Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

ACS Nano, Год журнала: 2023, Номер 17(15), С. 14205 - 14228

Опубликована: Июль 27, 2023

The use of nanoparticles (NPs) in nanomedicine holds great promise for the treatment diseases which conventional therapies present serious limitations. Additionally, NPs can drastically improve early diagnosis and follow-up many disorders. However, to harness their full capabilities, they must be precisely designed, produced, tested relevant models. Microfluidic systems simulate dynamic fluid flows, gradients, specific microenvironments, multiorgan complexes, providing an efficient cost-effective approach both synthesis screening. technologies allow under controlled conditions, enhancing batch-to-batch reproducibility. Moreover, due versatility microfluidic devices, it is possible generate customize endless platforms rapid vitro vivo screening NPs' performance. Indeed, devices show potential as advanced small organism manipulation immobilization. In this review, first we summarize major that synthesis. Next, will discuss most innovative enable mimicking environments well give insights into organism-on-a-chip promising application We conclude review with a critical assessment current challenges future directions impact field nanomedicine.

Язык: Английский

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Cancer Metastasis‐on‐a‐Chip for Modeling Metastatic Cascade and Drug Screening

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(21)

Опубликована: Янв. 15, 2024

Microfluidic chips are valuable tools for studying intricate cellular and cell-microenvironment interactions. Traditional in vitro cancer models lack accuracy mimicking the complexities of vivo tumor microenvironment. However, cancer-metastasis-on-a-chip (CMoC) combine advantages 3D cultures microfluidic technology, serving as powerful platforms exploring mechanisms facilitating drug screening. These able to compartmentalize metastatic cascade, deepening understanding its underlying mechanisms. This article provides an overview current CMoC models, focusing on distinctive that simulate invasion, intravasation, circulation, extravasation, colonization, their applications Furthermore, challenges faced by technologies discussed, while promising future directions research. The ongoing development integration these into studies expected drive transformative advancements field.

Язык: Английский

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Advances in Microfluidic‐Based Core@Shell Nanoparticles Fabrication for Cancer Applications

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Май 13, 2024

Current research in cancer therapy focuses on personalized therapies, through nanotechnology-based targeted drug delivery systems. Particularly, controlled release with nanoparticles (NPs) can be designed to safely transport various active agents, optimizing specific organs and tumors, minimizing side effects. The use of microfluidics (MFs) this field has stood out against conventional methods by allowing precise control over parameters like size, structure, composition, mechanical/biological properties nanoscale carriers. This review compiles applications the production core-shell NPs (CSNPs) for therapy, discussing versatility inherent microchannel and/or micromixer setups showcasing how these utilized individually or combination, as well technology allows development new advances more efficient fabrication nanoformulations. Recent biological studies have achieved an effective, safe, otherwise unreliable encapsulants such small interfering RNA (siRNA), plasmid DNA (pDNA), cisplatin a result precisely tuned nanocarriers, showing that is paving way innovative strategies nanofabrication, characterized continuous high reproducibility. Finally, analyzes technical, biological, technological limitations currently prevent from becoming standard.

Язык: Английский

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Classical and emerging approximations for the screening of antimicrobial peptide libraries

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 195 - 232

Опубликована: Янв. 1, 2025

Язык: Английский

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Nanotheranostics Revolutionizing Gene Therapy: Emerging Applications in Gene Delivery Enhancement

Journal of Nanotheranostics, Год журнала: 2025, Номер 6(2), С. 10 - 10

Опубликована: Апрель 9, 2025

Nanotheranostics—where nanoscale materials serve both diagnostic and therapeutic functions—are rapidly transforming gene therapy by tackling critical delivery challenges. This review explores the design engineering of various nanoparticle systems (lipid-based, polymeric, inorganic, hybrid) to enhance stability, targeting, endosomal escape genetic payloads. We discuss how real-time imaging capabilities integrated into these platforms enable precise localization controlled release genes, improving treatment efficacy while reducing off-target effects. Key strategies overcome barriers (such as proton sponge effect photothermal disruption) achieve nuclear are highlighted, along with recent advances in stimuli-responsive that facilitate spatiotemporal control expression. Clinical trials preclinical studies demonstrate expanding role nanotheranostics managing cancer, inherited disorders, cardiovascular neurological diseases. further address regulatory manufacturing hurdles must be for widespread clinical adoption nanoparticle-based therapies. By synthesizing progress ongoing challenges, this underscores transformative potential effective, targeted, image-guided delivery.

Язык: Английский

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Zweifach–Fung Microfluidic Device for Efficient Microparticle Separation: Cost-Effective Fabrication Using CO2 Laser-Ablated PMMA

Micromachines, Год журнала: 2024, Номер 15(7), С. 932 - 932

Опубликована: Июль 22, 2024

Microfluidic separators play a pivotal role in the biomedical and chemical industries by enabling precise fluid manipulations. Traditional fabrication of these devices typically requires costly cleanroom facilities, which limits their broader application. This study introduces novel microfluidic device that leverages passive Zweifach–Fung principle to overcome financial barriers. Through Lagrangian computational simulations, we optimized an eleven-channel configuration achieved perfect 100% recall rate for particles following specified normal distribution. Experimental evaluations determined 2 mL/h as optimal total flow (TFR), under showcased exceptional performance enhancements precision micrometer-sized particles, achieving overall accuracy 94% ± 3%. Fabricated using cost-effective, non-cleanroom method, this approach represents significant shift from conventional practices, dramatically reducing production costs while maintaining high operational efficacy. The cost each chip is less than USD 0.90 cents manufacturing process takes only 15 min. development not makes technology more accessible but also sets new standard future advancements field.

Язык: Английский

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Low-cost inertial microfluidic device for microparticle separation: A laser-Ablated PMMA lab-on-a-chip approach without a cleanroom

HardwareX, Год журнала: 2023, Номер 16, С. e00493 - e00493

Опубликована: Ноя. 12, 2023

Although microparticles are frequently used in chemistry and biology, their effectiveness largely depends on the homogeneity of particle size distribution. Microfluidic devices to separate purify particles based have been developed, but many require expensive cleanroom manufacturing processes. A cost-effective, passive microfluidic separator is presented, capable efficiently sorting purifying spanning range 15 µm 40 µm. Fabricated from Polymethyl Methacrylate (PMMA) substrates using laser ablation, this device circumvents need for facilities. Prior fabrication, rigorous optimization device's design was carried out through computational simulations conducted COMSOL Multiphysics. To gauge its performance, chitosan were employed as a test case. The results notably promising, achieving precision 96.14 %. This quantitative metric underscores size-based separation. low-cost accessible offers pragmatic solution laboratories researchers seeking precise control over sizes, without constraints environments. innovation not only mitigates limitations tied traditional cleanroom-based fabrication also widens horizons various applications within realms biology.

Язык: Английский

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Recent Advances in Polymer Science and Fabrication Processes for Enhanced Microfluidic Applications: An Overview

Micromachines, Год журнала: 2024, Номер 15(9), С. 1137 - 1137

Опубликована: Сен. 6, 2024

This review explores significant advancements in polymer science and fabrication processes that have enhanced the performance broadened application scope of microfluidic devices. Microfluidics, essential biotechnology, medicine, chemical engineering, relies on precise fluid manipulation micrometer-sized channels. Recent innovations materials, such as flexible, biocompatible, structurally robust polymers, been pivotal developing advanced systems. Techniques like replica molding, microcontact printing, solvent-assisted injection 3D printing are examined, highlighting their advantages recent developments. Additionally, discusses diverse applications polymer-based devices biomedical diagnostics, drug delivery, organ-on-chip models, environmental monitoring, industrial processes. paper also addresses future challenges, including enhancing resistance, achieving multifunctionality, ensuring biocompatibility, scaling up production. By overcoming these potential for widespread adoption impactful use technologies can be realized.

Язык: Английский

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Enhancing Magnetic Micro- and Nanoparticle Separation with a Cost-Effective Microfluidic Device Fabricated by Laser Ablation of PMMA

Micromachines, Год журнала: 2024, Номер 15(8), С. 1057 - 1057

Опубликована: Авг. 22, 2024

Superparamagnetic iron oxide micro- and nanoparticles have significant applications in biomedical chemical engineering. This study presents the development evaluation of a novel low-cost microfluidic device for purification hyperconcentration these magnetic particles. The device, fabricated using laser ablation polymethyl methacrylate (PMMA), leverages precise control over fluid dynamics to efficiently separate particles from non-magnetic ones. We assessed device’s performance through Multiphysics simulations empirical tests, focusing on separation magnetite blue carbon dots microparticles polystyrene at various total flow rates (TFRs). For nanoparticle separation, achieved recall up 93.3 ± 4% precision 95.9 1.2% an optimal TFR 2 mL/h, significantly outperforming previous models, which only 50% recall. Microparticle demonstrated accuracy 98.1 1% mL/h both experimental conditions. Lagrangian model effectively captured microparticle microparticles, with close agreement between simulated results. Our findings underscore robust capability distinguishing nanoscales. highlights potential low-cost, non-cleanroom manufacturing techniques produce high-performance devices, thereby expanding their accessibility applicability industrial research settings. integration continuous magnet, as opposed segmented magnets designs, was identified key factor enhancing efficiency.

Язык: Английский

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Enrichment of T-lymphocytes from leukemic blood using inertial microfluidics toward improved chimeric antigen receptor-T cell manufacturing

Cytotherapy, Год журнала: 2024, Номер 26(10), С. 1264 - 1274

Опубликована: Май 10, 2024

Chimeric antigen receptor (CAR-T) cell therapy is a successful immunotherapy for treatment of blood cancers. However, hurdles in their manufacturing remain including efficient isolation and purification the T-cell starting material. Herein, we describe one-step separation based on inertial spiral microfluidics enrichment T-cells B-cell acute lymphoblastic leukemia (ALL) chronic lymphocytic (CLL) patient's samples. In healthy donors used to optimise process, lymphocyte purity was enriched from 65% (SD±0.2) 91% (SD±0.06) 45% (SD±0.1) 73% (SD±0.02). Leukemic samples had higher compared donor Efficient recovery lymphocytes achieved ALL with B-cells, monocytes leukemic blasts depleted by 80% (SD±0.09), 89% 74% (SD±0.09) respectively, 70% recovery. CLL lower numbers, process less ALL. This study demonstrates use depletion ALL, suggesting its potential address key bottleneck CAR-T workflow.

Язык: Английский

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