Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices

Bioengineering, Год журнала: 2025, Номер 12(2), С. 136 - 136

Опубликована: Янв. 30, 2025

Whole-organ decellularization generates scaffolds containing native extracellular matrix (ECM) components with preserved tissue microarchitecture, providing a promising advancement in engineering and regenerative medicine. Decellularization retains the ECM integrity which is important for supporting cell attachment, growth, differentiation, biological function. Although there are consensus guidelines to standardize processes characterization, no specific criteria or standards regarding sterility biosafety have been established so far. This regulatory gap safety, sterilization, regulation has hampered clinical translation of decellularized scaffolds. In this review, we identify essential safe use products from both human animal sources. These include efficacy, levels chemical residue, preservation composition physical characteristics, aseptic processing assure sterility. Furthermore, explore key considerations advancing into practice, focusing on frameworks safety requirements. Addressing these challenges crucial minimizing risks adverse reactions infection transmission, thereby accelerating adoption tissue-engineered products. review aims provide foundation establishing robust guidelines, effective integration medicine applications.

Язык: Английский

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Recent advances in fabrication of dECM-based composite materials for skin tissue engineering

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Янв. 23, 2024

Chronic wound management is an intractable medical and social problem, affecting the health of millions worldwide. Decellularized extracellular matrix (dECM)-based materials possess remarkable biological properties for tissue regeneration, which have been used as commercial products skin regeneration in clinics. However, complex external environment longer chronic wound-healing process hinder application pure dECM materials. dECM-based composite are constructed to promote healing different wounds, showing noteworthy functions, such anti-microbial activity suitable degradability. Moreover, fabrication technologies designing dressings with various forms expanded This review provides a summary recent building materials, highlighting advances molded hydrogels, electrospun fibers, bio-printed scaffolds managing wounds. The associated challenges prospects clinical finally discussed.

Язык: Английский

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Differentiated mesenchymal stem cells-derived exosomes immobilized in decellularized sciatic nerve hydrogels for peripheral nerve repair

Journal of Controlled Release, Год журнала: 2024, Номер 368, С. 24 - 41

Опубликована: Фев. 21, 2024

Язык: Английский

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Constructing Tumor Organoid-like Tissue for Reliable Drug Screening Using Liver-Decellularized Extracellular Matrix Scaffolds

ACS Omega, Год журнала: 2024, Номер unknown

Опубликована: Янв. 29, 2024

The interplay between cells and their microenvironments plays a pivotal role in

Язык: Английский

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Biomimetic fabrication bioprinting strategies based on decellularized extracellular matrix for musculoskeletal tissue regeneration: Current status and future perspectives

Materials & Design, Год журнала: 2024, Номер 243, С. 113072 - 113072

Опубликована: Июнь 6, 2024

Musculoskeletal disorders, as one of the prevalent categories ailments, exert significant impacts on individuals' lives, occupations, and physical activities. Degenerative changes, injuries, infections, tumor resections causing defects in musculoskeletal tissues such cartilage, bones, skeletal muscles, menisci, ligaments, rotator cuffs can detrimentally affect patients' quality life mental well-being. Traditional autologous allogeneic transplantations have been clinically employed. However, transplantation suffers from limitation a finite number transplantable tissues, while faces challenges immune rejection. The extracellular matrix (ECM) serves natural scaffold for cells to fulfill physiological functions adhesion, proliferation, differentiation. Decellularized (dECM) emerges promising biomaterial generated through specific tissue or organ decellularization. Leveraging 3D bioprinting technology, dECM-based biomaterials enable customized printing construction. This study reviews various decellularization techniques, post-decellularization strategies, commonly used technologies. It summarizes integration with technology applied system research. These investigations showcase exciting potential system, offering prospects clinical translation orthopedics.

Язык: Английский

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Enhanced Tumor Site Accumulation and Therapeutic Efficacy of Extracellular Matrix‐Drug Conjugates Targeting Tumor Cells

Small, Год журнала: 2024, Номер unknown

Опубликована: Июнь 3, 2024

Abstract The extracellular matrix (ECM) engages in regulatory interactions with cell surface receptors through its constituent proteins and polysaccharides. Therefore, nano‐sized conjugated doxorubicin (DOX) is utilized to produce matrix‐drug conjugates (ECM‐DOX) tailored for targeted delivery cancer cells. ECM‐DOX nanoparticles exhibit rod‐like morphology, boasting a commendable drug loading capacity of 4.58%, coupled acid‐sensitive release characteristics. Notably, enhance the uptake by tumor cells possess ability penetrate endothelial infiltrate multicellular spheroids. Mechanistic insights reveal that internalization nanoparticle facilitated clathrin‐mediated endocytosis macropinocytosis, intricately involving hyaluronic acid integrins. Pharmacokinetic assessments unveil prolonged blood half‐life at 3.65 h, substantial improvement over 1.09 h observed free DOX. A sustained accumulation effect sites, levels tissues surpassing those DOX several‐fold. profound therapeutic impact evident their notable inhibition growth, extension median survival time animals, significant reduction DOX‐induced cardiotoxicity. ECM platform emerges as promising carrier avant‐garde nanomedicines realm treatment.

Язык: Английский

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Enhanced Bioprinting of 3D Corneal Stroma Patches with Reliability, Assessing Product Consistency and Quality through Optimized Electron Beam Sterilization

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

This study focuses on the optimization of sterilization methods for bioprinted three-dimensional (3D) corneal stroma patches prepared using cornea-derived decellularized extracellular matrix (Co-dECM) hydrogels and human keratocytes, with aim enhancing clinical applications in tissue engineering. An essential aspect this is to refine processes, particularly focusing electron beam (EB) sterilization, maintain structural functional integrity Co-dECM while ensuring sterility. The reveals that EB outperformed traditional like ethylene oxide (EtO) gas autoclaving, which tend degrade biochemical properties hydrogels. By optimizing EB-sterilization process, mechanical characteristics needed successful 3D bioprinting are retained, reducing batch variability patches. Consistency production vital meeting regulatory standards patient safety. Moreover, investigates immunomodulatory sterilized hydrogels, emphasizing their potential minimize inflammatory responses, crucial maintaining keratocyte phenotype. These findings significantly advance biomedical engineering by providing a method preserves material integrity, minimizes immunogenicity, supports translation patches, offering promising alternative donor transplants synthetic substitutes.

Язык: Английский

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Biomaterials for neuroengineering: Applications and challenges

Regenerative Biomaterials, Год журнала: 2025, Номер 12

Опубликована: Янв. 1, 2025

Abstract Neurological injuries and diseases are a leading cause of disability worldwide, underscoring the urgent need for effective therapies. Neural regaining enhancement therapies seen as most promising strategies restoring neural function, offering hope individuals affected by these conditions. Despite their promise, path from animal research to clinical application is fraught with challenges. Neuroengineering, particularly through use biomaterials, has emerged key field that paving way innovative solutions It seeks understand treat neurological disorders, unravel nature consciousness, explore mechanisms memory brain’s relationship behavior, tissue engineering, interfaces targeted drug delivery systems. These including both natural synthetic types, designed replicate cellular environment brain, thereby facilitating repair. This review aims provide comprehensive overview biomaterials in neuroengineering, highlighting functional across basic practice. covers recent developments biomaterial-based products, 2D 3D bioprinted scaffolds cell organoid culture, brain-on-a-chip systems, biomimetic electrodes brain–computer interfaces. also explores artificial synapses networks, discussing applications modeling microenvironments repair regeneration, modulation manipulation integration traditional Chinese medicine. serves guide role advancing neuroengineering solutions, providing insights into ongoing efforts bridge gap between innovation application.

Язык: Английский

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Evaluation of the effects of cartilage decellularized ECM in optimizing PHB-chitosan-HNT/chitosan-ECM core-shell electrospun scaffold: Physicochemical and biological properties

Biomaterials Advances, Год журнала: 2025, Номер unknown, С. 214249 - 214249

Опубликована: Фев. 1, 2025

Язык: Английский

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Stem Cell Therapies in Canine Cardiology: Comparative Efficacy, Emerging Trends, and Clinical Integration

Biomolecules, Год журнала: 2025, Номер 15(3), С. 371 - 371

Опубликована: Март 4, 2025

Cardiovascular diseases are a leading cause of morbidity and mortality in dogs, with limited options available for reversing myocardial damage. Stem cell therapies have shown significant potential cardiac repair, owing to their immunomodulatory, antifibrotic, regenerative properties. This review evaluates the therapeutic applications mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue, Wharton’s jelly focus on role canine cardiology immunoregulatory Preclinical studies highlighted efficacy enhancing function, reducing fibrosis, promoting angiogenesis. Various delivery methods, including intracoronary intramyocardial injections, assessed safety efficacy. Challenges such as low retention, differentiation efficiency, variability responses also discussed. Emerging strategies, genetic modifications combination therapies, aim enhance MSCs. Additionally, advances systems regulatory frameworks reviewed support clinical translation. comprehensive evaluation underscores revolutionize cardiovascular disease management while identifying critical areas future research integration.

Язык: Английский

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