LRET-Based Simultaneous Detection of Dual miRNAs via Multitrap Optical Tweezers Assisted Suspension Array Tagged by Two Different Luminescent Quenchable UCNPs Combining CRISPR/Cas12a Amplification DOI

He Yu,

Pengfei Xu, Yang Liu

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 602 - 612

Опубликована: Дек. 23, 2024

Nowadays, optical tweezers play a vital role not only in manipulation but also bioassay. As principal trapping objects, microbeads can combine with suspension array technology, amply focused laser beams and adequately concentrated tags contributing to highly sensitive detection. In view of the inefficiency conventional single-trap tweezers, multitrap systems are developed. Here, green- blue-emitting core-shell-shell upconversion nanoparticles (UCNPs) adopted encode determine dual miRNAs, internal shells leading luminescence process facilitate quenching through resonance energy transfer (LRET). Utilizing trans cleavage CRISPR/Cas12a, quenched signals recovered amplified, causing further enhanced detection sensitivity. Ultimately, limits (LOD) 17 22 aM obtained excellent specificities verified. Furthermore, miRNAs from MCF-7, A549, MCF-10A cells extracted detected, results consistent those by PCR. Notably, miR-155 MCF-7 A549 is detectable at single-cell level. Thus, differences measured miRNA levels between imply potential this method discriminate breast cancer epithelial despite difficulty distinguishing different due similar levels.

Язык: Английский

Lanthanide-doped upconversion nanoparticles as nanoprobes for bioimaging DOI Creative Commons
Hengde Li, Haitao Liu, Ka‐Leung Wong

и другие.

Biomaterials Science, Год журнала: 2024, Номер 12(18), С. 4650 - 4663

Опубликована: Янв. 1, 2024

Upconversion nanoparticles (UCNPs) are a class of nanomaterials composed lanthanide ions with great potential for paraclinical applications, especially in laboratory and imaging sciences. UCNPs have tunable optical properties the ability to convert long-wavelength (low energy) excitation light into short-wavelength (high emission ultraviolet (UV)-visible near-infrared (NIR) spectral regions. The core-shell structure can be customized through chemical synthesis meet needs different applications. surface also tailored by conjugating small molecules and/or targeting ligands achieve high specificity selectivity, which indispensable elements biomedical Specifically, coatings enhance water dispersion, biocompatibility, efficiency UCNPs, thereby optimizing their functionality boosting performance. In this context, multimodal provide more accurate

Язык: Английский

Процитировано

6
Quantum Dots for Chemical Metrology DOI
Jing Li, Jiao Hu, An‐An Liu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Язык: Английский

Процитировано

0
Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience DOI Creative Commons

Jing Yan,

Lin Cheng, Yitong Li

и другие.

Biosensors, Год журнала: 2025, Номер 15(5), С. 283 - 283

Опубликована: Апрель 30, 2025

Single-molecule fluorescence technology stands at the forefront of scientific research as a sophisticated tool, pushing boundaries our understanding. This review comprehensively summarizes technological advancements in single-molecule detection, highlighting latest achievements development fluorescent probes, imaging systems, and biosensors. It delves into applications these cutting-edge tools drug discovery neuroscience research, encompassing design monitoring complex delivery elucidation pharmacological mechanisms pharmacokinetics, intricacies neuronal signaling synaptic function, molecular underpinnings neurodegenerative diseases. The exceptional sensitivity demonstrated underscores vast potential modern biomedical heralding its expansion other domains.

Язык: Английский

Процитировано

0
Revealing the Existence of Long-Range Liquid–Liquid Interfacial Potential in Phase-Transfer Processes DOI

Matthew Jun Kit Ow,

Edwin K. L. Yeow

The Journal of Physical Chemistry Letters, Год журнала: 2024, Номер 15(23), С. 6241 - 6248

Опубликована: Июнь 6, 2024

By employing fluorescence wide-field microscopy and a nanoparticle-based phase transfer catalyst (PTC), consisting of fluorescent silica nanoparticle functionalized with trioctylpropylammonium bromide, we demonstrate that in the presence NaOH, single nanoparticles display subdiffusive motion along axis normal to an aqueous liquid-organic liquid interface. This is because extended interfacial potential shallow well (∼1 kBT) stretches few μm into organic phase, contrast previous molecular dynamics studies reported narrow interfaces on order ∼1 nm. Spontaneous emulsification induced by NaOH results propagation water-in-oil nanoemulsions solvent creates equilibrium hybrid-solvent composition solvates PTC. A greater mobility longer residence time PTC at enhance process catalytic efficiency.

Язык: Английский

Процитировано

2
Photon upconversion of Nd3+-Yb3+-Tb3+ doped core-shell-shell-shell nanoparticles DOI

Wenfei Xun,

Zhipeng Meng,

Chuwei Xie

и другие.

CrystEngComm, Год журнала: 2024, Номер 26(27), С. 3603 - 3608

Опубликована: Янв. 1, 2024

The NaYF 4 :Yb migration layer minimized the cross-relaxation. Excited energy absorbed by Nd 3+ could sensitize co-doped Yb and activated Tb though among , thereby achieving luminescence of .

Язык: Английский

Процитировано

1
Luminescent photon-upconversion nanoparticles with advanced functionalization for smart sensing and imaging DOI
Petr Skládal, Zdeněk Farka

Microchimica Acta, Год журнала: 2024, Номер 191(9)

Опубликована: Авг. 21, 2024

Язык: Английский

Процитировано

1
LRET-Based Simultaneous Detection of Dual miRNAs via Multitrap Optical Tweezers Assisted Suspension Array Tagged by Two Different Luminescent Quenchable UCNPs Combining CRISPR/Cas12a Amplification DOI

He Yu,

Pengfei Xu, Yang Liu

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 602 - 612

Опубликована: Дек. 23, 2024

Nowadays, optical tweezers play a vital role not only in manipulation but also bioassay. As principal trapping objects, microbeads can combine with suspension array technology, amply focused laser beams and adequately concentrated tags contributing to highly sensitive detection. In view of the inefficiency conventional single-trap tweezers, multitrap systems are developed. Here, green- blue-emitting core-shell-shell upconversion nanoparticles (UCNPs) adopted encode determine dual miRNAs, internal shells leading luminescence process facilitate quenching through resonance energy transfer (LRET). Utilizing trans cleavage CRISPR/Cas12a, quenched signals recovered amplified, causing further enhanced detection sensitivity. Ultimately, limits (LOD) 17 22 aM obtained excellent specificities verified. Furthermore, miRNAs from MCF-7, A549, MCF-10A cells extracted detected, results consistent those by PCR. Notably, miR-155 MCF-7 A549 is detectable at single-cell level. Thus, differences measured miRNA levels between imply potential this method discriminate breast cancer epithelial despite difficulty distinguishing different due similar levels.

Язык: Английский

Процитировано

0