Within or Without You? A Perspective Comparing In Situ and Ex Situ Tissue Engineering Strategies for Articular Cartilage Repair DOI Creative Commons
Cathal O’Connell, Serena Duchi, Carmine Onofrillo

и другие.

Advanced Healthcare Materials, Год журнала: 2022, Номер 11(24)

Опубликована: Ноя. 22, 2022

Human articular cartilage has a poor ability to self-repair, meaning small injuries often lead osteoarthritis, painful and debilitating condition which is major contributor the global burden of disease. Existing clinical strategies generally do not regenerate hyaline type cartilage, motivating research toward tissue engineering solutions. Prospective therapies can be placed into two broad categories: i) Ex situ strategies, where constructs are engineered in lab prior implantation ii) cells and/or bioscaffold delivered defect site stimulate chondral repair directly. While commonalities exist between these approaches, core point distinction-whether chondrogenesis primarily occurs "within" or "without" (outside) body-can dictate many aspects treatment. This difference influences decisions around cell selection, biomaterials formulation surgical procedure, processes integration maturation, as well as, prospects for regulatory clearance translation. Here, ex compared: Highlighting their respective challenges, opportunities, on translational pathways long term human repair.

Язык: Английский

Chitosan hydrogel/3D-printed poly(ε‐caprolactone) hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment DOI Creative Commons
Pinxue Li, Liwei Fu,

Zhiyao Liao

и другие.

Biomaterials, Год журнала: 2021, Номер 278, С. 121131 - 121131

Опубликована: Сен. 15, 2021

Articular cartilage (AC) injury repair has always been a difficult problem for clinicians and researchers. Recently, promising therapy based on mesenchymal stem cells (MSCs) developed the regeneration of defects. As endogenous articular cells, synovial MSCs (SMSCs) possess strong chondrogenic differentiation ability specificity. In this study, regenerative system was chitosan (CS) hydrogel/3D-printed poly(ε‐caprolactone) (PCL) hybrid containing SMSCs recruiting tetrahedral framework nucleic acid (TFNA) injected into cavity. TFNA, which is DNA nanomaterial improving microenvironment, could be taken up promoted proliferation SMSCs. CS, as cationic polysaccharide, can bind to through electrostatic action recruit free TFNA after cavity injection in vivo. The 3D-printed PCL scaffold provided basic mechanical support, good microenvironment delivered regeneration, thus greatly conclusion, study confirmed that CS strategy chitosan-directed recruitment TFNA-enhanced cell chondrogenesis.

Язык: Английский

Процитировано

135
Recent advances in hyaluronic acid-based hydrogels for 3D bioprinting in tissue engineering applications DOI Creative Commons
Yanwen Ding, Xu-Wei Zhang,

Chen‐Hui Mi

и другие.

Smart Materials in Medicine, Год журнала: 2022, Номер 4, С. 59 - 68

Опубликована: Июль 31, 2022

3D bioprinting technology can rapidly process cell-loaded biomaterials to prepare personalized scaffolds for repairing defective tissues, tissue regeneration, and even printing tissues or organs. relies on bioinks with appropriate rheology cytocompatibility, hydrogels are among the most promising bioink materials bioprinting. Among many hydrogel precursor materials, hyaluronic acid (HA) stands out due its excellent physicochemical biological properties, such as biocompatibility, hydrophilicity, non-immunogenicity, complete biodegradability, has become attractive bioinks. In this review, we discuss strategies adopted application of HA-based bioinks, including printability, improving their mechanical loaded cells. Finally, summarize bioprinted in various engineering applications recent years, aim provide fresh inspiration further development

Язык: Английский

Процитировано

114
Advances in Translational 3D Printing for Cartilage, Bone, and Osteochondral Tissue Engineering DOI
Shenqiang Wang,

Sheng Zhao,

Jicheng Yu

и другие.

Small, Год журнала: 2022, Номер 18(36)

Опубликована: Июнь 17, 2022

The regeneration of 3D tissue constructs with clinically relevant sizes, structures, and hierarchical organizations for translational engineering remains challenging. printing, an additive manufacturing technique, has revolutionized the field by fabricating biomimetic precisely controlled composition, spatial distribution, architecture that can replicate both biological functional native tissues. Therefore, printing is gaining increasing attention as a viable option to advance personalized therapy various diseases regenerating desired This review outlines recently developed techniques clinical translation specifically summarizes applications these approaches cartilage, bone, osteochondral current challenges future perspectives technology are also discussed.

Язык: Английский

Процитировано

83
Emerging 3D bioprinting applications in plastic surgery DOI Creative Commons
Yang Pu,

Yikun Ju,

Yue Hu

и другие.

Biomaterials Research, Год журнала: 2023, Номер 27(1)

Опубликована: Янв. 3, 2023

Plastic surgery is a discipline that uses surgical methods or tissue transplantation to repair, reconstruct and beautify the defects deformities of human tissues organs. Three-dimensional (3D) bioprinting has gained widespread attention because it enables fine customization implants in patient's area preoperatively while avoiding some adverse reactions complications traditional approaches. In this paper, we review recent research advances application 3D plastic surgery. We first introduce printing process basic principles technology, revealing advantages disadvantages different technologies. Then, describe currently available materials, dissect rationale for special dynamic (4D bioprinting) achieved by varying combination strategy materials. Later, focus on viable clinical applications effects Finally, summarize discuss challenges prospects believe can contribute further development provide lessons related research.

Язык: Английский

Процитировано

67
Recent Advances of Self-Healing Polymer Materials via Supramolecular Forces for Biomedical Applications DOI

Jing Xie,

Peng Yu, Zhanhua Wang

и другие.

Biomacromolecules, Год журнала: 2022, Номер 23(3), С. 641 - 660

Опубликована: Фев. 24, 2022

Noncovalent interactions can maintain the three-dimensional structures of biomacromolecules (e.g., polysaccharides and proteins) control specific recognition in biological systems. Supramolecular chemistry was gradually developed as a result, this led to design application self-healing materials. Self-healing materials have attracted attention many fields, such coatings, bionic materials, elastomers, flexible electronic devices. Nevertheless, for biomedical applications not been comprehensively summarized, even though reports focused on areas. In Review, we first introduce different categories supramolecular forces used preparing then describe last 5 years, including antibiofouling, smart drug/protein delivery, wound healing, skin, cartilage lubrication protection, tissue engineering scaffolds. Finally, limitations current are indicated, key points offered new potential directions highlighted.

Язык: Английский

Процитировано

45
Articular cartilage reconstruction with TGF-β1-simulating self-assembling peptide hydrogel-based composite scaffold DOI
Weilong Ye, Zhen Yang, Fuyang Cao

и другие.

Acta Biomaterialia, Год журнала: 2022, Номер 146, С. 94 - 106

Опубликована: Май 10, 2022

Язык: Английский

Процитировано

42
Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine DOI Creative Commons

Pei‐Yao Xu,

Ranjith Kumar Kankala, Shi‐Bin Wang

и другие.

Regenerative Biomaterials, Год журнала: 2023, Номер 11

Опубликована: Дек. 1, 2023

Abstract Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering, clinical transformation of these remained a big challenge because difficulty simulating native organs/tissues’ microenvironment. As kind natural tissue-derived biomaterials, decellularized extracellular matrix (dECM)-based have gained attention due to their unique biomimetic properties, providing specific microenvironment suitable promoting cell proliferation, migration, attachment and regulating differentiation. The medical applications dECM-based addressed critical challenges, including poor mechanical strength insufficient stability. For reconstruction damaged tissues or organs, different types composite platforms been designed mimic microenvironment, by integrating with polymer or/and syntenic adding bioactive factors. In this review, we summarized research progress regenerative medicine, highlighting challenges future perspectives related application materials.

Язык: Английский

Процитировано

34
In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units DOI Creative Commons
Zhen Yang, Bin Wang, Wei Liu

и другие.

Bioactive Materials, Год журнала: 2023, Номер 27, С. 200 - 215

Опубликована: Апрель 10, 2023

The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial, directional and controllable differentiation mesenchymal stem cells (MSCs) into cartilage bone compartments. Emerging organoid technology offers new opportunities for regeneration. In this study, we developed gelatin-based microcryogels customized using hyaluronic acid (HA) hydroxyapatite (HYP), respectively (denoted as CH-Microcryogels OS-Microcryogels) through vivo self-assembly organoids. showed good cytocompatibility induced chondrogenic osteogenic MSCs, while also demonstrating the ability self-assemble organoids with no delamination biphasic cartilage-bone structure. Analysis by mRNA-seq that promoted inhibited inflammation, OS-Microcryogels facilitated suppressed immune response, regulating specific signaling pathways. Finally, engraftment pre-differentiated canine defects resulted spontaneous assembly an unit, simultaneous both articular subchondral bone. conclusion, novel approach generating self-assembling utilizing tailor-made presents a highly promising avenue advancing field tissue engineering.

Язык: Английский

Процитировано

31
Growth factor–loaded sulfated microislands in granular hydrogels promote hMSCs migration and chondrogenic differentiation DOI Creative Commons
Anna Puiggalí‐Jou, Maryam Asadikorayem, Katharina Maniura‐Weber

и другие.

Acta Biomaterialia, Год журнала: 2023, Номер 166, С. 69 - 84

Опубликована: Апрель 6, 2023

Cell-based therapies for articular cartilage lesions are expensive and time-consuming; clearly, a one-step procedure to induce endogenous repair would have significant clinical benefits. Acellular heterogeneous granular hydrogels were explored their injectability, cell-friendly cross-linking, ability promote migration, as well serve scaffold depositing extracellular matrix. The prepared by mechanical sizing of bulk methacrylated hyaluronic acid (HAMA) HAMA incorporating sulfated (SHAMA). SHAMA's negative charges allowed the retention positively charged growth factors (GFs) (e.g., TGFB3 PDGF-BB). Mixtures GF-loaded SHAMA microgels annealed enzymatic forming with GF deposits. addition loaded microislands guided cell migration enhanced chondrogenesis. Granular showed increased matrix deposition tissue maturation compared or homogeneous hydrogels. This advanced material provides an ideal 3D environment guiding differentiation into cartilage. materials which regeneration great interest defects, they more cost- time-effective current cell-based therapies. Here we develop injectable, hydrogel system promotes from surrounding tissue, facilitating repair. architecture chemistry optimized increase deposition. present study quantitative data on effect microgel size chemical modification factor maturation.

Язык: Английский

Процитировано

25
Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration DOI Creative Commons

Jiang Wu,

Liwei Fu,

Zineng Yan

и другие.

Biomaterials Research, Год журнала: 2023, Номер 27(1)

Опубликована: Фев. 5, 2023

In recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more cells from joint cavity defect area promote regeneration.In this study, we used ECM alone as a bioink low-temperature deposition manufacturing (LDM) 3D printing then successfully fabricated hierarchical porous scaffold incorporating GDF-5.Comparative vitro experiments showed that 7% scaffolds had best biocompatibility. After addition of GDF-5 protein, significantly improved bone marrow mesenchymal cell (BMSC) migration chondrogenic differentiation. Most importantly, vivo results ECM/GDF-5 enhanced repair.In conclusion, study reports construction new based concept regeneration, believe our findings will provide treatment strategy for AC repair.

Язык: Английский

Процитировано

22