All-In-One Entropy-Driven DNA Nanomachine for Tumor Cell-Selective Fluorescence/SERS Dual-Mode Imaging of MicroRNA

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

An entropy-driven catalysis (EDC) strategy is appealing for amplified bioimaging of microRNAs in living cells; yet, complex operation procedures, lacking cell selectivity, and insufficient accuracy hamper its further applications. Here, we introduce an ingenious all-in-one DNA nanomachine (termed as AIO-EDN), which can be triggered by endogenous apurinic/apyrimidinic endonuclease 1 (APE1) to achieve tumor cell-selective dual-mode imaging microRNA. Compared with the traditional EDC strategy, integrated design AIO-EDN achieves autocatalytic signal amplification without extra fuel strands. Moreover, leverages APE1 overexpressed cancer cells activate reaction, which, however, exerts no target sensing activity normal cells. Combining fluorescence- surface-enhanced Raman scattering (FL/SERS) techniques, this exhibits significantly improved selectivity microRNA This study provides a new paradigm develop EDC-based platform shows great potential in-depth diagnosis high precision.

Язык: Английский

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Space-coded microchip for multiplexed respiratory virus detection via CRISPR-Cas12a and RPA

Talanta, Год журнала: 2025, Номер 291, С. 127815 - 127815

Опубликована: Фев. 27, 2025

Язык: Английский

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Endogenous HClO-Gated Cascade MicroRNA Imaging for Precise Diagnosis of Atherosclerosis In Vivo

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Precise imaging of atherosclerotic plaques using biomarkers could prompt the diagnosis and clinical management atherosclerosis (AS)-driven cardiovascular diseases. MicroRNA-155 (miR-155) plays a critical role in AS development, with its expression notably upregulated foam cells within plaques. However, miRNA methods for face significant challenges, including low specificity, inefficient delivery, poor cell selectivity. Herein, we develop an endogenous hypochlorous acid (HClO)-gated cascade signal amplification strategy precise miR-155 living cells, enabling accurate vivo ex detection This utilizes phosphorothioate (PT)-modified hairpin probe that is specifically deprotected by HClO uncaged miR-155, triggering catalytic assembly (CHA) to amplify fluorescence signals. The PT-CHA probes are encapsulated lipid nanoparticles (LNs), followed conjugating phosphatidylserine (PS)-binding peptide (PBP) selectively targeting intensity PT-CHA@LN-PBP aorta region shows clear differentiation among AS-bearing mice, miR-155-/- healthy mice. Moreover, strongly correlates plaque area progression can discriminate vulnerability risk under curve (AUC) 0.94. Imaging human aortic tissues further validates probe's capacity distinguish from normal endarterium. These findings establish as noninvasive, reliable diagnostic tool assessment AS.

Язык: Английский

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Leveraging Synthetic Antibody–DNA Conjugates to Expand the CRISPR-Cas12a Biosensing Toolbox

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

We report here the use of antibody-DNA conjugates (Ab-DNA) to activate collateral cleavage activity CRISPR-Cas12a enzyme. Our findings demonstrate that Ab-DNA effectively trigger CRISPR-Cas12a, enabling transduction antibody-mediated recognition events into fluorescence outputs. developed two different immunoassays using an as activator Cas12a: CRISPR-based immunosensing assay (CIA) for detecting SARS-CoV-2 spike S protein, which shows superior sensitivity compared with traditional enzyme-linked immunosorbent (ELISA), and immunomagnetic (CIMA). Notably, CIMA successfully detected protein in undiluted saliva a limit detection (LOD) 890 pM 2 h assay. results underscore benefits integrating Cas12a-based signal amplification antibody methods. The potential conjugates, combined CRISPR technology, offers promising alternative conventional enzymes used could facilitate development versatile analytical platforms non-nucleic acid targets.

Язык: Английский

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Photoactivatable CRISPR/Cas9 lateral flow strip platform for one-pot rapid detection of squamous cell carcinoma antigen DNA in blood

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137607 - 137607

Опубликована: Март 1, 2025

Язык: Английский

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Structure-switching G-quadruplex: An efficient CRISPR/Cas12a signal reporter for label-free colorimetric biosensing

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 307, С. 142410 - 142410

Опубликована: Март 22, 2025

Язык: Английский

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Integration of MicroRNAs with nanomedicine: tumor targeting and therapeutic approaches

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Апрель 7, 2025

MicroRNAs (miRNAs) are small, non-coding RNA molecules that play a pivotal role in the post-transcriptional regulation of gene expression. Over past decade, they have emerged as key regulators cancer progression, influencing different cellular processes such proliferation, apoptosis, metastasis, and immune evasion. Their unique ability to target multiple genes simultaneously makes miRNAs highly attractive potential therapeutic agents oncology. However, several challenges hindered their direct clinical application, most notably inherent instability biological fluids, rapid degradation by nucleases, inefficient delivery specific tumor sites. Additionally, off-target effects for toxicity further complicate use miRNAs. Nanomedicine offers promising solution these enabling development advanced platforms stable, safe, targeted Nanoparticle-based systems, liposomes, polymeric nanoparticles, inorganic nanocarriers, can protect from degradation, improve bioavailability, allow precise targeting through passive or active mechanisms. These nanocarriers also be engineered release response stimuli within microenvironment, enhancing efficacy while minimizing side effects. This review will explore integration with nanotechnology, focusing on various nanoparticle formulations roles miRNA stability, specificity, function treatment. In addition, we discuss current advances preclinical applications, highlight tumor-targeting strategies, address remaining toxicity, immunogenicity, scalability. Future research should focus overcoming barriers, ultimately paving way widespread adoption personalized miRNA-based nanomedicine therapy.

Язык: Английский

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Random Sanitization in DNA Information Storage Using CRISPR-Cas12a

Journal of the American Chemical Society, Год журнала: 2024, Номер unknown

Опубликована: Дек. 10, 2024

DNA information storage provides an excellent solution for metadata due to its high density, programmability, and long-term stability. However, current research primarily focuses on the processes of storing reading data, lacking comprehensive solutions secure wiping. Herein, we present a method random sanitization in using CRISPR-Cas12a (RSDISC) based precise control thermodynamic energy primer-template hybridization. We utilize collateral cleavage (trans-activity) single-stranded (ssDNA) by achieve selective files metadata. This enables ssDNA degradation with different GC contents, lengths, secondary structures efficiency up 99.9% 28,258 oligonucleotides within one round. demonstrate that number erasable could reach 10

Язык: Английский

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Random Sanitization in DNA information storage using CRISPR-Cas12a

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 5, 2024

Abstract DNA information storage provides an excellent solution for metadata due to its high density, programmability, and long-term stability. However, current research in primarily focuses on the processes of storing reading data, lacking comprehensive solutions secure wiping. Herein, we present a method random sanitization using CRISPR-Cas12a (RSDISC) based precise control thermodynamic energy primer-template hybridization. We utilize collateral cleavage (trans-activity) single-stranded (ssDNA) by achieve selective files metadata. This enables ssDNA degradation with different GC content, lengths, secondary structures efficiency up 99.9% 28,258 oligonucleotides within one round. demonstrate that number erasable could reach 10 11.7 model hybridization efficiency. Overall, RSDISC approach set foundation encryption, file classification, memory deallocation accurate data storage.

Язык: Английский

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SlipChip Enables the Integration of CRISPR-Cas12a and RPA for Fast and Stand-Alone HPV Detection

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

Human papillomavirus (HPV) screening is vital for the early detection and prevention of cervical cancer. However, existing methods often face challenges related to speed, simplicity, multiplexing, especially in resource-limited settings. Here we developed a portable SlipChip-based multiplexed rapid nucleic acid testing platform, named SMART, designed simultaneously detect HPV16 HPV18. SMART allows seamless integration RPA Cas12a assays on SlipChip includes heating membrane regulate on-chip assay temperatures. This operate as stand-alone platform without additional control instruments. The also features an All-in-One imaging mode data acquisition, enhancing its performance. enables sensitive HPV18 DNA across multiple samples just 36 min with limit approximately 6 copies per reaction. Testing 56 clinical at risk HPV infection validated SMART's performance, showing 97.7% sensitivity 100% specificity. In summary, offers system capable rapidly distinguishing between two most harmful subtypes, showcasing significant potential rapid, various applications.

Язык: Английский

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