applications early disease diagnosis bioanalysis.

Язык: Английский

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High-Efficiency 3D DNA Walker Immobilized by a DNA Tetrahedral Nanostructure for Fast and Ultrasensitive Electrochemical Detection of MiRNA

Analytical Chemistry, Год журнала: 2023, Номер 95(8), С. 4077 - 4085

Опубликована: Фев. 14, 2023

Herein, by directly limiting the reaction space, an ingenious three-dimensional (3D) DNA walker (IDW) with high walking efficiency is developed for rapid and sensitive detection of miRNA. Compared traditional walker, IDW immobilized tetrahedral nanostructure (DTN) brings stronger kinetic thermodynamic favorability resulting from its improved local concentration space confinement effect, accompanied a quite faster speed much better efficiency. Once traces target miRNA-21 react prelocked IDW, could be largely activated walk on interface electrode to trigger cleavage H2 assistance Mg2+, in release amounts methylene blue (MB) labeled surface obvious decrease signal. Impressively, reveals conversion as 9.33 × 108 30 min fast speed, which at least five times beyond that typical walkers. Therefore, address inherent challenges easily: slow low Notably, nanomachine was utilized construct sensing platform limit (LOD) 19.8 aM realize highly assay biomarker total RNA lysates cancer cell. The strategy thus helps design versatile nucleic acid signal amplification approach practical applications areas biosensing assay, nanotechnology, clinical diagnosis.

Язык: Английский

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Well-Aligned Track-Accelerated Tripedal DNA Walker for Photoelectrochemical Recognition of Dual-miRNAs Based on Molecular Logic Gates

Analytical Chemistry, Год журнала: 2023, Номер 95(13), С. 5764 - 5772

Опубликована: Март 24, 2023

Post-transcriptional regulators, microRNAs (miRNAs), are involved in the occurrence and progression of various diseases. However, due to complexity disease-related miRNA regulatory networks, typing identification miRNAs have remained challenging. Herein, a linear ladder-like DNA nanoarchitecture (LDN) was constructed promote movement efficiency tripedal walker (T-walker), which combined with DNA-based logic gates PTCDA@PDA/CdS/WO3 photoelectrode develop novel biosensor for detection dual-miRNAs. Two miRNAs, miR-122 miR-21, were used as targets operate module, while its output, trigger strands (TSs), initiated catalytic hairpin assembly (CHA) reaction form T-walker. By using LDN track, T-walker efficiently unfolded 4, further hybridized alkaline phosphatase-modified 5 (AP-H5). The remaining AP can catalyze ascorbic acid 2-phosphate (AAP) into (AA), an ideal electron donor, thus resulting photocurrent change. signals both AND OR displayed relationship logarithm dual-miRNA concentrations limits 10.1 13.6 fM, respectively. Moreover, intelligent rational design tracks gives impetus create well-organized sensing interface wide application clinical diagnosis cancer monitoring.

Язык: Английский

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A NIR Programmable In Vivo miRNA Magnifier for NIR‐II Imaging of Early Stage Cancer

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(50)

Опубликована: Окт. 31, 2023

Aberrant expressions of biomolecules occur much earlier than tumor visualized size and morphology change, but their common measurement strategies such as biopsy suffer from invasive sampling process. In vivo imaging slight biomolecule expression difference is urgently needed for early cancer detection. Fluorescence rare earth nanoparticles (RENPs) in second near-infrared (NIR-II) region makes them appropriate tool imaging. However, the incapacity to couple with signal amplification strategies, especially programmable limited application lowly expressed biomarkers Here we develop a 980/808 nm NIR programmed microRNAs (miRNAs) magnifier by conjugating activatable DNAzyme walker set RENPs, which achieves more effective NIR-II stage monitoring technique. Dye FD1080 (FD1080) modified substrate DNA quenches downconversion emission RENPs under 808 excitation. The miRNA recognition sealed photo-cleavable strand avoid "false positive" systemic circulation. Upconversion 980 irradiation activates amplifies fluorescence recovery via catalytic reaction achieve This strategy demonstrates good potential field

Язык: Английский

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A photoelectrochemical biosensor based on ZnIn2S4@AuNPs coupled with circular bipedal DNA walker for signal-on detection of circulating tumor DNA

Biosensors and Bioelectronics, Год журнала: 2023, Номер 231, С. 115295 - 115295

Опубликована: Апрель 5, 2023

Язык: Английский

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Advances in DNA walking nanomachine‐based biosensors

Deleted Journal, Год журнала: 2024, Номер 2(1)

Опубликована: Янв. 1, 2024

Abstract The field of DNA nanotechnology has evolved beyond the realm controllable movements and randomly shaped nanostructures, now encompassing a diverse array nanomachines, each with unique nanostructures biofunctional attributes. These boast exceptional characteristics such as programmability, integrability, biocompatibility, universality. Among this variety, walking nanomachines have emerged one most prominent nanomotors, distinguished by their ingenious design comprehensive functionality. In recent times, these walkers witnessed remarkable advancements in areas ranging from nanostructural designs to biological applications, including creation sophisticated biosensors capable efficiently detecting tumor‐related biomarkers bioactive substances. This review delves into operational mechanisms which are driven processes protease DNAzyme action well strand displacement photoactivated reactions. It further provides overview different dimensional (1D, 2D, 3D) tracks. A subsequent section introduces biosensing applications electrochemical, optical, other biosensors. concludes forward‐looking perspective on novel challenges developing nanomachine‐based

Язык: Английский

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